FastQCFastQC Report
Thu 2 Feb 2017
SRR4062468_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062468_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2117776
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA31450.14850484659378518No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC28850.13622781635073775No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT27460.12966432710541625No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC27390.12933379167579573No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA26370.12451741827275405No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT25830.12196757352996729No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA25560.12069265115857389No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG23730.11205151064135206No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA23250.10978498198109714No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA22970.10846284026261512No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC22880.10803786613881733No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG21850.10317427338868701No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC21650.10222988644691412No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT21420.10114384146387531No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG3350.019.04467435
TAACGGC3400.018.76460536
TACGGTA600.00741412718.3332354
TAGATCG600.00741412718.3332355
CGATTAA1456.315531E-918.20679933
ACGATTA1456.315531E-918.20679932
TACGCAA851.4315346E-418.1175513
TAGTACG1355.5286364E-817.9266762
TACCGTC6450.017.7363387
ATACCGT6650.017.5337416
CGCGCCA1854.2382453E-1016.64855810
ACCGTCG6350.016.6298338
TAGGACG1206.4114374E-616.499914
CGCAATA8450.016.40227936
ACTAGAC1351.0864496E-616.296213
CGAATGC8400.016.23800843
CGAGCCG9700.016.10300615
CTATAGA4250.016.0500031
ATACGAA8500.016.04697240
CCGTCGT6650.015.5487899
Produced by FastQC (version 0.11.5)