FastQCFastQC Report
Thu 2 Feb 2017
SRR4062469_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062469_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449463
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG127452.835606045436443No Hit
TCCATGTACTCTGCGTTGATACCAC99322.2097480771498432No Hit
GAGTACATGGAAGCAGTGGTATCAA52231.1620533837045541No Hit
CATGTACTCTGCGTTGATACCACTG43510.9680440881674354No Hit
GTATCAACGCAGAGTACTTTTTTTT33870.7535659220002536No Hit
GCTTCCATGTACTCTGCGTTGATAC32210.7166329597764444No Hit
CCCATGTACTCTGCGTTGATACCAC30200.6719129272042416No Hit
GTACATGGGAAGCAGTGGTATCAAC30110.6699105376860832No Hit
GTACTTTTTTTTTTTTTTTTTTTTT28970.6445469371227442No Hit
GCGTTGATACCACTGCTTCCATGTA27780.6180708979382062No Hit
CATGGAAGCAGTGGTATCAACGCAG27470.611173778486772No Hit
TATCAACGCAGAGTACTTTTTTTTT24060.5353054645209951No Hit
GTATCAACGCAGAGTACATGGAAGC21330.4745663158035256No Hit
GAGTACTTTTTTTTTTTTTTTTTTT20740.4614395400733764No Hit
ACGCAGAGTACATGGAAGCAGTGGT18490.41137980211941805No Hit
ACTCTGCGTTGATACCACTGCTTCC18260.40626258446190233No Hit
CAGTGGTATCAACGCAGAGTACATG17660.3929133210075134No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15970.35531289561098467No Hit
TATCAACGCAGAGTACATGGAAGCA14670.3263894914598087No Hit
GTACATGGGGTGGTATCAACGCAAA13000.2892340415117596No Hit
GTATCAACGCAGAGTACATGGGAAG12850.2858967256481624No Hit
GGTATCAACGCAGAGTACTTTTTTT11720.2607556128090633No Hit
GGTATCAACGCAGAGTACATGGAAG10120.22515757693069285No Hit
GAGTACATGGGAAGCAGTGGTATCA9980.22204274879133543No Hit
ATACCACTGCTTCCATGTACTCTGC9690.2155906047883808No Hit
GCGTTGATACCACTGCTTCCCATGT9330.20758104671574745No Hit
AAGCAGTGGTATCAACGCAGAGTAC8960.1993490009188743No Hit
GTACTCTGCGTTGATACCACTGCTT8060.17932510573729094No Hit
GTACATGGGTGGTATCAACGCAAAA7880.17532032670097428No Hit
GTGGTATCAACGCAGAGTACATGGA7730.17198301083737705No Hit
TATCAACGCAGAGTACATGGGAAGC7330.16308350186778445No Hit
CCACTGCTTCCATGTACTCTGCGTT7280.16197106324658536No Hit
AAAAAGTACTCTGCGTTGATACCAC7090.15774379648602888No Hit
GAAGCAGTGGTATCAACGCAGAGTA6820.15173662793155387No Hit
GCTTCCCATGTACTCTGCGTTGATA6740.14995672613763536No Hit
GGTATCAACGCAGAGTACATGGGAA6740.14995672613763536No Hit
GCAGAGTACTTTTTTTTTTTTTTTT6630.1475093611709974No Hit
ACATGGAAGCAGTGGTATCAACGCA6590.14661941027403813No Hit
GTACATGGGAGTGGTATCAACGCAA6220.138387364477165No Hit
ACGCAGAGTACATGGGAAGCAGTGG6140.13660746268324644No Hit
CTGCTTCCATGTACTCTGCGTTGAT6120.13616248723476682No Hit
GTCCTACAGTGGACATTTCTAAATT5990.13327014681964924No Hit
TGGAAGCAGTGGTATCAACGCAGAG5960.1326026836469298No Hit
GTCCTAAAGTGTGTATTTCTCATTT5780.1285979046106131No Hit
GTTGATACCACTGCTTCCATGTACT5710.12704049054093441No Hit
GCAGAGTACATGGAAGCAGTGGTAT5180.11524864115622421No Hit
CTGTAGGACGTGGAATATGGCAAGA5030.11191132529262697No Hit
CCATAGGGTCTTCTCGTCTTATTAT5010.11146634984414736No Hit
GTATTAGAGGCACTGCCTGCCCAGT4920.10946396032598901No Hit
GATACCACTGCTTCCATGTACTCTG4910.10924147260174918No Hit
CTTCCATGTACTCTGCGTTGATACC4900.1090189848775094No Hit
CCCCATGTACTCTGCGTTGATACCA4730.10523669356543251No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG307.6946453E-419.00601618
CCAACGA307.6946453E-419.00601619
TGTCGAG250.00601453719.00601618
GTGAGCA400.005264199314.2529239
TAGGACC954.8748916E-913.9981954
CGAGGGT551.953716E-413.81947817
CAGGAGT551.9574119E-413.81644
ATCTTTG500.001495595413.3012477
GACATGG500.001495595413.3012477
TAATAGC500.001498108413.29828555
CAGTACT500.001498108413.29828554
AACGAGG604.0787365E-412.66785415
AATACTT751.4750023E-512.6650335
GTCCTAG751.4783844E-512.6622131
CCTTTAG604.0940955E-412.6622131
GATATAC751.4783844E-512.6622131
CCAATTT701.0866203E-412.21543114
AGTATTA550.003060456612.09204315
TAGGCTG550.003065567512.089355
TCCTACA2750.012.0839682
Produced by FastQC (version 0.11.5)