##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062468_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2117776
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.119370509440092	32.0	32.0	32.0	32.0	32.0
2	30.720736754028756	32.0	32.0	32.0	32.0	32.0
3	30.711329715701755	32.0	32.0	32.0	32.0	32.0
4	30.795965673423442	32.0	32.0	32.0	32.0	32.0
5	30.557623185832686	32.0	32.0	32.0	32.0	32.0
6	34.201569476658534	36.0	36.0	36.0	32.0	36.0
7	34.06744433783365	36.0	36.0	36.0	32.0	36.0
8	34.062037722592	36.0	36.0	36.0	32.0	36.0
9	34.24895125830116	36.0	36.0	36.0	32.0	36.0
10	33.87971768496762	36.0	36.0	36.0	32.0	36.0
11	34.237197418423854	36.0	36.0	36.0	32.0	36.0
12	33.977007955515596	36.0	36.0	36.0	32.0	36.0
13	34.13159559840134	36.0	36.0	36.0	32.0	36.0
14	34.01869602828628	36.0	36.0	36.0	32.0	36.0
15	33.94479350035131	36.0	36.0	36.0	32.0	36.0
16	33.94405640634326	36.0	36.0	36.0	32.0	36.0
17	33.85343728515197	36.0	36.0	36.0	32.0	36.0
18	33.88444481380467	36.0	36.0	36.0	32.0	36.0
19	33.87277880191295	36.0	36.0	36.0	32.0	36.0
20	33.86811778016183	36.0	36.0	36.0	32.0	36.0
21	33.835578928083045	36.0	36.0	36.0	32.0	36.0
22	33.779086645613134	36.0	36.0	36.0	32.0	36.0
23	33.744897477353604	36.0	36.0	36.0	32.0	36.0
24	33.71751308920301	36.0	36.0	36.0	27.0	36.0
25	33.25032534130144	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	14.0
4	80.0
5	221.0
6	384.0
7	106.0
8	317.0
9	420.0
10	240.0
11	79.0
12	157.0
13	120.0
14	418.0
15	513.0
16	754.0
17	1029.0
18	1389.0
19	2041.0
20	3227.0
21	4607.0
22	7116.0
23	10603.0
24	14541.0
25	20654.0
26	28138.0
27	36346.0
28	48025.0
29	63722.0
30	81189.0
31	110793.0
32	157044.0
33	224115.0
34	481500.0
35	817874.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.604313611412294	17.41661220187611	11.182030774810443	25.797043411901154
2	16.834106428884102	20.115532255391166	36.68718128402018	26.36318003170455
3	18.831469948792343	24.018630067640952	28.497033506921817	28.65286647664489
4	12.499710739020479	15.236757160094735	35.8092813812743	36.45425071961048
5	14.601998780344367	36.472993980607384	33.43117660868658	15.49383063036168
6	34.16234837150393	35.26842088106921	16.953007153827365	13.616223593599491
7	30.130080985797363	30.705047542948837	20.86630562723474	18.29856584401906
8	28.356767083068856	32.13959768846449	19.798017130922577	19.705618097544075
9	27.25179683371572	14.512404131843118	18.918628932922303	39.31717010151886
10	16.058357582018072	26.25079482894336	31.22863417067134	26.462213418367224
11	37.0564488105701	21.41607329518673	22.133697811006474	19.39378008323669
12	24.46385333385659	24.0674851876141	28.418882121730366	23.049779356798947
13	29.06531009767675	19.32458371549874	25.6159875942018	25.994118592622712
14	23.63659487714839	19.981021922657952	24.881800714112806	31.50058248608085
15	25.28973198816064	26.905470905779133	22.722835395356817	25.08196171070341
16	26.079988805613834	25.87255229168183	23.625260359298196	24.422198543406136
17	24.018680807446604	25.80846782581726	25.456571566895747	24.716279799840386
18	25.13881927961494	24.43228777577093	26.585877880641895	23.843015063972235
19	25.267452119616564	25.62561526044489	25.389298510892417	23.717634109046138
20	25.33297654460836	24.18649334046911	25.472020136500056	25.008509978422477
21	26.679971687822785	24.487858340768355	24.23603693821865	24.596133033190213
22	25.38814478379756	24.565651444801865	24.899387386228533	25.146816385172045
23	23.811617886824717	24.33540066048549	25.52893690585623	26.324044546833562
24	24.257075092534627	25.64812065342591	25.11868263223893	24.976121621800534
25	24.788769287361713	24.585127277438243	25.117282100212485	25.50882133498756
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	351.0
1	351.0
2	830.5
3	1310.0
4	1310.0
5	1310.0
6	3465.0
7	5620.0
8	5620.0
9	5620.0
10	5872.0
11	6124.0
12	6124.0
13	6124.0
14	6584.0
15	7044.0
16	7044.0
17	7044.0
18	11358.0
19	15672.0
20	15672.0
21	15672.0
22	24384.5
23	33097.0
24	33097.0
25	33097.0
26	48794.0
27	64491.0
28	64491.0
29	64491.0
30	77339.5
31	90188.0
32	90188.0
33	90188.0
34	111147.0
35	132106.0
36	132106.0
37	132106.0
38	148802.5
39	165499.0
40	165499.0
41	165499.0
42	190041.5
43	214584.0
44	214584.0
45	214584.0
46	254102.0
47	293620.0
48	293620.0
49	293620.0
50	287618.0
51	281616.0
52	281616.0
53	281616.0
54	256674.0
55	231732.0
56	231732.0
57	231732.0
58	213734.5
59	195737.0
60	195737.0
61	195737.0
62	175217.5
63	154698.0
64	154698.0
65	154698.0
66	129142.5
67	103587.0
68	103587.0
69	103587.0
70	78650.0
71	53713.0
72	53713.0
73	53713.0
74	42470.0
75	31227.0
76	31227.0
77	31227.0
78	25810.0
79	20393.0
80	20393.0
81	20393.0
82	14516.0
83	8639.0
84	8639.0
85	8639.0
86	6380.0
87	4121.0
88	4121.0
89	4121.0
90	2764.0
91	1407.0
92	1407.0
93	1407.0
94	847.0
95	287.0
96	287.0
97	287.0
98	600.0
99	913.0
100	913.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009443869417728786
2	0.004674715361775749
3	0.005855199038991848
4	0.014685216944568263
5	0.03706718746458549
6	0.04754988251826444
7	0.06983741434410438
8	0.09221938486412161
9	0.09963282235703871
10	0.11998436095224424
11	0.12291196047174016
12	0.13330021683124182
13	0.12447019892566542
14	0.1276811145276932
15	0.11611237449097543
16	0.11474301342540476
17	0.12579234064414743
18	0.13202529445984842
19	0.13306412009579863
20	0.12333693459553796
21	0.13150588164187335
22	0.1525184910963199
23	0.14014702215909522
24	0.13655835178035827
25	0.13174197837731658
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2117776.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.0978005047579
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.30920236422523	36.0988198827277
2	12.67089105040454	11.682004157181414
3	3.834194102315231	5.302437444751405
4	1.5742697741978446	2.9028149596657005
5	0.8522085384935321	1.9642469597963068
6	0.5156147926427905	1.4261224709129683
7	0.35596106851807263	1.1486315626804597
8	0.26849420791513057	0.9901593946523743
9	0.20683789469973837	0.8581294806023395
>10	1.1708980624156282	9.9364785321919
>50	0.10623605181489067	3.4684701775654943
>100	0.10714860858675564	10.824843299072144
>500	0.01920927276592264	6.145595666088665
>1k	0.00862876423709894	6.672826423406561
>5k	2.0544676754997475E-4	0.5784195887046973
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	6566	0.3100422329840361	No Hit
TCCATGTACTCTGCGTTGATACCAC	5649	0.26674209170374963	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4958	0.23411352286549664	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3569	0.1685258497593702	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3210	0.15157410415454703	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2965	0.14000536411782927	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2802	0.13230861054238033	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2801	0.13226139119529168	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2759	0.13027817861756863	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2620	0.12371468937224712	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2561	0.12092874789401713	No Hit
CATGTACTCTGCGTTGATACCACTG	2523	0.11913441270464867	No Hit
GTATCTGATCGTCTTCGAACCTCCG	2521	0.11903997401047137	No Hit
GAATAACGCCGCCGCATCGCCAGTC	2493	0.11771783229198934	No Hit
GTCCTATTCCATTATTCCTAGCTGC	2483	0.1172456388211029	No Hit
GAACTACGACGGTATCTGATCGTCT	2382	0.11247648476514986	No Hit
CTATTGGAGCTGGAATTACCGCGGC	2315	0.10931278851021073	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2271	0.10723513723831038	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	2158	0.10189935101729361	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8887738835457574E-4
2	0.0	0.0	0.0	0.0	1.8887738835457574E-4
3	0.0	0.0	0.0	0.0	1.8887738835457574E-4
4	0.0	0.0	0.0	0.0	1.8887738835457574E-4
5	0.0	0.0	0.0	0.0	2.833160825318636E-4
6	0.0	0.0	0.0	0.0	4.249741237977954E-4
7	0.0	0.0	0.0	0.0	4.249741237977954E-4
8	0.0	0.0	0.0	0.0	4.249741237977954E-4
9	0.0	0.0	0.0	4.7219347088643935E-5	4.249741237977954E-4
10	0.0	0.0	0.0	4.7219347088643935E-5	4.7219347088643936E-4
11	0.0	0.0	0.0	4.7219347088643935E-5	4.7219347088643936E-4
12	0.0	0.0	0.0	9.443869417728787E-5	4.7219347088643936E-4
13	0.0	0.0	0.0	9.443869417728787E-5	5.194128179750833E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATAA	75	5.9031663E-8	15.188524	1
ACTCGCA	40	0.0052774088	14.251029	8
TAGGACG	75	9.697978E-7	13.930715	4
AACCGCG	265	0.0	13.623625	7
CGAACGA	180	0.0	12.669379	16
CGCATCG	415	0.0	12.362924	13
ATAATAC	140	5.2750693E-11	12.209393	3
ACGCGCG	125	1.4097168E-9	12.161452	13
CGGACAT	205	0.0	12.047655	5
CGCGGTC	285	0.0	12.00115	10
ACTCTAA	515	0.0	11.99144	10
GGTCGGC	280	0.0	11.876419	11
CGGTCCA	515	0.0	11.806956	10
TCGCCAG	420	0.0	11.764979	17
GCATCGC	450	0.0	11.612498	14
GTCTTAA	90	7.5270273E-6	11.6023445	1
GTCCTAA	115	7.152266E-8	11.556486	1
GCGTTAT	230	0.0	11.556486	1
CGCCAGT	430	0.0	11.491376	18
TCGGCGT	265	0.0	11.473069	13
>>END_MODULE