##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062469_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	449463
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.787279486854313	32.0	32.0	32.0	32.0	32.0
2	30.689700820757214	32.0	32.0	32.0	32.0	32.0
3	30.6985002102509	32.0	32.0	32.0	32.0	32.0
4	30.66500690824384	32.0	32.0	32.0	32.0	32.0
5	30.637591970863006	32.0	32.0	32.0	32.0	32.0
6	34.14122185808398	36.0	36.0	36.0	32.0	36.0
7	33.894614239659326	36.0	36.0	36.0	32.0	36.0
8	33.91854279440132	36.0	36.0	36.0	32.0	36.0
9	33.96660681746884	36.0	36.0	36.0	32.0	36.0
10	33.82088180784625	36.0	36.0	36.0	32.0	36.0
11	34.146866371647945	36.0	36.0	36.0	32.0	36.0
12	33.96451320798375	36.0	36.0	36.0	32.0	36.0
13	33.98923381902404	36.0	36.0	36.0	32.0	36.0
14	33.93245272692079	36.0	36.0	36.0	32.0	36.0
15	33.94192847909616	36.0	36.0	36.0	32.0	36.0
16	33.86486540605122	36.0	36.0	36.0	32.0	36.0
17	33.80734342982626	36.0	36.0	36.0	32.0	36.0
18	33.82409898033876	36.0	36.0	36.0	32.0	36.0
19	33.826059097189315	36.0	36.0	36.0	32.0	36.0
20	33.76059208433175	36.0	36.0	36.0	32.0	36.0
21	33.619904196785946	36.0	36.0	36.0	27.0	36.0
22	33.58301573210698	36.0	36.0	36.0	27.0	36.0
23	33.4609634163435	36.0	36.0	36.0	27.0	36.0
24	33.47896044835726	36.0	36.0	36.0	27.0	36.0
25	32.881847894042444	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	6.0
4	14.0
5	56.0
6	78.0
7	28.0
8	60.0
9	75.0
10	52.0
11	12.0
12	37.0
13	37.0
14	177.0
15	204.0
16	303.0
17	408.0
18	523.0
19	779.0
20	1187.0
21	1562.0
22	2228.0
23	3061.0
24	3744.0
25	5033.0
26	6387.0
27	7750.0
28	10258.0
29	13475.0
30	17016.0
31	23094.0
32	32483.0
33	45138.0
34	94086.0
35	180112.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.160982252523276	17.965982519536468	12.809735300923867	22.063299927016395
2	11.762507092080233	23.07690596179733	41.02481949960507	24.135767446517374
3	19.225078375485882	30.627515636159746	28.35045467794295	21.796951310411426
4	10.743126717625199	18.00215850551309	37.25224473447044	34.002470042391266
5	10.378351669630597	41.670765717541634	34.86048017877055	13.090402434057214
6	29.096538813415574	35.50760285727642	21.678215827210014	13.717642502097993
7	26.13272535390014	33.89804469895667	23.092679755442646	16.876550191700545
8	28.562839181488403	32.83399286062633	22.551379662806	16.051788295079266
9	28.16320803880433	16.44373772331868	19.780990686425167	35.612063551451826
10	14.790533345808216	30.308842464502273	34.494388530235994	20.40623565945351
11	33.003196667446346	24.12158474510197	22.04987692273421	20.825341664717477
12	21.77893334402752	27.537975501401384	31.525570245208783	19.15752090936231
13	30.501836748787568	23.153504971139988	25.94438293483273	20.400275345239713
14	23.531822941032324	21.430417251442446	27.88077257234512	27.156987235180114
15	24.892240179098494	27.718387668044066	29.36970250492855	18.019669647928897
16	23.16091710789806	27.8039261983414	29.299026803554206	19.736129890206332
17	21.092004339680226	27.28651914427209	31.00465379465827	20.61682272138941
18	22.397510075771585	25.194441783316883	33.857266029332806	18.550782111578727
19	20.7364647641624	32.165971923617626	28.677032485457183	18.420530826762793
20	21.467715777655005	25.08242590579387	34.96930191940974	18.48055639714138
21	22.33717857890774	28.641287154741057	25.964764539974066	23.056769726377137
22	20.035297420881633	30.804353348575404	27.675495478632133	21.48485375191083
23	19.48200105168403	29.776249766044867	27.497081131184213	23.24466805108689
24	18.84346314945192	32.030122092505124	28.335264236699047	20.79115052134391
25	23.005901919850636	27.616707178775368	27.029188770088027	22.34820213128597
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	199.0
1	199.0
2	480.5
3	762.0
4	762.0
5	762.0
6	2414.0
7	4066.0
8	4066.0
9	4066.0
10	3835.0
11	3604.0
12	3604.0
13	3604.0
14	3135.5
15	2667.0
16	2667.0
17	2667.0
18	4241.5
19	5816.0
20	5816.0
21	5816.0
22	9030.0
23	12244.0
24	12244.0
25	12244.0
26	17527.0
27	22810.0
28	22810.0
29	22810.0
30	27572.5
31	32335.0
32	32335.0
33	32335.0
34	34684.5
35	37034.0
36	37034.0
37	37034.0
38	38746.0
39	40458.0
40	40458.0
41	40458.0
42	47445.0
43	54432.0
44	54432.0
45	54432.0
46	80689.5
47	106947.0
48	106947.0
49	106947.0
50	83810.5
51	60674.0
52	60674.0
53	60674.0
54	45151.5
55	29629.0
56	29629.0
57	29629.0
58	23314.5
59	17000.0
60	17000.0
61	17000.0
62	13114.0
63	9228.0
64	9228.0
65	9228.0
66	7051.5
67	4875.0
68	4875.0
69	4875.0
70	3668.5
71	2462.0
72	2462.0
73	2462.0
74	1855.5
75	1249.0
76	1249.0
77	1249.0
78	851.5
79	454.0
80	454.0
81	454.0
82	294.5
83	135.0
84	135.0
85	135.0
86	106.0
87	77.0
88	77.0
89	77.0
90	51.5
91	26.0
92	26.0
93	26.0
94	26.5
95	27.0
96	27.0
97	27.0
98	140.0
99	253.0
100	253.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010456923039271308
2	0.004004779036316671
3	0.005339705381755561
4	0.017354042490705573
5	0.03826788856924819
6	0.04983725022971858
7	0.0723085103779399
8	0.0898850405928853
9	0.09811708638975845
10	0.12481561329853626
11	0.1241481501258168
12	0.13816487675292516
13	0.12726297826517421
14	0.12748546598941404
15	0.12192327288341866
16	0.11791849384710199
17	0.12948785550757236
18	0.13660746268324644
19	0.1370524381317261
20	0.12793044143789364
21	0.1328251713711696
22	0.15685384558906962
23	0.14661941027403813
24	0.1386098522014048
25	0.13927731537412424
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	449463.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.20196595281266
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.59869268798293	38.516187140521936
2	11.808958183543721	11.148120842356384
3	3.1076327535499324	4.400591262807281
4	1.1675160944199459	2.204362197526844
5	0.5607035953358586	1.3233156008331421
6	0.30744688180926416	0.8707258348475584
7	0.1961077762392198	0.6479670803987818
8	0.14860625343458203	0.5611605851995352
9	0.09865220918106248	0.41909203970408365
>10	0.7717095347882479	7.481185044013243
>50	0.11580532783886041	3.903564082653203
>100	0.09548031111611124	8.310245278153419
>500	0.01181687018763753	3.881922237887382
>1k	0.009453496150110024	10.1067415054923
>5k	9.453496150110025E-4	3.3812593548585137
>10k+	4.7267480750550124E-4	2.843559912746404
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	12745	2.835606045436443	No Hit
TCCATGTACTCTGCGTTGATACCAC	9932	2.2097480771498432	No Hit
GAGTACATGGAAGCAGTGGTATCAA	5223	1.1620533837045541	No Hit
CATGTACTCTGCGTTGATACCACTG	4351	0.9680440881674354	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3387	0.7535659220002536	No Hit
GCTTCCATGTACTCTGCGTTGATAC	3221	0.7166329597764444	No Hit
CCCATGTACTCTGCGTTGATACCAC	3020	0.6719129272042416	No Hit
GTACATGGGAAGCAGTGGTATCAAC	3011	0.6699105376860832	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2897	0.6445469371227442	No Hit
GCGTTGATACCACTGCTTCCATGTA	2778	0.6180708979382062	No Hit
CATGGAAGCAGTGGTATCAACGCAG	2747	0.611173778486772	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2406	0.5353054645209951	No Hit
GTATCAACGCAGAGTACATGGAAGC	2133	0.4745663158035256	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2074	0.4614395400733764	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	1849	0.41137980211941805	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1826	0.40626258446190233	No Hit
CAGTGGTATCAACGCAGAGTACATG	1766	0.3929133210075134	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1597	0.35531289561098467	No Hit
TATCAACGCAGAGTACATGGAAGCA	1467	0.3263894914598087	No Hit
GTACATGGGGTGGTATCAACGCAAA	1300	0.2892340415117596	No Hit
GTATCAACGCAGAGTACATGGGAAG	1285	0.2858967256481624	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1172	0.2607556128090633	No Hit
GGTATCAACGCAGAGTACATGGAAG	1012	0.22515757693069285	No Hit
GAGTACATGGGAAGCAGTGGTATCA	998	0.22204274879133543	No Hit
ATACCACTGCTTCCATGTACTCTGC	969	0.2155906047883808	No Hit
GCGTTGATACCACTGCTTCCCATGT	933	0.20758104671574745	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	896	0.1993490009188743	No Hit
GTACTCTGCGTTGATACCACTGCTT	806	0.17932510573729094	No Hit
GTACATGGGTGGTATCAACGCAAAA	788	0.17532032670097428	No Hit
GTGGTATCAACGCAGAGTACATGGA	773	0.17198301083737705	No Hit
TATCAACGCAGAGTACATGGGAAGC	733	0.16308350186778445	No Hit
CCACTGCTTCCATGTACTCTGCGTT	728	0.16197106324658536	No Hit
AAAAAGTACTCTGCGTTGATACCAC	709	0.15774379648602888	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	682	0.15173662793155387	No Hit
GCTTCCCATGTACTCTGCGTTGATA	674	0.14995672613763536	No Hit
GGTATCAACGCAGAGTACATGGGAA	674	0.14995672613763536	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	663	0.1475093611709974	No Hit
ACATGGAAGCAGTGGTATCAACGCA	659	0.14661941027403813	No Hit
GTACATGGGAGTGGTATCAACGCAA	622	0.138387364477165	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	614	0.13660746268324644	No Hit
CTGCTTCCATGTACTCTGCGTTGAT	612	0.13616248723476682	No Hit
GTCCTACAGTGGACATTTCTAAATT	599	0.13327014681964924	No Hit
TGGAAGCAGTGGTATCAACGCAGAG	596	0.1326026836469298	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	578	0.1285979046106131	No Hit
GTTGATACCACTGCTTCCATGTACT	571	0.12704049054093441	No Hit
GCAGAGTACATGGAAGCAGTGGTAT	518	0.11524864115622421	No Hit
CTGTAGGACGTGGAATATGGCAAGA	503	0.11191132529262697	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	501	0.11146634984414736	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	492	0.10946396032598901	No Hit
GATACCACTGCTTCCATGTACTCTG	491	0.10924147260174918	No Hit
CTTCCATGTACTCTGCGTTGATACC	490	0.1090189848775094	No Hit
CCCCATGTACTCTGCGTTGATACCA	473	0.10523669356543251	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	2.2248772423981506E-4	0.0
8	0.0	0.0	0.0	2.2248772423981506E-4	0.0
9	0.0	0.0	0.0	2.2248772423981506E-4	0.0
10	0.0	0.0	0.0	2.2248772423981506E-4	0.0
11	0.0	0.0	0.0	2.2248772423981506E-4	0.0
12	0.0	0.0	0.0	4.4497544847963013E-4	0.0
13	0.0	0.0	0.0	4.4497544847963013E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCAACG	30	7.6946453E-4	19.006016	18
CCAACGA	30	7.6946453E-4	19.006016	19
TGTCGAG	25	0.006014537	19.006016	18
GTGAGCA	40	0.0052641993	14.252923	9
TAGGACC	95	4.8748916E-9	13.998195	4
CGAGGGT	55	1.953716E-4	13.819478	17
CAGGAGT	55	1.9574119E-4	13.8164	4
ATCTTTG	50	0.0014955954	13.301247	7
GACATGG	50	0.0014955954	13.301247	7
TAATAGC	50	0.0014981084	13.2982855	5
CAGTACT	50	0.0014981084	13.2982855	4
AACGAGG	60	4.0787365E-4	12.667854	15
AATACTT	75	1.4750023E-5	12.665033	5
GTCCTAG	75	1.4783844E-5	12.662213	1
CCTTTAG	60	4.0940955E-4	12.662213	1
GATATAC	75	1.4783844E-5	12.662213	1
CCAATTT	70	1.0866203E-4	12.215431	14
AGTATTA	55	0.0030604566	12.092043	15
TAGGCTG	55	0.0030655675	12.08935	5
TCCTACA	275	0.0	12.083968	2
>>END_MODULE