##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062470_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1975809
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.395440045065083	32.0	32.0	32.0	32.0	32.0
2	31.54664545004097	32.0	32.0	32.0	32.0	32.0
3	31.59867426456707	32.0	32.0	32.0	32.0	32.0
4	31.673808551332645	32.0	32.0	32.0	32.0	32.0
5	31.644358842377983	32.0	32.0	32.0	32.0	32.0
6	35.27264679936168	36.0	36.0	36.0	36.0	36.0
7	35.294868076823214	36.0	36.0	36.0	36.0	36.0
8	35.23377259644024	36.0	36.0	36.0	36.0	36.0
9	35.349827842671026	36.0	36.0	36.0	36.0	36.0
10	35.25633499999241	36.0	36.0	36.0	36.0	36.0
11	35.34641303891216	36.0	36.0	36.0	36.0	36.0
12	35.29686422118737	36.0	36.0	36.0	36.0	36.0
13	35.311110031384615	36.0	36.0	36.0	36.0	36.0
14	35.29219575373936	36.0	36.0	36.0	36.0	36.0
15	35.24772030089953	36.0	36.0	36.0	36.0	36.0
16	35.28722614382261	36.0	36.0	36.0	36.0	36.0
17	35.28321664695322	36.0	36.0	36.0	36.0	36.0
18	35.292469059509294	36.0	36.0	36.0	36.0	36.0
19	35.25292981254767	36.0	36.0	36.0	36.0	36.0
20	35.23325635220813	36.0	36.0	36.0	36.0	36.0
21	35.21994990406461	36.0	36.0	36.0	36.0	36.0
22	35.19927432256863	36.0	36.0	36.0	36.0	36.0
23	35.186505881894455	36.0	36.0	36.0	36.0	36.0
24	35.17336999679625	36.0	36.0	36.0	36.0	36.0
25	35.12792380235134	36.0	36.0	36.0	36.0	36.0
26	35.11150672964846	36.0	36.0	36.0	36.0	36.0
27	35.05805571287508	36.0	36.0	36.0	36.0	36.0
28	35.02657645551771	36.0	36.0	36.0	36.0	36.0
29	34.99886021371499	36.0	36.0	36.0	36.0	36.0
30	34.95604534648845	36.0	36.0	36.0	36.0	36.0
31	34.94979980352352	36.0	36.0	36.0	36.0	36.0
32	34.91582739019814	36.0	36.0	36.0	36.0	36.0
33	34.943298162929715	36.0	36.0	36.0	36.0	36.0
34	34.9128736633956	36.0	36.0	36.0	36.0	36.0
35	34.907697049664215	36.0	36.0	36.0	36.0	36.0
36	34.8444439720641	36.0	36.0	36.0	36.0	36.0
37	34.779709475966555	36.0	36.0	36.0	36.0	36.0
38	34.73996018845951	36.0	36.0	36.0	32.0	36.0
39	34.60383923749715	36.0	36.0	36.0	32.0	36.0
40	34.67924278105829	36.0	36.0	36.0	32.0	36.0
41	34.63799537303454	36.0	36.0	36.0	32.0	36.0
42	34.639668105570934	36.0	36.0	36.0	32.0	36.0
43	34.61730207727569	36.0	36.0	36.0	32.0	36.0
44	34.553058013198644	36.0	36.0	36.0	32.0	36.0
45	34.541395954770934	36.0	36.0	36.0	32.0	36.0
46	34.412933132706655	36.0	36.0	36.0	32.0	36.0
47	34.44441593291659	36.0	36.0	36.0	32.0	36.0
48	34.38842418472636	36.0	36.0	36.0	32.0	36.0
49	34.34259232547276	36.0	36.0	36.0	32.0	36.0
50	33.94827334018622	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	7.0
21	24.0
22	89.0
23	303.0
24	879.0
25	2113.0
26	4779.0
27	9490.0
28	17102.0
29	26702.0
30	39804.0
31	58468.0
32	85991.0
33	141192.0
34	308307.0
35	1280556.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.76048583168101	21.22456149053986	12.801069501933435	26.213883175845698
2	11.815496029546711	21.43538630746579	46.41819003613368	20.330927626853818
3	18.426326982819095	26.73223679748638	31.03394172886095	23.807494490833577
4	10.857577832675123	17.97314416525079	40.33856511434051	30.83071288773358
5	10.549006051704444	40.571270372055565	36.75141347307708	12.12831010316291
6	29.382254663685252	39.56345739797287	19.028767110807888	12.02552082753399
7	26.00657553770407	32.59967567698886	23.460073043534592	17.933675741772483
8	22.29623409955112	43.14966679471548	20.60492689323715	13.949172212496249
9	26.42168347244091	14.923152997076134	21.501622879539468	37.15354065094349
10	15.357911619999706	29.8572888371295	35.150310581640234	19.634488961230563
11	33.49488741067583	20.445852812695964	29.125942841641066	16.933316934987136
12	25.486319780909998	23.59970017344794	34.35337120136612	16.56060884427594
13	29.943481379019936	23.39735267933287	26.85173516265995	19.80743077898724
14	18.588790718131154	25.710076227003725	27.548513039468897	28.152620015396224
15	20.082103077777255	39.32374030080843	22.178408945399074	18.415747676015243
16	18.49480390057946	28.333558557532633	34.04124588965836	19.13039165222954
17	18.378294663097495	32.09854798717892	30.596024210842245	18.927133138881338
18	18.77671374105493	27.356136144738684	37.01800123392494	16.849148880281444
19	24.495029630900557	25.585873938219738	27.537125299054715	22.38197113182499
20	24.06462365542418	29.30627403762206	29.569507983818273	17.059594323135485
21	20.992059455139643	26.82167152796652	27.106365038321012	25.079903978572826
22	23.129816697869078	32.4747483182838	27.347127176766577	17.048307807080544
23	18.555184230864423	32.074254140962005	30.343418822365926	19.027142805807646
24	23.92645240506547	27.624988042872566	30.648559653286323	17.79999989877564
25	20.868312675972224	30.21309245984809	30.694313063661514	18.224281800518167
26	16.95057568823707	32.04257091652078	31.934210239957405	19.072643155284748
27	18.860122613066345	31.347665690357722	30.258947094582524	19.533264601993412
28	17.674684142040046	32.16323035273147	28.238103986772	21.92398151845649
29	20.67350639662032	28.707633177093534	27.230111817488435	23.388748608797712
30	18.143555374026537	29.42414980395372	30.362550226261746	22.06974459575799
31	22.634576520301305	27.176867804529685	29.139911803215796	21.04864387195321
32	19.305054284093252	30.8528304102269	29.660862968029804	20.181252337650047
33	18.00543473584744	31.198157311764447	32.7213308573855	18.075077095002605
34	19.75378186859155	30.73237342273469	31.89063315330581	17.623211555367956
35	18.500320628157883	31.801606329356737	31.151239821258024	18.546833221227356
36	17.5745732507545	30.311330700487748	32.42469287264103	19.68940317611672
37	18.54106343275084	30.338155155685598	27.759717665017213	23.36106374654635
38	20.02966885969241	32.787025466530416	27.880124040329807	19.303181633447362
39	22.833279937483837	27.36337368642414	29.225345162411955	20.578001213680068
40	23.967448270556517	27.58287870942991	30.677357983489294	17.77231503652428
41	23.00338747318187	25.88413151271201	32.48785687280502	18.62462414130111
42	20.60963382594168	31.215365452834764	30.81527617294992	17.359724548273643
43	17.64219112272492	31.78146268186854	31.577546210185297	18.998799985221243
44	17.28623566346747	31.1677900039933	30.346658001861513	21.19931633067771
45	17.861291248293735	31.634383687896957	29.847217013385404	20.657108050423904
46	19.343751360204394	27.343119719243692	30.49337839848649	22.819750522065426
47	22.72577966797398	27.596493385747305	29.60367120506081	20.0740557412179
48	18.04921427121751	29.36655314354778	29.242705140021126	23.34152744521358
49	21.185397981282602	27.96327985144313	29.615109557654613	21.236212609619653
50	18.32343106039096	32.79456668129359	27.88214852751455	20.9998537308009
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	18.0
1	19.0
2	20.0
3	92.5
4	165.0
5	180.0
6	195.0
7	206.0
8	217.0
9	306.0
10	395.0
11	574.0
12	753.0
13	1366.0
14	1979.0
15	2695.0
16	3411.0
17	4143.0
18	4875.0
19	5713.0
20	6551.0
21	7861.5
22	9172.0
23	11277.5
24	13383.0
25	18187.0
26	22991.0
27	35383.5
28	47776.0
29	64039.5
30	80303.0
31	100798.0
32	121293.0
33	154205.0
34	187117.0
35	220220.0
36	253323.0
37	246506.0
38	239689.0
39	211836.0
40	183983.0
41	161726.0
42	139469.0
43	120393.0
44	101317.0
45	96806.5
46	92296.0
47	88019.0
48	83742.0
49	80126.5
50	76511.0
51	68685.0
52	60859.0
53	57182.5
54	53506.0
55	48544.0
56	43582.0
57	42900.0
58	42218.0
59	37177.5
60	32137.0
61	27964.5
62	23792.0
63	19149.0
64	14506.0
65	11913.0
66	9320.0
67	8298.0
68	7276.0
69	6804.0
70	6332.0
71	5075.0
72	3818.0
73	3357.5
74	2897.0
75	2120.0
76	1343.0
77	1257.0
78	1171.0
79	1016.5
80	862.0
81	665.0
82	468.0
83	416.5
84	365.0
85	303.5
86	242.0
87	175.5
88	109.0
89	71.5
90	34.0
91	24.5
92	15.0
93	10.5
94	6.0
95	5.0
96	4.0
97	2.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01568977568175871
2	0.004555096165671885
3	5.567339758043414E-4
4	0.0
5	1.0122435923715298E-4
6	5.567339758043414E-4
7	5.061217961857649E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.5183653885572946E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1975809.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.11744204687283
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.28667843654495	46.73835350439345
2	10.698357003644182	12.007288582975171
3	2.4859255431600893	4.185113478033816
4	1.008681832349773	2.264185770824876
5	0.5049164462276515	1.416730970484661
6	0.3393899923153483	1.1427418935027105
7	0.24253157087558863	0.9527175961203513
8	0.1735438763630157	0.7791070739512959
9	0.13334974487738982	0.6734921922109981
>10	0.9178879097801292	10.082467561466824
>50	0.11835815714649871	4.663955032291091
>100	0.08211454452822278	9.108029404539568
>500	0.004722824106935915	1.7772488498257393
>1k	0.003178823918129942	2.8670943375178464
>5k	3.632941620719934E-4	1.3414737518615847
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	7978	0.4037839689970032	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	6567	0.3323701835551918	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	6034	0.3053938918184906	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	5741	0.29056452319024767	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	4052	0.20508055181447196	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	3292	0.16661529530435382	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA	2519	0.1274920804591942	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT	2356	0.11924229518136623	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	2010	0.10173048103333876	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA	2006	0.10152803231486444	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	5.061217961857649E-5	0.0
18	0.0	0.0	0.0	1.0122435923715298E-4	0.0
19	0.0	0.0	0.0	1.518365388557295E-4	0.0
20	0.0	0.0	0.0	5.061217961857649E-4	0.0
21	0.0	0.0	0.0	7.591826942786473E-4	0.0
22	0.0	0.0	0.0	0.0010122435923715298	0.0
23	0.0	0.0	0.0	0.0015183653885572947	0.0
24	0.0	0.0	0.0	0.002783669879021707	0.0
25	0.0	0.0	0.0	0.0035428525733003547	0.0
26	0.0	0.0	0.0	0.0043020352675790016	0.0
27	0.0	0.0	0.0	0.006174685913466332	0.0
28	0.0	0.0	0.0	0.010426109001426757	0.0
29	0.0	0.0	0.0	0.01832160902192469	0.0
30	0.0	0.0	0.0	0.03547913791262212	0.0
31	0.0	0.0	0.0	0.08108071174895955	0.0
32	0.0	0.0	0.0	0.14242267344667425	0.0
33	0.0	0.0	0.0	0.21246993003878412	0.0
34	0.0	0.0	0.0	0.2888943212628346	0.0
35	0.0	0.0	0.0	0.3755423727698376	0.0
36	0.0	0.0	0.0	0.49675854295632826	0.0
37	0.0	0.0	0.0	0.6965754280904682	0.0
38	0.0	0.0	0.0	0.9371857299971809	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	2815	0.0	26.02533	4
TGTAGGA	9245	0.0	21.488754	2
CTGTAGG	9020	0.0	21.321232	1
CAGAGCG	155	1.8189894E-12	21.290163	14
GCGATAA	115	8.780262E-9	21.043322	20
GGCGATA	105	7.7530785E-8	20.952225	19
TTAGGAC	5975	0.0	20.91418	3
AGGACCT	5900	0.0	20.84444	5
ACTGTTC	1755	0.0	20.432896	8
GTAGGAC	9455	0.0	20.38323	3
GTATCAA	3700	0.0	20.220158	1
GGACCTG	5985	0.0	20.107292	6
TATAGGA	615	0.0	20.032877	2
GATATAC	2120	0.0	19.928413	1
TACGGCG	100	1.1242555E-6	19.799852	16
TAGGACG	10985	0.0	19.767307	4
TAGGACA	2060	0.0	19.757633	4
CTAGGAC	470	0.0	19.659925	3
TTTAGGA	6515	0.0	19.653458	2
CTTTAGG	6275	0.0	19.60223	1
>>END_MODULE