FastQCFastQC Report
Thu 2 Feb 2017
SRR4062467_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062467_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2127534
Sequences flagged as poor quality0
Sequence length25
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT164250.7720205646537258No Hit
GTCCTACAGTGGACATTTCTAAATT144690.6800831385068347No Hit
CTGTAGGACGTGGAATATGGCAAGA139560.6559707153916224No Hit
CTTTAGGACGTGAAATATGGCGAGG130010.6110830661225626No Hit
GTCCTACAGTGTGCATTTCTCATTT96650.4542818117125273No Hit
CTGTAGGACCTGGAATATGGCGAGA71130.33433073219981446No Hit
CTGAAGGACCTGGAATATGGCGAGA71050.3339547100069846No Hit
GTCCTTCAGTGTGCATTTCTCATTT64410.3027448680021095No Hit
ATTTAGAAATGTCCACTGTAGGACG62010.29146420221721486No Hit
TTTCTAAATTTTCCACCTTTTTCAG50390.23684697870868338No Hit
GAATATGGCAAGAAAACTGAAAATC45720.2148966832022426No Hit
GTATCAACGCAGAGTACTTTTTTTT41540.19524952362688447No Hit
GGAATATGGCGAGAAAACTGAAAAT39820.18716504648104332No Hit
GATATACACTGTTCTACAAATCCCG37450.17602538901845985No Hit
TTGTAGAACAGTGTATATCAATGAG35170.16530875652280996No Hit
GAAATATGGCGAGGAAAACTGAAAA33780.15877537092239186No Hit
ATTCCAGGTCCTTCAGTGTGCATTT33610.15797632376262846No Hit
CCATATTCCAGGTCCTTCAGTGTGC32940.1548271378976787No Hit
GCCATATTCCACGTCCTACAGTGGA32760.15398108796381163No Hit
ACCTGGAATATGGCGAGAAAACTGA32290.15177195758093642No Hit
CTGTAGGACATGGAATATGGCAAGA30140.14166636114863498No Hit
CCATATTTCACGTCCTAAAGTGTGT29990.14096131953707908No Hit
GACCTGGAATATGGCGAGAAAACTG29250.13748311425340323No Hit
TTCCAGGTCCTTCAGTGTGCATTTC29130.1369190809641585No Hit
GTACTTTTTTTTTTTTTTTTTTTTT27930.1312787480717112No Hit
GTGTATATCAATGAGTTACAATGAG27790.130620709234259No Hit
GTCCACTGTAGGACGTGGAATATGG27480.12916362323704345No Hit
GTGTATATCAATGAGTTACAATGAA26880.1263434567908198No Hit
ATACACACTTTAGGACGTGAAATAT26710.12554440963105643No Hit
ACAGTGGACATTTCTAAATTTTCCA26600.1250273791159154No Hit
TATCAACGCAGAGTACTTTTTTTTT26190.12310026537766258No Hit
CCTAAAGTGTGTATTTCTCATTTTC25640.12051511280195756No Hit
GTTCTACAGTGTGGTTTTTATCATT25550.12009208783502402No Hit
CACTTTAGGACGTGAAATATGGCGA25370.11924603790115693No Hit
CCACTGTAGGACGTGGAATATGGCA24620.11572082984337737No Hit
AGTGTGTATTTCTCATTTTCCGTGA23520.11055052469196731No Hit
GTGTATTTCTCATTTTCCGTGATTT23020.10820038598678093No Hit
GAAATACACACTTTAGGACGTGAAA22310.10486318902541629No Hit
CTACAGTGGACATTTCTAAATTTTC22130.1040171390915492No Hit
GTAGGACGTGGAATATGGCAAGAAA22000.10340610302820073No Hit
ATTCCACGTCCTACAGTGGACATTT21970.10326509470588954No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCACG350.002179892316.275251
CTCGAAT704.436206E-714.92916210
TCCAACG6300.014.78288818
GGCGTCC456.760534E-414.7787128
CCAACGA4400.014.68689419
CCAACGT2100.014.48119519
TAGGACC26200.013.9931154
TCGGCAA1205.0931703E-1113.458549513
TCCGTTC1352.7284841E-1112.66895816
CCACCTT15650.012.62640113
GTCCTAT4200.012.4324821
CACCTTT16050.012.43069214
CGGCAAA1301.9826984E-1012.42386214
AATCGTA1301.9826984E-1012.42327713
AAATGTC18000.012.4035627
GCGTTCA1155.326001E-912.39150413
TCGTCTT3250.012.277698514
CCATCCG550.003067631712.0916729
TAGCTCG1103.814239E-812.09138910
TAGAAAT18350.012.0600264
Produced by FastQC (version 0.11.5)