##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062467_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2127534
Sequences flagged as poor quality	0
Sequence length	25
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.231759398439696	32.0	32.0	32.0	32.0	32.0
2	30.998247736581412	32.0	32.0	32.0	32.0	32.0
3	30.98257748172297	32.0	32.0	32.0	32.0	32.0
4	31.019726124235852	32.0	32.0	32.0	32.0	32.0
5	30.956079197794253	32.0	32.0	32.0	32.0	32.0
6	34.605775982898514	36.0	36.0	36.0	32.0	36.0
7	34.471580712693665	36.0	36.0	36.0	32.0	36.0
8	34.4847471297756	36.0	36.0	36.0	32.0	36.0
9	34.547886896284616	36.0	36.0	36.0	32.0	36.0
10	34.32357649748488	36.0	36.0	36.0	32.0	36.0
11	34.56073698469684	36.0	36.0	36.0	32.0	36.0
12	34.3905418197782	36.0	36.0	36.0	32.0	36.0
13	34.466510053423356	36.0	36.0	36.0	32.0	36.0
14	34.41317741573108	36.0	36.0	36.0	32.0	36.0
15	34.391185757783425	36.0	36.0	36.0	32.0	36.0
16	34.41969999069345	36.0	36.0	36.0	32.0	36.0
17	34.39023677177427	36.0	36.0	36.0	32.0	36.0
18	34.412613852469576	36.0	36.0	36.0	32.0	36.0
19	34.31287913612661	36.0	36.0	36.0	32.0	36.0
20	34.297586783571965	36.0	36.0	36.0	32.0	36.0
21	34.25189397678251	36.0	36.0	36.0	32.0	36.0
22	34.215277875700224	36.0	36.0	36.0	32.0	36.0
23	34.22437949287767	36.0	36.0	36.0	32.0	36.0
24	34.17586369947554	36.0	36.0	36.0	32.0	36.0
25	33.782876325360725	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	12.0
4	85.0
5	201.0
6	363.0
7	91.0
8	311.0
9	449.0
10	262.0
11	85.0
12	166.0
13	119.0
14	373.0
15	452.0
16	670.0
17	970.0
18	1267.0
19	1715.0
20	2551.0
21	3643.0
22	5288.0
23	7701.0
24	10750.0
25	14857.0
26	20740.0
27	27152.0
28	36756.0
29	49150.0
30	64127.0
31	87906.0
32	125547.0
33	184466.0
34	440959.0
35	1038349.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.59423533343769	21.414029451817505	12.501815648563769	25.489919566181037
2	11.612699439332463	21.663915347098705	47.6528862533044	19.07049896026443
3	18.113297477797115	27.455526067651707	31.015931929007166	23.415244525544015
4	10.26822545850114	18.468759211039096	41.64830530983644	29.614710020623324
5	9.812499441640762	41.249699661505	37.91872828570313	11.019072611151104
6	27.488035543734394	40.97564691769178	20.098264430824823	11.438053107748997
7	25.02777451141789	33.341454810564194	24.202535217892336	17.428235460125585
8	21.179660863850145	43.81375033696733	21.287772684781594	13.718816114400937
9	26.289367003797903	15.595017182777324	22.405315933497192	35.71029987992758
10	14.310609586620352	30.09135963638742	37.029854124831346	18.56817665216088
11	32.02478055355284	21.59394453929894	30.000348252993565	16.38092665415466
12	24.790816396172588	24.98572651676646	34.92218892396529	15.301268163095669
13	29.15915630432757	24.275875570983633	27.30344185591211	19.26152626877669
14	17.770392985273347	26.857327346223407	28.64094142142248	26.731338247080767
15	19.13347275372849	39.880178146888355	23.370296679322426	17.616052420060726
16	17.317548226128345	29.29011722597352	35.11471072793419	18.277623819963946
17	17.470618355832748	32.571738651616435	31.94141931743094	18.016223675119868
18	17.511681891092017	28.153734063302483	38.24364979554337	16.090934250062126
19	22.894163485293987	26.910765143859543	28.758306830392634	21.436764540453833
20	22.445854237064786	30.444414115077983	30.94544479061756	16.16428685723967
21	19.95377123113396	27.999845621704463	27.859822624986407	24.18656052217517
22	21.47136445250822	33.941788305876436	28.15023034792753	16.436616893687816
23	17.88633921245862	33.18061635024141	31.277451752463865	17.65559268483611
24	22.32649430237648	28.852567813722306	32.11398071139101	16.706957172510204
25	19.505767597904196	31.403662194040553	32.09714830078489	16.993421907270356
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	392.0
1	392.0
2	946.5
3	1501.0
4	1501.0
5	1501.0
6	3917.5
7	6334.0
8	6334.0
9	6334.0
10	7001.0
11	7668.0
12	7668.0
13	7668.0
14	9969.5
15	12271.0
16	12271.0
17	12271.0
18	21798.5
19	31326.0
20	31326.0
21	31326.0
22	55626.5
23	79927.0
24	79927.0
25	79927.0
26	126466.0
27	173005.0
28	173005.0
29	173005.0
30	230118.5
31	287232.0
32	287232.0
33	287232.0
34	302745.0
35	318258.0
36	318258.0
37	318258.0
38	311213.0
39	304168.0
40	304168.0
41	304168.0
42	291606.0
43	279044.0
44	279044.0
45	279044.0
46	272227.0
47	265410.0
48	265410.0
49	265410.0
50	223750.5
51	182091.0
52	182091.0
53	182091.0
54	135080.5
55	88070.0
56	88070.0
57	88070.0
58	66762.5
59	45455.0
60	45455.0
61	45455.0
62	34155.5
63	22856.0
64	22856.0
65	22856.0
66	17170.5
67	11485.0
68	11485.0
69	11485.0
70	8454.5
71	5424.0
72	5424.0
73	5424.0
74	4052.5
75	2681.0
76	2681.0
77	2681.0
78	1953.5
79	1226.0
80	1226.0
81	1226.0
82	883.5
83	541.0
84	541.0
85	541.0
86	398.0
87	255.0
88	255.0
89	255.0
90	177.5
91	100.0
92	100.0
93	100.0
94	87.5
95	75.0
96	75.0
97	75.0
98	407.0
99	739.0
100	739.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009165540950226881
2	0.003290194187260932
3	0.005076299603202581
4	0.015557918228333837
5	0.03605112773755907
6	0.04827184900452825
7	0.06975211676993176
8	0.09198442892099491
9	0.10105596432301435
10	0.12385230976332223
11	0.12418132918204831
12	0.13936322521755234
13	0.1265314678872347
14	0.12911662046293973
15	0.11966906286809047
16	0.11717791584059292
17	0.12869359549600617
18	0.13527398387052803
19	0.1375771198016107
20	0.1275655289175167
21	0.13550899774104666
22	0.15774130989210983
23	0.14688366907414874
24	0.1393162224434486
25	0.1365900615454324
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2127534.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.050633350989926
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.95010524279492	36.01802877666211
2	12.69456045567678	11.437959772813407
3	3.317364821007186	4.4834815872800124
4	1.190040326357746	2.144482816625408
5	0.6208748359141494	1.398540229481219
6	0.383464349284871	1.0365187081685194
7	0.26225454655185937	0.8270313394936555
8	0.19093536501822783	0.6881407298538889
9	0.1403335971967806	0.5689905690723904
>10	0.9883442068620829	8.635969187845149
>50	0.12005684052480577	3.8420077246469906
>100	0.11458793171953283	10.811450820002523
>500	0.015786485114845292	4.858801437494038
>1k	0.010245533385793633	8.566273886217582
>5k	6.272775542322633E-4	1.9576125215486537
>10k+	4.181850361548421E-4	2.7247098927945137
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTT	16425	0.7720205646537258	No Hit
GTCCTACAGTGGACATTTCTAAATT	14469	0.6800831385068347	No Hit
CTGTAGGACGTGGAATATGGCAAGA	13956	0.6559707153916224	No Hit
CTTTAGGACGTGAAATATGGCGAGG	13001	0.6110830661225626	No Hit
GTCCTACAGTGTGCATTTCTCATTT	9665	0.4542818117125273	No Hit
CTGTAGGACCTGGAATATGGCGAGA	7113	0.33433073219981446	No Hit
CTGAAGGACCTGGAATATGGCGAGA	7105	0.3339547100069846	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	6441	0.3027448680021095	No Hit
ATTTAGAAATGTCCACTGTAGGACG	6201	0.29146420221721486	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	5039	0.23684697870868338	No Hit
GAATATGGCAAGAAAACTGAAAATC	4572	0.2148966832022426	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4154	0.19524952362688447	No Hit
GGAATATGGCGAGAAAACTGAAAAT	3982	0.18716504648104332	No Hit
GATATACACTGTTCTACAAATCCCG	3745	0.17602538901845985	No Hit
TTGTAGAACAGTGTATATCAATGAG	3517	0.16530875652280996	No Hit
GAAATATGGCGAGGAAAACTGAAAA	3378	0.15877537092239186	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	3361	0.15797632376262846	No Hit
CCATATTCCAGGTCCTTCAGTGTGC	3294	0.1548271378976787	No Hit
GCCATATTCCACGTCCTACAGTGGA	3276	0.15398108796381163	No Hit
ACCTGGAATATGGCGAGAAAACTGA	3229	0.15177195758093642	No Hit
CTGTAGGACATGGAATATGGCAAGA	3014	0.14166636114863498	No Hit
CCATATTTCACGTCCTAAAGTGTGT	2999	0.14096131953707908	No Hit
GACCTGGAATATGGCGAGAAAACTG	2925	0.13748311425340323	No Hit
TTCCAGGTCCTTCAGTGTGCATTTC	2913	0.1369190809641585	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2793	0.1312787480717112	No Hit
GTGTATATCAATGAGTTACAATGAG	2779	0.130620709234259	No Hit
GTCCACTGTAGGACGTGGAATATGG	2748	0.12916362323704345	No Hit
GTGTATATCAATGAGTTACAATGAA	2688	0.1263434567908198	No Hit
ATACACACTTTAGGACGTGAAATAT	2671	0.12554440963105643	No Hit
ACAGTGGACATTTCTAAATTTTCCA	2660	0.1250273791159154	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2619	0.12310026537766258	No Hit
CCTAAAGTGTGTATTTCTCATTTTC	2564	0.12051511280195756	No Hit
GTTCTACAGTGTGGTTTTTATCATT	2555	0.12009208783502402	No Hit
CACTTTAGGACGTGAAATATGGCGA	2537	0.11924603790115693	No Hit
CCACTGTAGGACGTGGAATATGGCA	2462	0.11572082984337737	No Hit
AGTGTGTATTTCTCATTTTCCGTGA	2352	0.11055052469196731	No Hit
GTGTATTTCTCATTTTCCGTGATTT	2302	0.10820038598678093	No Hit
GAAATACACACTTTAGGACGTGAAA	2231	0.10486318902541629	No Hit
CTACAGTGGACATTTCTAAATTTTC	2213	0.1040171390915492	No Hit
GTAGGACGTGGAATATGGCAAGAAA	2200	0.10340610302820073	No Hit
ATTCCACGTCCTACAGTGGACATTT	2197	0.10326509470588954	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.70027741037276E-5	0.0
5	0.0	0.0	0.0	4.70027741037276E-5	0.0
6	0.0	0.0	0.0	4.70027741037276E-5	0.0
7	0.0	0.0	0.0	1.4100832231118282E-4	0.0
8	0.0	0.0	0.0	1.880110964149104E-4	0.0
9	0.0	0.0	0.0	1.880110964149104E-4	0.0
10	0.0	0.0	0.0	1.880110964149104E-4	0.0
11	0.0	0.0	0.0	1.880110964149104E-4	0.0
12	0.0	0.0	0.0	1.880110964149104E-4	0.0
13	0.0	0.0	0.0	1.880110964149104E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCCACG	35	0.0021798923	16.27525	1
CTCGAAT	70	4.436206E-7	14.929162	10
TCCAACG	630	0.0	14.782888	18
GGCGTCC	45	6.760534E-4	14.778712	8
CCAACGA	440	0.0	14.686894	19
CCAACGT	210	0.0	14.481195	19
TAGGACC	2620	0.0	13.993115	4
TCGGCAA	120	5.0931703E-11	13.4585495	13
TCCGTTC	135	2.7284841E-11	12.668958	16
CCACCTT	1565	0.0	12.626401	13
GTCCTAT	420	0.0	12.432482	1
CACCTTT	1605	0.0	12.430692	14
CGGCAAA	130	1.9826984E-10	12.423862	14
AATCGTA	130	1.9826984E-10	12.423277	13
AAATGTC	1800	0.0	12.403562	7
GCGTTCA	115	5.326001E-9	12.391504	13
TCGTCTT	325	0.0	12.2776985	14
CCATCCG	55	0.0030676317	12.091672	9
TAGCTCG	110	3.814239E-8	12.091389	10
TAGAAAT	1835	0.0	12.060026	4
>>END_MODULE