##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062469_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	449463
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.04385455532491	32.0	32.0	32.0	32.0	32.0
2	31.46082992370896	32.0	32.0	32.0	32.0	32.0
3	31.543646529302745	32.0	32.0	32.0	32.0	32.0
4	31.647917626144977	32.0	32.0	32.0	32.0	32.0
5	31.619054293679348	32.0	32.0	32.0	32.0	32.0
6	35.19589154168419	36.0	36.0	36.0	36.0	36.0
7	35.20019222939374	36.0	36.0	36.0	36.0	36.0
8	35.19026927689265	36.0	36.0	36.0	36.0	36.0
9	35.23199462469658	36.0	36.0	36.0	36.0	36.0
10	35.175329226209946	36.0	36.0	36.0	36.0	36.0
11	35.244436138236075	36.0	36.0	36.0	36.0	36.0
12	35.170610261578815	36.0	36.0	36.0	36.0	36.0
13	35.18161227954248	36.0	36.0	36.0	36.0	36.0
14	35.14649704202571	36.0	36.0	36.0	36.0	36.0
15	35.150650887837266	36.0	36.0	36.0	36.0	36.0
16	35.118637129196394	36.0	36.0	36.0	36.0	36.0
17	35.108226928579214	36.0	36.0	36.0	36.0	36.0
18	35.09563189851	36.0	36.0	36.0	36.0	36.0
19	35.053650689823186	36.0	36.0	36.0	36.0	36.0
20	35.00568456135433	36.0	36.0	36.0	36.0	36.0
21	34.90434140296309	36.0	36.0	36.0	36.0	36.0
22	34.876074337598425	36.0	36.0	36.0	32.0	36.0
23	34.76249657925124	36.0	36.0	36.0	32.0	36.0
24	34.755301326249324	36.0	36.0	36.0	32.0	36.0
25	34.710165241632794	36.0	36.0	36.0	32.0	36.0
26	34.63245695418755	36.0	36.0	36.0	32.0	36.0
27	34.565261211712645	36.0	36.0	36.0	32.0	36.0
28	34.48388187681745	36.0	36.0	36.0	32.0	36.0
29	34.42087335331273	36.0	36.0	36.0	32.0	36.0
30	34.378531714512654	36.0	36.0	36.0	32.0	36.0
31	34.31199008594701	36.0	36.0	36.0	32.0	36.0
32	34.25262146161086	36.0	36.0	36.0	32.0	36.0
33	34.15018366361636	36.0	36.0	36.0	32.0	36.0
34	34.069620858669126	36.0	36.0	36.0	32.0	36.0
35	33.91844712467989	36.0	36.0	36.0	32.0	36.0
36	33.83473389355742	36.0	36.0	36.0	32.0	36.0
37	33.689952231885606	36.0	36.0	36.0	27.0	36.0
38	33.544474183636915	36.0	36.0	36.0	21.0	36.0
39	33.20787028075726	36.0	36.0	36.0	14.0	36.0
40	32.9867286072491	36.0	36.0	36.0	14.0	36.0
41	33.056945733019184	36.0	36.0	36.0	14.0	36.0
42	32.66895606534909	36.0	36.0	36.0	14.0	36.0
43	32.73518176134632	36.0	36.0	36.0	14.0	36.0
44	32.755114436560966	36.0	36.0	36.0	14.0	36.0
45	32.31541417202306	36.0	36.0	36.0	14.0	36.0
46	32.56314535345512	36.0	36.0	36.0	14.0	36.0
47	32.29992457666148	36.0	36.0	36.0	14.0	36.0
48	32.3787230539555	36.0	36.0	36.0	14.0	36.0
49	32.442107581714176	36.0	36.0	36.0	14.0	36.0
50	31.72713883011505	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	7.0
21	19.0
22	84.0
23	253.0
24	671.0
25	1627.0
26	3590.0
27	6725.0
28	11263.0
29	15222.0
30	19495.0
31	24771.0
32	35348.0
33	36912.0
34	62378.0
35	231098.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.3049480626986	18.530534860743945	13.448512198812606	22.716004877744844
2	11.475220163849073	23.39677552854899	40.17617557927901	24.951828728322926
3	18.593521602445584	30.21828270625168	29.053114494407772	22.135081196894962
4	10.676740910820245	18.470263403216727	37.17747623274886	33.67551945321417
5	10.206401861777277	41.316637854506375	35.257407172559255	13.219553111157092
6	29.41618831486673	35.79183909580385	21.000311484892983	13.791661104436434
7	25.716466093983264	33.77919873270992	23.237062895054766	17.267272278252047
8	28.409679995906227	33.1079532686784	22.588066203447223	15.894300531968147
9	27.884163991251782	15.95303729116746	19.79250794837395	36.37029076920681
10	14.69130940700347	30.108818745925692	35.00466111782282	20.195210729248014
11	33.11907765489039	23.536086396433078	22.095923357428752	21.24891259124778
12	21.337462705495224	27.55488215937686	31.844000507272014	19.263654627855907
13	30.959611803418746	22.744030098139334	26.14253008590251	20.153828012539407
14	23.350086658968593	21.190398319772708	27.990735611162656	27.46877941009605
15	25.009400106349133	27.60850170091865	29.219090336690673	18.163007856041542
16	23.192787837931043	27.282779672631563	29.519448764414424	20.00498372502297
17	20.662657437875865	27.210471162253626	31.51249379815469	20.614377601715823
18	22.642798183610218	24.371527800953583	34.238190907816666	18.747483107619537
19	20.87179589866129	31.773026923239513	28.996380124726617	18.35879705337258
20	21.40398653504293	24.761993756994457	35.280545895880195	18.55347381208242
21	22.41074348722809	28.313342811310388	26.237532344152914	23.03838135730861
22	20.062830533325325	30.154428729394855	28.011426969516958	21.771313767762866
23	19.58759675434908	28.7084810095603	27.889058721185055	23.814863514905564
24	19.03070998057682	31.399247546516623	28.626828014764282	20.943214458142272
25	23.22972080015485	26.487163570749985	27.307920785470664	22.975194843624504
26	18.303620097761105	26.810660721794676	30.513078940869438	24.372640239574782
27	22.933144663743178	29.707895866845547	29.195506637921255	18.163452831490023
28	20.915625980336536	25.710013949980308	29.4969775042662	23.87738256541695
29	19.467008407811097	29.2566907620872	32.023770588457786	19.252530241643917
30	23.76146646108801	26.32230016708828	30.67794234453114	19.23829102729257
31	19.06742045507639	27.49859276514418	29.902350137831146	23.531636641948282
32	18.49028729839831	29.496087553369243	30.631442410165022	21.382182738067428
33	19.039164514097934	28.684674823066636	30.18290715809755	22.093253504737874
34	17.800575353254885	30.314397403123284	32.655413237574614	19.229614006047218
35	22.27035373323277	28.6548614680185	29.573958256853178	19.50082654189555
36	20.714052102175263	29.353472922131523	30.250765913990698	19.68170906170252
37	22.21028204768802	29.058231712065286	29.46582922287263	19.265657017374068
38	20.45196156302076	29.99090025207859	27.1661961051299	22.39094207977075
39	19.53775950411936	29.204628634615087	30.49683733699993	20.760774524265624
40	22.21094951086074	28.783459372629117	27.834326741022064	21.171264375488082
41	19.060745823349194	28.342266215461564	30.146864146770703	22.45012381441854
42	22.721336350266874	29.51544398537811	29.068688635104557	18.69453102925046
43	21.367276060543357	27.245401734959273	27.7669129605774	23.62040924391997
44	20.72806882880237	27.69460445019946	32.31077975272714	19.266546968271026
45	22.453683618006377	27.828319572467592	29.341236097298328	20.376760712227703
46	20.64352349359124	27.66078631611501	31.009004078199986	20.686686112093767
47	22.660152226100923	26.505185076413408	30.995877302469836	19.83878539501583
48	19.25030536440152	33.513770877691826	26.911892636323792	20.324031121582866
49	19.170209783675183	27.412712503587617	33.24856551039797	20.168512202339237
50	18.420467136265135	33.05507473379284	25.88049712767709	22.64396100226493
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	19.0
1	19.0
2	19.0
3	98.0
4	177.0
5	210.5
6	244.0
7	244.0
8	244.0
9	340.5
10	437.0
11	642.0
12	847.0
13	1469.0
14	2091.0
15	2864.0
16	3637.0
17	4285.0
18	4933.0
19	5301.0
20	5669.0
21	6215.5
22	6762.0
23	6883.5
24	7005.0
25	7076.5
26	7148.0
27	8408.0
28	9668.0
29	10844.0
30	12020.0
31	13008.0
32	13996.0
33	16329.0
34	18662.0
35	20932.0
36	23202.0
37	25069.0
38	26936.0
39	26685.5
40	26435.0
41	27539.0
42	28643.0
43	30412.5
44	32182.0
45	38733.5
46	45285.0
47	49006.0
48	52727.0
49	45989.5
50	39252.0
51	31655.0
52	24058.0
53	19712.5
54	15367.0
55	13629.5
56	11892.0
57	10666.0
58	9440.0
59	7990.0
60	6540.0
61	5517.5
62	4495.0
63	3787.0
64	3079.0
65	2673.0
66	2267.0
67	1865.0
68	1463.0
69	1252.5
70	1042.0
71	839.5
72	637.0
73	538.5
74	440.0
75	340.5
76	241.0
77	182.0
78	123.0
79	104.0
80	85.0
81	55.5
82	26.0
83	18.5
84	11.0
85	9.5
86	8.0
87	5.5
88	3.0
89	2.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	1.0
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01668657931798613
2	0.0055621931059953765
3	0.0
4	0.0
5	0.0
6	6.674631727194452E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	2.2248772423981506E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	449463.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.60835928893646
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.0623681689297	48.68149117034552
2	11.038798456975679	12.93931732169176
3	2.7514186773764298	4.837684031939045
4	1.06151356425705	2.488542734562269
5	0.48972139770695644	1.4350883814144724
6	0.2959113573744346	1.0405727490406644
7	0.19127410661272753	0.7847183094120351
8	0.1402642489532382	0.6576525998435371
9	0.09903241043168357	0.5223714382646577
>10	0.6969047035941182	8.05149763280884
>50	0.09748272453309106	4.031799187192937
>100	0.06613533859445572	7.458212354963995
>500	0.004969707524438869	1.916532939328347
>1k	0.0038228519418760532	3.898040691890879
>5k	3.8228519418760524E-4	1.2564784573010719
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	5608	1.2477111575368829	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	4878	1.0852951188418178	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	2133	0.4745663158035256	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	1956	0.4351859886130783	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	1294	0.2878991151663207	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	1240	0.27588477805737066	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	1232	0.2741048762634522	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	1197	0.26631780591505866	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	1168	0.259865661912104	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	1150	0.2558608828757873	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1150	0.2558608828757873	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	939	0.20891597306118637	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATA	938	0.20869348533694654	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	818	0.18199495842816873	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC	762	0.16953564587073908	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	675	0.15017921386187516	No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA	627	0.13949980309836404	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	611	0.13593999951052702	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	571	0.12704049054093441	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	562	0.12503810102277607	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTCTCTTATA	517	0.11502615343198438	No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT	516	0.11480366570774457	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	510	0.11346873936230568	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	508	0.11302376391382607	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	484	0.1076840585320705	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	471	0.1047917181169529	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGTCTCTTATA	469	0.10434674266847327	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	462	0.10278932859879455	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	2.2248772423981506E-4	0.0
13	0.0	0.0	0.0	2.2248772423981506E-4	0.0
14	0.0	0.0	0.0	2.2248772423981506E-4	0.0
15	0.0	0.0	0.0	6.674631727194452E-4	0.0
16	0.0	0.0	0.0	8.899508969592603E-4	0.0
17	0.0	0.0	0.0	8.899508969592603E-4	0.0
18	0.0	0.0	0.0	0.0011124386211990754	0.0
19	0.0	0.0	0.0	0.0013349263454388903	0.0
20	0.0	0.0	0.0	0.0020023895181583355	0.0
21	0.0	0.0	0.0	0.0026698526908777807	0.0
22	0.0	0.0	0.0	0.005339705381755561	0.0
23	0.0	0.0	0.0	0.009566972142312048	0.0
24	2.2248772423981506E-4	0.0	0.0	0.015129165248307425	0.0
25	2.2248772423981506E-4	0.0	0.0	0.018466481111904652	0.0
26	2.2248772423981506E-4	0.0	0.0	0.02491862511485929	0.0
27	2.2248772423981506E-4	0.0	0.0	0.036265499051089854	0.0
28	2.2248772423981506E-4	0.0	0.0	0.05339705381755561	0.0
29	2.2248772423981506E-4	0.0	0.0	0.07453338762033805	0.0
30	2.2248772423981506E-4	0.0	0.0	0.11280127618958624	0.0
31	2.2248772423981506E-4	0.0	0.0	0.182884909325128	0.0
32	2.2248772423981506E-4	0.0	0.0	0.2643154163969003	0.0
33	2.2248772423981506E-4	0.0	0.0	0.3377363653960393	0.0
34	2.2248772423981506E-4	0.0	0.0	0.4358534517857977	0.0
35	2.2248772423981506E-4	0.0	0.0	0.5488772156996238	0.0
36	2.2248772423981506E-4	0.0	0.0	0.7201927633642814	0.0
37	2.2248772423981506E-4	0.0	0.0	0.9555847756100058	0.0
38	2.2248772423981506E-4	0.0	0.0	1.2170078515917884	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTATA	60	1.9826984E-10	36.66639	44
TGCGATT	25	0.0023513136	35.19973	44
CGGACGG	30	0.0057414365	29.33311	28
TTCGTAC	70	3.5804987E-8	28.285498	34
CGCGGTA	85	8.20728E-9	25.882156	43
AATTCGT	80	1.3061072E-7	24.749813	32
TCGTACA	80	1.3061072E-7	24.749813	35
GTCCATA	45	0.0013951008	24.4497	1
ACTAGAT	45	0.001396911	24.44426	3
CTCGTTA	55	1.5915291E-4	23.999817	13
CCGTTTC	65	1.810212E-5	23.692127	23
TGGTATA	375	0.0	23.466488	44
CGTACAT	85	2.3460052E-7	23.29394	36
AAATTCG	90	4.0703162E-7	21.999834	31
TAGGAGC	90	4.0703162E-7	21.999834	44
TCCGGTC	120	5.9480953E-10	21.999834	23
AATCCCG	70	3.209751E-5	21.999832	19
ATCTAGG	100	4.608046E-8	21.999832	41
TATGCGT	200	0.0	21.999832	39
CCCGTTT	70	3.209751E-5	21.999832	22
>>END_MODULE