##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062467_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2127534
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.334648000925014	32.0	32.0	32.0	32.0	32.0
2	31.518920496687713	32.0	32.0	32.0	32.0	32.0
3	31.579944668334324	32.0	32.0	32.0	32.0	32.0
4	31.658251289991135	32.0	32.0	32.0	32.0	32.0
5	31.639581318089395	32.0	32.0	32.0	32.0	32.0
6	35.23670033005348	36.0	36.0	36.0	36.0	36.0
7	35.26308580732435	36.0	36.0	36.0	36.0	36.0
8	35.20213166981115	36.0	36.0	36.0	36.0	36.0
9	35.31984494724879	36.0	36.0	36.0	36.0	36.0
10	35.23796094445494	36.0	36.0	36.0	36.0	36.0
11	35.32554121344242	36.0	36.0	36.0	36.0	36.0
12	35.26213635128745	36.0	36.0	36.0	36.0	36.0
13	35.29026234128338	36.0	36.0	36.0	36.0	36.0
14	35.26224868791756	36.0	36.0	36.0	36.0	36.0
15	35.22852701766458	36.0	36.0	36.0	36.0	36.0
16	35.26330437022393	36.0	36.0	36.0	36.0	36.0
17	35.27181610258638	36.0	36.0	36.0	36.0	36.0
18	35.275227093903084	36.0	36.0	36.0	36.0	36.0
19	35.235725022490826	36.0	36.0	36.0	36.0	36.0
20	35.2134748492856	36.0	36.0	36.0	36.0	36.0
21	35.18585648924999	36.0	36.0	36.0	36.0	36.0
22	35.16965557307192	36.0	36.0	36.0	36.0	36.0
23	35.16237343328003	36.0	36.0	36.0	36.0	36.0
24	35.153721162623015	36.0	36.0	36.0	36.0	36.0
25	35.121573145247034	36.0	36.0	36.0	36.0	36.0
26	35.08733068425698	36.0	36.0	36.0	36.0	36.0
27	35.03664195260804	36.0	36.0	36.0	36.0	36.0
28	35.005421769992864	36.0	36.0	36.0	36.0	36.0
29	34.97744524881858	36.0	36.0	36.0	36.0	36.0
30	34.92903427160271	36.0	36.0	36.0	36.0	36.0
31	34.92676826786317	36.0	36.0	36.0	36.0	36.0
32	34.898656848727214	36.0	36.0	36.0	36.0	36.0
33	34.91999328800386	36.0	36.0	36.0	36.0	36.0
34	34.889558051716214	36.0	36.0	36.0	36.0	36.0
35	34.86749635963515	36.0	36.0	36.0	36.0	36.0
36	34.80521251364255	36.0	36.0	36.0	36.0	36.0
37	34.74956122910374	36.0	36.0	36.0	36.0	36.0
38	34.71377237684568	36.0	36.0	36.0	32.0	36.0
39	34.56872651623899	36.0	36.0	36.0	32.0	36.0
40	34.632671910296146	36.0	36.0	36.0	32.0	36.0
41	34.59704098735907	36.0	36.0	36.0	32.0	36.0
42	34.6057816232314	36.0	36.0	36.0	32.0	36.0
43	34.583809706448875	36.0	36.0	36.0	32.0	36.0
44	34.506980381982146	36.0	36.0	36.0	32.0	36.0
45	34.48186397961208	36.0	36.0	36.0	32.0	36.0
46	34.36487830511757	36.0	36.0	36.0	32.0	36.0
47	34.3802792340804	36.0	36.0	36.0	32.0	36.0
48	34.314872053748616	36.0	36.0	36.0	32.0	36.0
49	34.26748291684175	36.0	36.0	36.0	32.0	36.0
50	33.85169684714792	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	4.0
20	15.0
21	32.0
22	117.0
23	434.0
24	1019.0
25	2701.0
26	5896.0
27	11611.0
28	20673.0
29	31713.0
30	45483.0
31	64672.0
32	94297.0
33	150886.0
34	321487.0
35	1376493.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.06159725346455	22.124550286786928	12.947885683876702	25.86596677587182
2	11.303728174287942	22.190425782942924	47.0692697546608	19.43657628810833
3	17.770336677911356	27.777890062763127	31.46701586774855	22.984757391576967
4	10.19586092793051	18.92838173844787	41.9454484778123	28.930308855809315
5	9.5876303681306	41.575148305572704	37.71532568472498	11.121895641571717
6	27.77455806505136	41.078364047512615	19.7532900998769	11.393787787559125
7	25.073641630940624	33.459786522700234	24.23619281111033	17.23037903524881
8	21.286475327773847	43.84465771169814	21.182834210875125	13.686032749652885
9	26.118031486218317	15.551666859378042	22.330265932295323	36.00003572210832
10	14.324847452496648	30.352605410771343	36.72284438227544	18.59970275445657
11	31.964847565303305	21.542311427220433	30.15171555425201	16.34112545322425
12	24.77685432994255	25.010740133882702	34.871781132522436	15.340624403652303
13	29.10858298856798	24.174889801996112	27.44496680194065	19.27156040749525
14	17.83675372520486	26.930474436601248	28.594278634325	26.63849320386889
15	19.205145487686686	39.781549907075515	23.4042793205655	17.6090252846723
16	17.50735828428594	29.34937820030138	34.713193772696464	18.430069742716213
17	17.578943509245914	32.69470664158599	31.619236167318594	18.1071136818495
18	17.65927125018919	28.20659975351745	38.04352832904198	16.09060066725138
19	23.12245068703955	26.83378973026988	28.992956164272815	21.050803418417754
20	22.65341000425845	30.561720752758823	30.73854518893705	16.04632405404567
21	20.12809195998748	27.82944949410914	28.09755331759681	23.944905228306574
22	21.696151506861934	33.57130837862051	28.358277705550183	16.374262408967375
23	18.013437153060774	33.04111708673046	31.1615701558706	17.783875604338167
24	22.55733633399043	28.83629591818509	31.78609601538683	16.820271732437647
25	19.77467810150155	31.276256924683693	31.831594700719236	17.117470273095517
26	15.943858006499545	32.831484714227834	33.37023051100476	17.854426768267864
27	17.77339398571304	32.677080601297085	31.09562526380307	18.453900149186804
28	16.74713541593225	32.88859308476386	29.51022169328434	20.85404980601955
29	19.80711001563312	29.630501792215775	28.65975349865149	21.90263469349961
30	17.56512469365942	30.057333983851724	31.320392529567094	21.05714879292176
31	21.513686737791264	28.05008051575204	30.628511694760224	19.807721051696472
32	18.166384179994303	32.12761817202451	30.945874425508595	18.76012322247259
33	17.058246777724822	32.604414312532725	33.4438368552512	16.893502054491254
34	18.5458845781078	31.614065862167184	33.13455860164866	16.705490958076346
35	17.673419085194407	32.89799363958461	31.69580368633357	17.732783588887415
36	16.6557620230746	31.07555507926078	33.545832875056284	18.722850022608334
37	17.57950754253516	30.96133833818872	28.944919329138806	22.514234790137312
38	18.656905130540803	33.807544321265844	29.1238588901517	18.411691658041658
39	21.188098521574744	29.084517568226875	29.89038013023529	19.837003779963094
40	22.5884521704471	28.946752437328847	31.584877139448768	16.879918252775276
41	21.797724501700092	27.017711585337768	33.13385356003711	18.05071035292503
42	19.108695795225835	32.48700138282161	31.588590358602964	16.815712463349584
43	16.347329819405942	32.92600729295043	32.68643415334373	18.040228734299898
44	16.260844715055082	32.04710241998483	31.488051424795096	20.204001440164998
45	16.69190715636037	32.5801138783211	31.273248747141057	19.454730218177477
46	18.444234916325584	28.386835074631072	31.509044282295168	21.659885726748175
47	21.787524899719582	28.772090128759398	30.304427567315024	19.135957404205996
48	16.90929498659011	30.544517737436866	30.333240267840605	22.21294700813242
49	19.9430420383411	28.75183193312069	30.696336697791903	20.608789330746298
50	16.825731962794464	33.49997391344811	29.2632828726981	20.411011251059325
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	15.0
1	18.5
2	22.0
3	102.0
4	182.0
5	202.5
6	223.0
7	235.0
8	247.0
9	391.5
10	536.0
11	801.0
12	1066.0
13	2010.5
14	2955.0
15	4153.5
16	5352.0
17	7116.5
18	8881.0
19	10162.0
20	11443.0
21	13773.0
22	16103.0
23	18358.5
24	20614.0
25	27574.0
26	34534.0
27	49873.0
28	65212.0
29	83699.5
30	102187.0
31	122834.0
32	143481.0
33	178898.5
34	214316.0
35	250365.5
36	286415.0
37	282905.0
38	279395.0
39	249215.0
40	219035.0
41	193104.5
42	167174.0
43	143749.0
44	120324.0
45	110230.5
46	100137.0
47	91386.0
48	82635.0
49	72514.5
50	62394.0
51	57086.0
52	51778.0
53	44996.5
54	38215.0
55	32902.5
56	27590.0
57	24761.5
58	21933.0
59	17843.5
60	13754.0
61	11503.5
62	9253.0
63	7732.0
64	6211.0
65	5281.5
66	4352.0
67	3694.0
68	3036.0
69	2642.5
70	2249.0
71	1894.5
72	1540.0
73	1269.0
74	998.0
75	870.0
76	742.0
77	584.5
78	427.0
79	340.0
80	253.0
81	192.0
82	131.0
83	109.0
84	87.0
85	70.5
86	54.0
87	39.5
88	25.0
89	19.5
90	14.0
91	10.0
92	6.0
93	3.5
94	1.0
95	1.0
96	1.0
97	3.0
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.016215957065786022
2	0.005264310699617491
3	5.170305151410036E-4
4	1.880110964149104E-4
5	4.70027741037276E-5
6	7.050416115559141E-4
7	4.70027741037276E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	9.40055482074552E-5
47	0.0
48	0.0
49	0.0
50	4.70027741037276E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2127534.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.29216240425682
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.11061867438875	46.470027378028256
2	12.294046838722338	14.087050561792406
3	3.172118630083609	5.452126071609563
4	1.1437191803996953	2.621045801132914
5	0.5671187048215491	1.6245728469563985
6	0.3424058284448229	1.1770302198854936
7	0.21864353522627863	0.876859265017736
8	0.1624336630760233	0.7444940643895889
9	0.11568535366483439	0.5965077662963619
>10	0.7200847707178925	7.758376515430039
>50	0.0720801092655934	2.904438947088241
>100	0.06932829687198186	8.069724161323844
>500	0.007838446197882798	3.152788717315586
>1k	0.0035479282790416814	3.2421795781663763
>5k	3.3003983991085404E-4	1.2227781055671663
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	7926	0.37254398754614493	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	6358	0.2988436377515001	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	5804	0.272804100898035	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	5779	0.27162903154544177	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	4073	0.19144229892448253	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	3272	0.1537930768673967	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA	2558	0.12023309615733521	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA	2420	0.1137467133310208	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT	2311	0.10862341095371449	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	2174	0.10218403090150381	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	4.70027741037276E-5	0.0
8	0.0	0.0	0.0	4.70027741037276E-5	0.0
9	0.0	0.0	0.0	4.70027741037276E-5	0.0
10	0.0	0.0	0.0	4.70027741037276E-5	0.0
11	0.0	0.0	0.0	4.70027741037276E-5	0.0
12	0.0	0.0	0.0	4.70027741037276E-5	0.0
13	0.0	0.0	0.0	4.70027741037276E-5	0.0
14	0.0	0.0	0.0	4.70027741037276E-5	0.0
15	0.0	0.0	0.0	4.70027741037276E-5	0.0
16	0.0	0.0	0.0	4.70027741037276E-5	0.0
17	0.0	0.0	0.0	4.70027741037276E-5	0.0
18	0.0	0.0	0.0	4.70027741037276E-5	0.0
19	0.0	0.0	0.0	9.40055482074552E-5	0.0
20	4.70027741037276E-5	0.0	0.0	2.8201664462236564E-4	0.0
21	4.70027741037276E-5	0.0	0.0	5.640332892447313E-4	0.0
22	4.70027741037276E-5	0.0	0.0	9.40055482074552E-4	0.0
23	4.70027741037276E-5	0.0	0.0	0.0019271137382528317	0.0
24	9.40055482074552E-5	0.0	0.0	0.003055180316742294	0.0
25	9.40055482074552E-5	0.0	0.0	0.004183246895231757	0.0
26	9.40055482074552E-5	0.0	0.0	0.005593330118343585	0.0
27	9.40055482074552E-5	0.0	0.0	0.006956410567351685	0.0
28	9.40055482074552E-5	4.70027741037276E-5	0.0	0.013066771200836273	0.0
29	9.40055482074552E-5	4.70027741037276E-5	0.0	0.022608334343892977	0.0
30	9.40055482074552E-5	4.70027741037276E-5	0.0	0.04653274636269033	0.0
31	9.40055482074552E-5	4.70027741037276E-5	0.0	0.11463976603899162	0.0
32	9.40055482074552E-5	4.70027741037276E-5	0.0	0.20465007844762997	0.0
33	9.40055482074552E-5	4.70027741037276E-5	0.0	0.2937203353741938	0.0
34	9.40055482074552E-5	4.70027741037276E-5	0.0	0.3983015077549877	0.0
35	9.40055482074552E-5	4.70027741037276E-5	0.0	0.5096980823808221	0.0
36	9.40055482074552E-5	4.70027741037276E-5	0.0	0.6684264505291102	0.0
37	9.40055482074552E-5	4.70027741037276E-5	0.0	0.9249205888131518	0.0
38	9.40055482074552E-5	4.70027741037276E-5	0.0	1.249615752321702	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTGTCG	25	0.0023532552	35.199814	8
GCGACGA	55	4.9635837E-6	27.99985	20
TAGGACC	3025	0.0	24.727139	4
TGTCCGC	90	1.5141268E-8	24.444315	10
GTCCGCT	85	2.3545181E-7	23.293993	11
GGCGACG	60	2.873875E-4	21.999884	19
TGTAGGA	9555	0.0	21.252087	2
CTGTAGG	9270	0.0	21.173729	1
GGACGTC	75	5.475366E-5	20.533224	6
AGGACCT	6175	0.0	20.414467	5
GTAGGAC	10060	0.0	20.09731	3
GTCCTAT	430	0.0	19.957603	1
GGACCTG	6145	0.0	19.941309	6
TAGGACA	2240	0.0	19.544538	4
TTAGGAC	5720	0.0	19.422974	3
TAGGACG	10395	0.0	19.343813	4
GCGACAA	80	8.993035E-5	19.249897	20
CGACGAA	80	8.993035E-5	19.249897	21
GACGTGT	115	2.0233165E-7	19.130333	7
AGGACGT	10330	0.0	19.124777	5
>>END_MODULE