##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062468_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2117776
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.266701483065255	32.0	32.0	32.0	32.0	32.0
2	31.403214504272405	32.0	32.0	32.0	32.0	32.0
3	31.484231571233217	32.0	32.0	32.0	32.0	32.0
4	31.58865526854587	32.0	32.0	32.0	32.0	32.0
5	31.521777562877283	32.0	32.0	32.0	32.0	32.0
6	35.100201815489456	36.0	36.0	36.0	36.0	36.0
7	35.11505182795536	36.0	36.0	36.0	36.0	36.0
8	35.060404877569674	36.0	36.0	36.0	36.0	36.0
9	35.1808047687763	36.0	36.0	36.0	36.0	36.0
10	35.05743430844433	36.0	36.0	36.0	36.0	36.0
11	35.18976888962761	36.0	36.0	36.0	36.0	36.0
12	35.093154800129945	36.0	36.0	36.0	36.0	36.0
13	35.14366533571067	36.0	36.0	36.0	36.0	36.0
14	35.09955443824087	36.0	36.0	36.0	36.0	36.0
15	35.06748872401992	36.0	36.0	36.0	36.0	36.0
16	35.0728542584296	36.0	36.0	36.0	36.0	36.0
17	35.043096625894336	36.0	36.0	36.0	36.0	36.0
18	35.06058997741027	36.0	36.0	36.0	36.0	36.0
19	35.03875952886424	36.0	36.0	36.0	36.0	36.0
20	35.02645841675418	36.0	36.0	36.0	36.0	36.0
21	35.0248052674126	36.0	36.0	36.0	36.0	36.0
22	35.002416686184	36.0	36.0	36.0	36.0	36.0
23	34.95730615513633	36.0	36.0	36.0	32.0	36.0
24	34.9311258603365	36.0	36.0	36.0	32.0	36.0
25	34.9015018585535	36.0	36.0	36.0	32.0	36.0
26	34.858234770816175	36.0	36.0	36.0	32.0	36.0
27	34.84609750984051	36.0	36.0	36.0	32.0	36.0
28	34.824087155582085	36.0	36.0	36.0	32.0	36.0
29	34.79365145322262	36.0	36.0	36.0	32.0	36.0
30	34.785703964914134	36.0	36.0	36.0	32.0	36.0
31	34.78076529340213	36.0	36.0	36.0	32.0	36.0
32	34.73709070269943	36.0	36.0	36.0	32.0	36.0
33	34.71228118554559	36.0	36.0	36.0	32.0	36.0
34	34.694998904511145	36.0	36.0	36.0	32.0	36.0
35	34.653932710541625	36.0	36.0	36.0	32.0	36.0
36	34.6295510006724	36.0	36.0	36.0	32.0	36.0
37	34.61412396778507	36.0	36.0	36.0	32.0	36.0
38	34.57022791834453	36.0	36.0	36.0	32.0	36.0
39	34.54286477890013	36.0	36.0	36.0	32.0	36.0
40	34.518224307008865	36.0	36.0	36.0	32.0	36.0
41	34.486779527202124	36.0	36.0	36.0	32.0	36.0
42	34.43706416542637	36.0	36.0	36.0	32.0	36.0
43	34.44276495720038	36.0	36.0	36.0	32.0	36.0
44	34.40640133800742	36.0	36.0	36.0	32.0	36.0
45	34.33561245381948	36.0	36.0	36.0	32.0	36.0
46	34.365519299491545	36.0	36.0	36.0	32.0	36.0
47	34.31087140471891	36.0	36.0	36.0	32.0	36.0
48	34.277041575690724	36.0	36.0	36.0	32.0	36.0
49	34.28405270434644	36.0	36.0	36.0	32.0	36.0
50	33.74059532264035	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	14.0
21	60.0
22	196.0
23	554.0
24	1545.0
25	3657.0
26	7670.0
27	14249.0
28	24173.0
29	36199.0
30	50977.0
31	72311.0
32	106063.0
33	169424.0
34	366304.0
35	1264375.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.924915417208986	18.056229846060265	11.733016852330065	26.285837884400685
2	16.173062320795133	20.603743327628997	36.40819610820857	26.814998243367295
3	18.254742506476177	24.23827606690459	28.895787156441564	28.61119427017767
4	12.211223771386264	15.650733105011728	36.05712226947836	36.08092085412365
5	14.330788525320903	36.65774850597986	33.35900491836719	15.652458050332047
6	34.41603238889338	35.40745588623467	16.648557935897454	13.527953788974498
7	30.12172214787791	30.678863750334077	20.915923984334494	18.283490117453518
8	28.391718482030203	32.29184767416384	19.700808772976934	19.615625070829022
9	27.32814990820559	14.201029759521308	18.894113447314542	39.57670688495856
10	15.968686017784695	26.258915012730338	31.370314896381863	26.402084073103104
11	37.41382469156322	21.140007252891714	22.091807632157508	19.354360423387554
12	24.4411590272059	24.043194369942807	28.408056376122875	23.107590226728416
13	29.28548628372406	19.149334018328663	25.679958598076475	25.885221099870808
14	23.824663231616565	19.729659794048096	24.8657081768799	31.579968797455443
15	25.441170359849202	26.82176963002697	22.625480692953364	25.111579317170467
16	26.248290659635394	25.717120224235234	23.584411193629542	24.45017792249983
17	24.071573197543085	25.763725719811724	25.366894326878764	24.797806755766427
18	25.37605488021396	24.251809445380438	26.542750508080175	23.82938516632543
19	25.45014203579604	25.450236474490218	25.36698876557294	23.7326327241408
20	25.375252151313454	24.040408428464577	25.567529332658413	25.016810087563556
21	26.871208286428782	24.331940677389866	24.286610104184767	24.510240931996584
22	25.441075921155022	24.392098125580798	25.02823716955901	25.138588783705167
23	23.95701906150603	24.046074750115213	25.57739817619994	26.419508012178817
24	24.388509455202062	25.484139965699864	25.108746156345145	25.018604422752926
25	24.94536721541844	24.263378185417157	25.2394965284336	25.551758070730806
26	24.298131624874397	25.16583434697532	25.86911930251358	24.666914725636705
27	25.622728749405038	24.725655593414977	24.8968729459584	24.754742711221585
28	24.377649005371673	24.279338324733114	26.013610504604827	25.329402165290382
29	24.21299514207357	24.968882450268584	25.99240901776203	24.825713389895814
30	24.641369058861752	24.81088651490998	26.09468612355603	24.453058302672236
31	24.805881264118586	24.769333489471972	24.863819402996352	25.56096584341309
32	24.513499066945702	24.81886658456796	24.952827872258446	25.714806476227892
33	24.160487228111	24.354275428562794	25.544722388014595	25.94051495531161
34	24.862922235401665	24.622056345902493	25.95855274589947	24.556468672796367
35	25.960441519783018	24.33765421838759	25.500241763057097	24.201662498772297
36	24.483562000891503	25.090897243145637	25.162009579861138	25.263531176101722
37	25.771658570122618	24.857539229833563	24.99447533639063	24.37632686365319
38	24.52299015571052	24.62923368665997	25.464827252740612	25.382948904888902
39	25.010341037012413	24.636080491987823	24.906647350805752	25.446931120194016
40	25.355278367494954	24.788835079819584	25.365005552995218	24.49088099969024
41	23.98119536721542	25.212534281245986	25.978857065147587	24.827413286391007
42	25.9097751603569	25.213950861658645	25.212534281245986	23.663739696738464
43	24.81782775893201	23.878493287297616	25.42431305293855	25.879365900831814
44	24.4066416844841	24.662192790927843	25.57668988599361	25.354475638594447
45	24.543058378223193	24.862875016054577	25.51912950189255	25.074937103829676
46	24.144691548767717	24.60068921424099	25.85663059419938	25.397988642791912
47	24.56086007207561	24.347853597358736	26.162351447934057	24.92893488263159
48	25.32874109443114	25.667776006527603	24.6744226018238	24.32906029721746
49	23.92585429242753	25.16361503766215	25.720944991349416	25.189585678560906
50	23.922560234208074	25.845332955578375	24.9470788917614	25.285027918452148
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	22.0
1	33.0
2	44.0
3	178.0
4	312.0
5	364.0
6	416.0
7	410.5
8	405.0
9	526.5
10	648.0
11	955.0
12	1262.0
13	2119.0
14	2976.0
15	4108.5
16	5241.0
17	6175.5
18	7110.0
19	7718.0
20	8326.0
21	9593.5
22	10861.0
23	11494.5
24	12128.0
25	13917.0
26	15706.0
27	19359.5
28	23013.0
29	25693.5
30	28374.0
31	30312.5
32	32251.0
33	36816.0
34	41381.0
35	47271.0
36	53161.0
37	65225.0
38	77289.0
39	84498.0
40	91707.0
41	100631.5
42	109556.0
43	112246.5
44	114937.0
45	129613.5
46	144290.0
47	155396.0
48	166502.0
49	167425.0
50	168348.0
51	156858.5
52	145369.0
53	141981.0
54	138593.0
55	141249.0
56	143905.0
57	141038.0
58	138171.0
59	126275.0
60	114379.0
61	100784.5
62	87190.0
63	76861.5
64	66533.0
65	56448.0
66	46363.0
67	41401.0
68	36439.0
69	34486.0
70	32533.0
71	24977.0
72	17421.0
73	15629.0
74	13837.0
75	9984.5
76	6132.0
77	5711.0
78	5290.0
79	4543.0
80	3796.0
81	2996.5
82	2197.0
83	1898.5
84	1600.0
85	1345.5
86	1091.0
87	780.5
88	470.0
89	296.0
90	122.0
91	78.5
92	35.0
93	26.0
94	17.0
95	13.5
96	10.0
97	10.0
98	10.0
99	8.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015299068456720634
2	0.004155302543800666
3	6.610708592410151E-4
4	1.4165804126593182E-4
5	0.0
6	6.138515121523712E-4
7	9.443869417728787E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	9.443869417728787E-5
47	0.0
48	0.0
49	0.0
50	4.7219347088643935E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2117776.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.49448527013298
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.77233683655766	38.98796265250956
2	12.662471493362942	12.534450176234618
3	3.7098857375383383	5.508566549714032
4	1.4950741939157457	2.9599171067407526
5	0.8030319518012943	1.9872826554937648
6	0.5035032223654499	1.4952379693698747
7	0.3373627424470791	1.1688316700717034
8	0.23769521518401085	0.9411681861364891
9	0.19224534082057676	0.8563575770546157
>10	1.0750869961811653	9.76699650488962
>50	0.09398206574669592	3.258681390369955
>100	0.09518440169357344	10.185184942002463
>500	0.015334928059349424	5.119084972034075
>1k	0.006804874326336307	5.230277647378546
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	3145	0.14850484659378518	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2885	0.13622781635073775	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2746	0.12966432710541625	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2739	0.12933379167579573	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2637	0.12451741827275405	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2583	0.12196757352996729	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2556	0.12069265115857389	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	2373	0.11205151064135206	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	2325	0.10978498198109714	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	2297	0.10846284026261512	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	2288	0.10803786613881733	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	2185	0.10317427338868701	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	2165	0.10222988644691412	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	2142	0.10114384146387531	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.416580412659318E-4
2	0.0	0.0	0.0	0.0	1.416580412659318E-4
3	0.0	0.0	0.0	0.0	1.416580412659318E-4
4	0.0	0.0	0.0	0.0	1.416580412659318E-4
5	0.0	0.0	0.0	0.0	1.416580412659318E-4
6	0.0	0.0	0.0	0.0	1.416580412659318E-4
7	0.0	0.0	0.0	0.0	1.416580412659318E-4
8	0.0	0.0	0.0	0.0	1.416580412659318E-4
9	0.0	0.0	0.0	0.0	1.416580412659318E-4
10	0.0	0.0	0.0	0.0	1.416580412659318E-4
11	0.0	0.0	0.0	0.0	1.416580412659318E-4
12	0.0	0.0	0.0	0.0	4.7219347088643936E-4
13	0.0	0.0	0.0	0.0	5.194128179750833E-4
14	0.0	0.0	0.0	0.0	5.194128179750833E-4
15	0.0	0.0	0.0	0.0	5.194128179750833E-4
16	0.0	0.0	0.0	9.443869417728787E-5	5.666321650637272E-4
17	0.0	0.0	0.0	9.443869417728787E-5	6.610708592410151E-4
18	0.0	0.0	0.0	2.833160825318636E-4	7.55509553418303E-4
19	0.0	0.0	0.0	4.249741237977954E-4	7.55509553418303E-4
20	0.0	0.0	0.0	5.666321650637272E-4	8.02728900506947E-4
21	0.0	0.0	0.0	7.55509553418303E-4	8.02728900506947E-4
22	0.0	0.0	0.0	0.0012749223713933863	8.499482475955908E-4
23	0.0	0.0	0.0	0.0022665286602549088	9.916062888615227E-4
24	0.0	0.0	0.0	0.0035414510316482952	9.916062888615227E-4
25	0.0	0.0	0.0	0.004580276667598462	0.0010388256359501666
26	0.0	0.0	0.0	0.005383005568105409	0.0010388256359501666
27	0.0	0.0	0.0	0.007224560104562522	0.0010860449830388105
28	0.0	0.0	0.0	0.015157410415454703	0.0010860449830388105
29	0.0	0.0	0.0	0.029559311277491104	0.0010860449830388105
30	0.0	0.0	0.0	0.056804874547638655	0.0010860449830388105
31	0.0	0.0	0.0	0.11738729686236883	0.0011332643301274544
32	0.0	0.0	0.0	0.18410823429862272	0.0011332643301274544
33	0.0	0.0	0.0	0.25163190063538354	0.0011332643301274544
34	0.0	0.0	0.0	0.32760783010101163	0.0011804836772160985
35	0.0	0.0	0.0	0.4183634152053853	0.0011804836772160985
36	0.0	0.0	0.0	0.548358277740422	0.0011804836772160985
37	0.0	0.0	0.0	0.7288778416603078	0.0011804836772160985
38	0.0	0.0	0.0	0.9683271507468212	0.0012277030243047424
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAACGG	335	0.0	19.044674	35
TAACGGC	340	0.0	18.764605	36
TACGGTA	60	0.007414127	18.333235	4
TAGATCG	60	0.007414127	18.333235	5
CGATTAA	145	6.315531E-9	18.206799	33
ACGATTA	145	6.315531E-9	18.206799	32
TACGCAA	85	1.4315346E-4	18.11755	13
TAGTACG	135	5.5286364E-8	17.926676	2
TACCGTC	645	0.0	17.736338	7
ATACCGT	665	0.0	17.533741	6
CGCGCCA	185	4.2382453E-10	16.648558	10
ACCGTCG	635	0.0	16.629833	8
TAGGACG	120	6.4114374E-6	16.49991	4
CGCAATA	845	0.0	16.402279	36
ACTAGAC	135	1.0864496E-6	16.29621	3
CGAATGC	840	0.0	16.238008	43
CGAGCCG	970	0.0	16.103006	15
CTATAGA	425	0.0	16.050003	1
ATACGAA	850	0.0	16.046972	40
CCGTCGT	665	0.0	15.548789	9
>>END_MODULE