FastQCFastQC Report
Thu 2 Feb 2017
SRR4062470_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062470_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1975809
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT166660.8435025855231959No Hit
GTCCTACAGTGGACATTTCTAAATT145680.7373182326834223No Hit
CTGTAGGACGTGGAATATGGCAAGA142690.7221851909774679No Hit
CTTTAGGACGTGAAATATGGCGAGG130090.6584138446580616No Hit
GTCCTACAGTGTGCATTTCTCATTT85210.43126638252989025No Hit
CTGAAGGACCTGGAATATGGCGAGA71970.364255856714895No Hit
ATTTAGAAATGTCCACTGTAGGACG65410.3310542668851088No Hit
CTGTAGGACCTGGAATATGGCGAGA64820.3280681482876128No Hit
GTCCTTCAGTGTGCATTTCTCATTT62990.3188061194174133No Hit
TTTCTAAATTTTCCACCTTTTTCAG50070.2534151833502125No Hit
GAATATGGCAAGAAAACTGAAAATC43500.22016298134080775No Hit
GGAATATGGCGAGAAAACTGAAAAT38260.19364219922067366No Hit
TTGTAGAACAGTGTATATCAATGAG36800.18625282099636148No Hit
GATATACACTGTTCTACAAATCCCG34980.17704140430578058No Hit
ATTCCAGGTCCTTCAGTGTGCATTT33390.16899406774642692No Hit
CCATATTCCAGGTCCTTCAGTGTGC32110.16251570875524912No Hit
GCCATATTCCACGTCCTACAGTGGA31320.15851734656538158No Hit
GAAATATGGCGAGGAAAACTGAAAA31290.15836551002652582No Hit
GTGTATATCAATGAGTTACAATGAA30220.15295000680733817No Hit
ACCTGGAATATGGCGAGAAAACTGA30190.15279817026848241No Hit
CCATATTTCACGTCCTAAAGTGTGT30030.1519883753945852No Hit
GACCTGGAATATGGCGAGAAAACTG28960.14657287217539752No Hit
CTGTAGGACATGGAATATGGCAAGA28440.14394103883523154No Hit
TTCCAGGTCCTTCAGTGTGCATTTC28140.14242267344667425No Hit
GTCCACTGTAGGACGTGGAATATGG27430.1388292086937553No Hit
ATACACACTTTAGGACGTGAAATAT27420.13877859651413674No Hit
GTATCAACGCAGAGTACTTTTTTTT27290.13812063817909526No Hit
ACAGTGGACATTTCTAAATTTTCCA26300.13311003239685618No Hit
CCACTGTAGGACGTGGAATATGGCA25380.12845371187194715No Hit
GTTCTACAGTGTGGTTTTTATCATT24910.12607493942987405No Hit
GTGTATATCAATGAGTTACAATGAG24880.1259231028910183No Hit
CCTAAAGTGTGTATTTCTCATTTTC24850.1257712663521626No Hit
CACTTTAGGACGTGAAATATGGCGA24500.1239998400655124No Hit
AGTGTGTATTTCTCATTTTCCGTGA23620.11954596825907768No Hit
GTGTATTTCTCATTTTCCGTGATTT22560.11418107721950857No Hit
ATTCCACGTCCTACAGTGGACATTT22530.11402924068065283No Hit
CTACAGTGGACATTTCTAAATTTTC22320.11296638490866273No Hit
GAAATACACACTTTAGGACGTGAAA22090.11180230477743547No Hit
GTAGGACGTGGAATATGGCAAGAAA21230.1074496573302379No Hit
GATATACACTGTTCTACAATGCCGG20790.10522272142702054No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG551.1295151E-515.5441487
TGGTACG704.438134E-714.928452510
ATACGTC400.00526586114.25566718
GCGCCGA400.005269405414.25422119
ATCGTAA602.5630114E-514.25349914
GCGTAGA400.005305854614.2394181
GCGGAGC1051.3624231E-913.57682717
CCACCTT14000.013.50654513
AATCTCG1153.783498E-1013.2176211
CGCACCG1750.013.0284689
TAACGCT905.427937E-712.6627134
TTAACGC905.434613E-712.661433
TAGGACC24500.012.4818174
AAATGTC19600.012.4071217
GAGCGGA1155.304173E-912.39497515
CACCTTT15950.012.33218114
TAGAAAT20800.012.3278834
TTCCACC16300.012.23947611
TCGAACT550.00306214812.09449219
CGAAATT550.003063893212.09357113
Produced by FastQC (version 0.11.5)