FastQCFastQC Report
Thu 2 Feb 2017
SRR4062466_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062466_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1480138
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30130.20356210029064858No Hit
GTACATGGAAGCAGTGGTATCAACG29100.19660328969325833No Hit
TCCATGTACTCTGCGTTGATACCAC25850.17464587761411435No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21570.14572965493758014No Hit
TATCAACGCAGAGTACTTTTTTTTT20830.1407301211103289No Hit
GAGTACTTTTTTTTTTTTTTTTTTT16070.10857095757287497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCTA759.640753E-713.937304516
CGTCGTA1850.013.86553110
CCGTCGT2050.012.9762349
CGAACGA1207.366907E-1012.67027816
CGTTATT905.432321E-712.6612782
GTCGTAG2050.012.51237311
CAAGACG2550.012.2926284
TAAGCCC701.0920646E-412.2128074
TCGAACT550.003061781412.09435519
ACGGTAT2200.012.0914919
CGCATCG2050.012.04854413
CGTCTAT951.0471394E-611.9928671
AGCCCCG2500.011.78335816
CGCGTAT658.0175017E-411.6932657
GCGCGCT907.458575E-611.61127715
GCATCGC2300.011.56499214
ACGCCCC1406.7666406E-1011.53900117
GCGTTAT1001.943028E-611.3932231
CGCAAGA2600.011.32210352
ACCGTCG2350.011.3200768
Produced by FastQC (version 0.11.5)