FastQCFastQC Report
Thu 2 Feb 2017
SRR4062469_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062469_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449463
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA56081.2477111575368829No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC48781.0852951188418178No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA21330.4745663158035256No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC19560.4351859886130783No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG12940.2878991151663207No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC12400.27588477805737066No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA12320.2741048762634522No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG11970.26631780591505866No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11680.259865661912104No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11500.2558608828757873No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA11500.2558608828757873No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA9390.20891597306118637No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATA9380.20869348533694654No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8180.18199495842816873No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC7620.16953564587073908No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG6750.15017921386187516No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA6270.13949980309836404No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG6110.13593999951052702No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG5710.12704049054093441No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5620.12503810102277607No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTCTCTTATA5170.11502615343198438No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT5160.11480366570774457No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5100.11346873936230568No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA5080.11302376391382607No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA4840.1076840585320705No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT4710.1047917181169529No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGTCTCTTATA4690.10434674266847327No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG4620.10278932859879455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATA601.9826984E-1036.6663944
TGCGATT250.002351313635.1997344
CGGACGG300.005741436529.3331128
TTCGTAC703.5804987E-828.28549834
CGCGGTA858.20728E-925.88215643
AATTCGT801.3061072E-724.74981332
TCGTACA801.3061072E-724.74981335
GTCCATA450.001395100824.44971
ACTAGAT450.00139691124.444263
CTCGTTA551.5915291E-423.99981713
CCGTTTC651.810212E-523.69212723
TGGTATA3750.023.46648844
CGTACAT852.3460052E-723.2939436
AAATTCG904.0703162E-721.99983431
TAGGAGC904.0703162E-721.99983444
TCCGGTC1205.9480953E-1021.99983423
AATCCCG703.209751E-521.99983219
ATCTAGG1004.608046E-821.99983241
TATGCGT2000.021.99983239
CCCGTTT703.209751E-521.99983222
Produced by FastQC (version 0.11.5)