FastQCFastQC Report
Thu 2 Feb 2017
SRR4062468_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062468_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2117776
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG65660.3100422329840361No Hit
TCCATGTACTCTGCGTTGATACCAC56490.26674209170374963No Hit
GTATCAACGCAGAGTACTTTTTTTT49580.23411352286549664No Hit
TATCAACGCAGAGTACTTTTTTTTT35690.1685258497593702No Hit
GTACTTTTTTTTTTTTTTTTTTTTT32100.15157410415454703No Hit
GAGTACATGGAAGCAGTGGTATCAA29650.14000536411782927No Hit
GCGCAAGACGGACCAGAGCGAAAGC28020.13230861054238033No Hit
GTCTTGCGCCGGTCCAAGAATTTCA28010.13226139119529168No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG27590.13027817861756863No Hit
GAATAGGACCGCGGTTCTATTTTGT26200.12371468937224712No Hit
GATTAAGAGGGACGGCCGGGGGCAT25610.12092874789401713No Hit
CATGTACTCTGCGTTGATACCACTG25230.11913441270464867No Hit
GTATCTGATCGTCTTCGAACCTCCG25210.11903997401047137No Hit
GAATAACGCCGCCGCATCGCCAGTC24930.11771783229198934No Hit
GTCCTATTCCATTATTCCTAGCTGC24830.1172456388211029No Hit
GAACTACGACGGTATCTGATCGTCT23820.11247648476514986No Hit
CTATTGGAGCTGGAATTACCGCGGC23150.10931278851021073No Hit
GAGTACTTTTTTTTTTTTTTTTTTT22710.10723513723831038No Hit
GGGTAGGCACACGCTGAGCCAGTCA21580.10189935101729361No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATAA755.9031663E-815.1885241
ACTCGCA400.005277408814.2510298
TAGGACG759.697978E-713.9307154
AACCGCG2650.013.6236257
CGAACGA1800.012.66937916
CGCATCG4150.012.36292413
ATAATAC1405.2750693E-1112.2093933
ACGCGCG1251.4097168E-912.16145213
CGGACAT2050.012.0476555
CGCGGTC2850.012.0011510
ACTCTAA5150.011.9914410
GGTCGGC2800.011.87641911
CGGTCCA5150.011.80695610
TCGCCAG4200.011.76497917
GCATCGC4500.011.61249814
GTCTTAA907.5270273E-611.60234451
GTCCTAA1157.152266E-811.5564861
GCGTTAT2300.011.5564861
CGCCAGT4300.011.49137618
TCGGCGT2650.011.47306913
Produced by FastQC (version 0.11.5)