FastQCFastQC Report
Thu 2 Feb 2017
SRR4062467_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062467_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2127534
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA79260.37254398754614493No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA63580.2988436377515001No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA58040.272804100898035No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC57790.27162903154544177No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA40730.19144229892448253No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA32720.1537930768673967No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA25580.12023309615733521No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA24200.1137467133310208No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT23110.10862341095371449No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG21740.10218403090150381No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTCG250.002353255235.1998148
GCGACGA554.9635837E-627.9998520
TAGGACC30250.024.7271394
TGTCCGC901.5141268E-824.44431510
GTCCGCT852.3545181E-723.29399311
GGCGACG602.873875E-421.99988419
TGTAGGA95550.021.2520872
CTGTAGG92700.021.1737291
GGACGTC755.475366E-520.5332246
AGGACCT61750.020.4144675
GTAGGAC100600.020.097313
GTCCTAT4300.019.9576031
GGACCTG61450.019.9413096
TAGGACA22400.019.5445384
TTAGGAC57200.019.4229743
TAGGACG103950.019.3438134
GCGACAA808.993035E-519.24989720
CGACGAA808.993035E-519.24989721
GACGTGT1152.0233165E-719.1303337
AGGACGT103300.019.1247775
Produced by FastQC (version 0.11.5)