FastQCFastQC Report
Thu 2 Feb 2017
SRR4062470_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062470_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1975809
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA79780.4037839689970032No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA65670.3323701835551918No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA60340.3053938918184906No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC57410.29056452319024767No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA40520.20508055181447196No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA32920.16661529530435382No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA25190.1274920804591942No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT23560.11924229518136623No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA20100.10173048103333876No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA20060.10152803231486444No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC28150.026.025334
TGTAGGA92450.021.4887542
CTGTAGG90200.021.3212321
CAGAGCG1551.8189894E-1221.29016314
GCGATAA1158.780262E-921.04332220
GGCGATA1057.7530785E-820.95222519
TTAGGAC59750.020.914183
AGGACCT59000.020.844445
ACTGTTC17550.020.4328968
GTAGGAC94550.020.383233
GTATCAA37000.020.2201581
GGACCTG59850.020.1072926
TATAGGA6150.020.0328772
GATATAC21200.019.9284131
TACGGCG1001.1242555E-619.79985216
TAGGACG109850.019.7673074
TAGGACA20600.019.7576334
CTAGGAC4700.019.6599253
TTTAGGA65150.019.6534582
CTTTAGG62750.019.602231
Produced by FastQC (version 0.11.5)