##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062470_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1975809
Sequences flagged as poor quality	0
Sequence length	25
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.31888203768684	32.0	32.0	32.0	32.0	32.0
2	31.08792752740776	32.0	32.0	32.0	32.0	32.0
3	31.04794846060525	32.0	32.0	32.0	32.0	32.0
4	31.107468889958493	32.0	32.0	32.0	32.0	32.0
5	31.022445489417247	32.0	32.0	32.0	32.0	32.0
6	34.69780783466418	36.0	36.0	36.0	32.0	36.0
7	34.59303404327038	36.0	36.0	36.0	32.0	36.0
8	34.6058095696497	36.0	36.0	36.0	32.0	36.0
9	34.68207098965538	36.0	36.0	36.0	32.0	36.0
10	34.44071921931725	36.0	36.0	36.0	32.0	36.0
11	34.65986641421311	36.0	36.0	36.0	32.0	36.0
12	34.50571284977445	36.0	36.0	36.0	32.0	36.0
13	34.56869312772641	36.0	36.0	36.0	32.0	36.0
14	34.52004368843345	36.0	36.0	36.0	32.0	36.0
15	34.467268344257974	36.0	36.0	36.0	32.0	36.0
16	34.51481899313142	36.0	36.0	36.0	32.0	36.0
17	34.4804143517921	36.0	36.0	36.0	32.0	36.0
18	34.500182963029324	36.0	36.0	36.0	32.0	36.0
19	34.41508364421865	36.0	36.0	36.0	32.0	36.0
20	34.395256828974865	36.0	36.0	36.0	32.0	36.0
21	34.36251884671039	36.0	36.0	36.0	32.0	36.0
22	34.32084022291628	36.0	36.0	36.0	32.0	36.0
23	34.33316277028802	36.0	36.0	36.0	32.0	36.0
24	34.27438684609697	36.0	36.0	36.0	32.0	36.0
25	33.898356065793806	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	7.0
4	70.0
5	191.0
6	325.0
7	65.0
8	306.0
9	369.0
10	227.0
11	63.0
12	154.0
13	113.0
14	259.0
15	355.0
16	535.0
17	677.0
18	896.0
19	1169.0
20	1807.0
21	2464.0
22	3847.0
23	5639.0
24	7953.0
25	11560.0
26	16918.0
27	22765.0
28	31332.0
29	43231.0
30	57535.0
31	79746.0
32	115783.0
33	173833.0
34	421305.0
35	974310.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.1871686175261	20.519076241695664	12.387269851312874	25.90648528946536
2	12.051079595493334	21.00828044175853	46.89199995950884	20.048640003239292
3	18.714006756024897	26.308764712372916	30.68003632097293	24.29719221062925
4	10.953357431715204	17.48771078702089	40.10122940218566	31.457702379078246
5	10.681347323397715	40.23373817031506	37.034584297833646	12.050330208453579
6	29.03357459620454	39.47443042281934	19.375346222516136	12.116648758459982
7	25.868194569583448	32.5384875240001	23.486138818954544	18.10717908746191
8	22.20755970991528	43.04544376923716	20.752830230734475	13.994166290113089
9	26.65918578260517	14.971072789170787	21.537600308016536	36.83214112020751
10	15.290131235909962	29.565855860923072	35.51811556198754	19.625897341179417
11	33.607977448779124	20.390298678009163	28.98260570378061	17.019118169431096
12	25.413762083950992	23.543375524218582	34.55506987469434	16.48779251713609
13	29.871311792736787	23.51220095548308	26.808708800619424	19.807778451160708
14	18.603222864092427	25.668136211614474	27.519712171886084	28.208928752407015
15	20.012089845897535	39.43245621991156	22.193379770637485	18.362074163553423
16	18.43587052957298	28.153955520065015	34.38664902723893	19.02352492312307
17	18.32599409144577	31.84568842764555	30.94638215069347	18.88193533021521
18	18.585156989964418	27.20547879950073	37.32548717135286	16.88387703918199
19	24.211938908256467	25.584359443328676	27.505829277797964	22.697872370616892
20	23.808314888234865	29.156988057305476	29.781739757903015	17.252957296556644
21	20.83119005036947	26.937151932958276	26.939533827720087	25.292124188952165
22	22.791284257181932	32.890696361208796	27.2094253947946	17.108593986814665
23	18.40047237230236	32.101803326879605	30.568975479214604	18.92874882160343
24	23.658315569894697	27.554007441892526	31.01805535705779	17.769621631154994
25	20.625064931445063	30.24485172825335	31.08540037045765	18.044682969843937
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	273.0
1	273.0
2	675.5
3	1078.0
4	1078.0
5	1078.0
6	2254.5
7	3431.0
8	3431.0
9	3431.0
10	4017.5
11	4604.0
12	4604.0
13	4604.0
14	6232.0
15	7860.0
16	7860.0
17	7860.0
18	14607.0
19	21354.0
20	21354.0
21	21354.0
22	40523.0
23	59692.0
24	59692.0
25	59692.0
26	99611.0
27	139530.0
28	139530.0
29	139530.0
30	193124.5
31	246719.0
32	246719.0
33	246719.0
34	258960.0
35	271201.0
36	271201.0
37	271201.0
38	268213.5
39	265226.0
40	265226.0
41	265226.0
42	261172.0
43	257118.0
44	257118.0
45	257118.0
46	254840.5
47	252563.0
48	252563.0
49	252563.0
50	216611.0
51	180659.0
52	180659.0
53	180659.0
54	141520.5
55	102382.0
56	102382.0
57	102382.0
58	84840.5
59	67299.0
60	67299.0
61	67299.0
62	54033.0
63	40767.0
64	40767.0
65	40767.0
66	32879.0
67	24991.0
68	24991.0
69	24991.0
70	19040.0
71	13089.0
72	13089.0
73	13089.0
74	10288.0
75	7487.0
76	7487.0
77	7487.0
78	6071.0
79	4655.0
80	4655.0
81	4655.0
82	3229.5
83	1804.0
84	1804.0
85	1804.0
86	1343.5
87	883.0
88	883.0
89	883.0
90	605.0
91	327.0
92	327.0
93	327.0
94	215.5
95	104.0
96	104.0
97	104.0
98	408.5
99	713.0
100	713.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009312641049818075
2	0.003492240393681778
3	0.004605708345290461
4	0.014981205167098643
5	0.03507424047567351
6	0.0467150417879461
7	0.06771909632965535
8	0.08933049702678751
9	0.09560640729949099
10	0.118432500307469
11	0.11873617338518044
12	0.13331248111533048
13	0.1192929073609848
14	0.12192474070115078
15	0.11397862850103427
16	0.11134679516086829
17	0.121418618904965
18	0.12804881443499852
19	0.13057942341592735
20	0.12071004839030494
21	0.13108554521211313
22	0.1523932728315338
23	0.14115736895620984
24	0.1343753368873206
25	0.1302757503382159
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1975809.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.89845772625949
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.22478523912936	35.27316473631556
2	10.8799114809753	9.334628454641283
3	2.5542550823204686	3.2872081101302424
4	1.0644887583839828	1.8265970400645493
5	0.5740269015651637	1.2312434385264446
6	0.39215048422755644	1.009359058198081
7	0.27720143558591603	0.8324059846298603
8	0.21105518698990824	0.7243153613595495
9	0.1677651249871414	0.6477178609981372
>10	1.3089159983687022	11.352808135842766
>50	0.1754323745510043	5.310097598121879
>100	0.1465377656038559	12.645797903798117
>500	0.012804091260867843	3.764725939888162
>1k	0.009484512045087292	7.7473312676217345
>5k	7.113384033815468E-4	2.036745061868625
>10k+	4.7422560225436454E-4	2.97585404799503
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTT	16666	0.8435025855231959	No Hit
GTCCTACAGTGGACATTTCTAAATT	14568	0.7373182326834223	No Hit
CTGTAGGACGTGGAATATGGCAAGA	14269	0.7221851909774679	No Hit
CTTTAGGACGTGAAATATGGCGAGG	13009	0.6584138446580616	No Hit
GTCCTACAGTGTGCATTTCTCATTT	8521	0.43126638252989025	No Hit
CTGAAGGACCTGGAATATGGCGAGA	7197	0.364255856714895	No Hit
ATTTAGAAATGTCCACTGTAGGACG	6541	0.3310542668851088	No Hit
CTGTAGGACCTGGAATATGGCGAGA	6482	0.3280681482876128	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	6299	0.3188061194174133	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	5007	0.2534151833502125	No Hit
GAATATGGCAAGAAAACTGAAAATC	4350	0.22016298134080775	No Hit
GGAATATGGCGAGAAAACTGAAAAT	3826	0.19364219922067366	No Hit
TTGTAGAACAGTGTATATCAATGAG	3680	0.18625282099636148	No Hit
GATATACACTGTTCTACAAATCCCG	3498	0.17704140430578058	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	3339	0.16899406774642692	No Hit
CCATATTCCAGGTCCTTCAGTGTGC	3211	0.16251570875524912	No Hit
GCCATATTCCACGTCCTACAGTGGA	3132	0.15851734656538158	No Hit
GAAATATGGCGAGGAAAACTGAAAA	3129	0.15836551002652582	No Hit
GTGTATATCAATGAGTTACAATGAA	3022	0.15295000680733817	No Hit
ACCTGGAATATGGCGAGAAAACTGA	3019	0.15279817026848241	No Hit
CCATATTTCACGTCCTAAAGTGTGT	3003	0.1519883753945852	No Hit
GACCTGGAATATGGCGAGAAAACTG	2896	0.14657287217539752	No Hit
CTGTAGGACATGGAATATGGCAAGA	2844	0.14394103883523154	No Hit
TTCCAGGTCCTTCAGTGTGCATTTC	2814	0.14242267344667425	No Hit
GTCCACTGTAGGACGTGGAATATGG	2743	0.1388292086937553	No Hit
ATACACACTTTAGGACGTGAAATAT	2742	0.13877859651413674	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2729	0.13812063817909526	No Hit
ACAGTGGACATTTCTAAATTTTCCA	2630	0.13311003239685618	No Hit
CCACTGTAGGACGTGGAATATGGCA	2538	0.12845371187194715	No Hit
GTTCTACAGTGTGGTTTTTATCATT	2491	0.12607493942987405	No Hit
GTGTATATCAATGAGTTACAATGAG	2488	0.1259231028910183	No Hit
CCTAAAGTGTGTATTTCTCATTTTC	2485	0.1257712663521626	No Hit
CACTTTAGGACGTGAAATATGGCGA	2450	0.1239998400655124	No Hit
AGTGTGTATTTCTCATTTTCCGTGA	2362	0.11954596825907768	No Hit
GTGTATTTCTCATTTTCCGTGATTT	2256	0.11418107721950857	No Hit
ATTCCACGTCCTACAGTGGACATTT	2253	0.11402924068065283	No Hit
CTACAGTGGACATTTCTAAATTTTC	2232	0.11296638490866273	No Hit
GAAATACACACTTTAGGACGTGAAA	2209	0.11180230477743547	No Hit
GTAGGACGTGGAATATGGCAAGAAA	2123	0.1074496573302379	No Hit
GATATACACTGTTCTACAATGCCGG	2079	0.10522272142702054	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	5.061217961857649E-5	0.0	0.0	0.0
5	0.0	5.061217961857649E-5	0.0	0.0	0.0
6	0.0	5.061217961857649E-5	0.0	0.0	0.0
7	0.0	5.061217961857649E-5	0.0	0.0	0.0
8	0.0	5.061217961857649E-5	0.0	0.0	0.0
9	0.0	1.0122435923715298E-4	0.0	0.0	0.0
10	0.0	1.0122435923715298E-4	0.0	0.0	0.0
11	0.0	1.0122435923715298E-4	0.0	0.0	0.0
12	0.0	1.0122435923715298E-4	0.0	0.0	0.0
13	0.0	1.0122435923715298E-4	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGCG	55	1.1295151E-5	15.544148	7
TGGTACG	70	4.438134E-7	14.9284525	10
ATACGTC	40	0.005265861	14.255667	18
GCGCCGA	40	0.0052694054	14.254221	19
ATCGTAA	60	2.5630114E-5	14.253499	14
GCGTAGA	40	0.0053058546	14.239418	1
GCGGAGC	105	1.3624231E-9	13.576827	17
CCACCTT	1400	0.0	13.506545	13
AATCTCG	115	3.783498E-10	13.21762	11
CGCACCG	175	0.0	13.028468	9
TAACGCT	90	5.427937E-7	12.662713	4
TTAACGC	90	5.434613E-7	12.66143	3
TAGGACC	2450	0.0	12.481817	4
AAATGTC	1960	0.0	12.407121	7
GAGCGGA	115	5.304173E-9	12.394975	15
CACCTTT	1595	0.0	12.332181	14
TAGAAAT	2080	0.0	12.327883	4
TTCCACC	1630	0.0	12.239476	11
TCGAACT	55	0.003062148	12.094492	19
CGAAATT	55	0.0030638932	12.093571	13
>>END_MODULE