##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062466_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1480138
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.143546750370575	32.0	32.0	32.0	32.0	32.0
2	30.771072697275525	32.0	32.0	32.0	32.0	32.0
3	30.76765274589261	32.0	32.0	32.0	32.0	32.0
4	30.84800471307405	32.0	32.0	32.0	32.0	32.0
5	30.62402019271176	32.0	32.0	32.0	32.0	32.0
6	34.282265572534456	36.0	36.0	36.0	32.0	36.0
7	34.15190543043959	36.0	36.0	36.0	32.0	36.0
8	34.14070039415244	36.0	36.0	36.0	32.0	36.0
9	34.31740959288931	36.0	36.0	36.0	32.0	36.0
10	33.954419114974414	36.0	36.0	36.0	32.0	36.0
11	34.31393964616813	36.0	36.0	36.0	32.0	36.0
12	34.067836917909005	36.0	36.0	36.0	32.0	36.0
13	34.19867404255549	36.0	36.0	36.0	32.0	36.0
14	34.10179118433552	36.0	36.0	36.0	32.0	36.0
15	34.037120187441985	36.0	36.0	36.0	32.0	36.0
16	34.03783768810746	36.0	36.0	36.0	32.0	36.0
17	33.968050952005825	36.0	36.0	36.0	32.0	36.0
18	33.97699673949321	36.0	36.0	36.0	32.0	36.0
19	33.972303258209706	36.0	36.0	36.0	32.0	36.0
20	33.951920023673466	36.0	36.0	36.0	32.0	36.0
21	33.92405910800209	36.0	36.0	36.0	32.0	36.0
22	33.879078842648454	36.0	36.0	36.0	32.0	36.0
23	33.835528849337024	36.0	36.0	36.0	32.0	36.0
24	33.808765804269605	36.0	36.0	36.0	32.0	36.0
25	33.337660407340394	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	6.0
4	57.0
5	138.0
6	252.0
7	54.0
8	224.0
9	291.0
10	169.0
11	59.0
12	118.0
13	91.0
14	290.0
15	325.0
16	486.0
17	710.0
18	963.0
19	1268.0
20	2074.0
21	2939.0
22	4520.0
23	6791.0
24	9614.0
25	13420.0
26	18561.0
27	23857.0
28	32129.0
29	42311.0
30	54881.0
31	75399.0
32	106528.0
33	153868.0
34	331876.0
35	595867.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.50899174607979	17.6599838921531	11.007186369950757	25.82383799181635
2	16.438753214322873	20.392150768802523	37.65983782111137	25.50925819576324
3	19.01339934813982	24.754066018409915	28.273759654935702	27.958774978514562
4	12.20750068584881	15.636069025446004	36.88082718096543	35.275603107739755
5	14.039693026179284	37.18546503965585	34.140327992944066	14.634513941220797
6	33.25681856740295	36.09171768214333	17.476999945250036	13.174463805203679
7	29.553502975429886	30.857729694938996	21.438645026684895	18.150122302946222
8	27.722913547587847	33.18240034839012	20.122639812496875	18.972046291525167
9	27.366046279809105	14.460056495532228	18.905241830498525	39.26865539416015
10	15.296752534746439	27.172535923422892	32.55474166183584	24.97596987999483
11	36.94733694909568	21.520595369677654	22.620543825447697	18.911523855778974
12	24.291933470667626	24.2485006545366	29.473292538959306	21.986273335836472
13	29.319727661071294	20.081241692343596	25.579633431531384	25.01939721505373
14	22.992536551146575	20.346066451956375	25.880369702172334	30.781027294724716
15	24.785296904771727	27.87883420125443	22.973248099175006	24.362620794798833
16	25.085968063980264	26.30169582321016	24.70144529157445	23.910890821235125
17	23.331132619183553	26.320969649085306	26.079747928036646	24.268149803694495
18	24.043916583117188	25.294926280268925	27.3264978358317	23.334659300782185
19	24.785018580645684	25.874555441223528	25.88010291235469	23.460323065776095
20	24.94070884219555	25.072076130793658	26.091356411224258	23.895858615786533
21	26.044656719649023	24.948297382227288	24.785325048286218	24.221720849837467
22	24.792592116436666	25.411449470019658	25.62851642719156	24.167441986352117
23	23.649208648474566	25.17501205988196	26.040481962273038	25.13529732937043
24	23.91542753381498	25.89869408172695	25.962085545325575	24.22379283913249
25	24.192618730947775	25.40654889072599	25.888697812300855	24.512134566025377
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	242.0
1	242.0
2	589.5
3	937.0
4	937.0
5	937.0
6	2434.5
7	3932.0
8	3932.0
9	3932.0
10	4173.0
11	4414.0
12	4414.0
13	4414.0
14	4817.5
15	5221.0
16	5221.0
17	5221.0
18	8691.5
19	12162.0
20	12162.0
21	12162.0
22	19358.0
23	26554.0
24	26554.0
25	26554.0
26	39171.5
27	51789.0
28	51789.0
29	51789.0
30	64072.5
31	76356.0
32	76356.0
33	76356.0
34	91127.5
35	105899.0
36	105899.0
37	105899.0
38	116996.5
39	128094.0
40	128094.0
41	128094.0
42	143466.5
43	158839.0
44	158839.0
45	158839.0
46	179291.0
47	199743.0
48	199743.0
49	199743.0
50	195362.0
51	190981.0
52	190981.0
53	190981.0
54	173602.0
55	156223.0
56	156223.0
57	156223.0
58	141521.5
59	126820.0
60	126820.0
61	126820.0
62	111790.0
63	96760.0
64	96760.0
65	96760.0
66	80109.5
67	63459.0
68	63459.0
69	63459.0
70	48032.5
71	32606.0
72	32606.0
73	32606.0
74	25621.0
75	18636.0
76	18636.0
77	18636.0
78	15233.5
79	11831.0
80	11831.0
81	11831.0
82	8284.0
83	4737.0
84	4737.0
85	4737.0
86	3501.5
87	2266.0
88	2266.0
89	2266.0
90	1525.0
91	784.0
92	784.0
93	784.0
94	480.5
95	177.0
96	177.0
97	177.0
98	426.5
99	676.0
100	676.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0077019845446843465
2	0.0029726957891764143
3	0.004459043683764622
4	0.014863478945882075
5	0.036010155809796114
6	0.04627946853604191
7	0.06918273836628747
8	0.09026185396226567
9	0.09708554202378426
10	0.12059686326545227
11	0.12134003721274637
12	0.1346496069961044
13	0.12383980412637201
14	0.12613688723619013
15	0.11492171675884276
16	0.11363805266806204
17	0.1236371203225645
18	0.13323082036945205
19	0.13424423938848945
20	0.1243127330019228
21	0.13329838163738786
22	0.15221553665941961
23	0.1416759788614305
24	0.1365413224983076
25	0.1326903302259654
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1480138.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.63537821224105
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.07875822402809	42.1122710769966
2	14.364472210223598	15.696167440495849
3	4.376238671246875	7.172923648518245
4	1.6452234989468735	3.59549632434516
5	0.8016005853508025	2.1897875577897454
6	0.4212113169419913	1.3807823765041096
7	0.24648930872581998	0.9426925625256316
8	0.16840400036875156	0.7360653002080898
9	0.11148326039004987	0.5481837086169713
>10	0.6048582105015841	6.259363793671363
>50	0.0780114627795643	2.994300779282667
>100	0.09083956289185119	10.408003873991918
>500	0.009183168827035706	3.3406130972975694
>1k	0.0032265187770665997	2.6233484597559933
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3013	0.20356210029064858	No Hit
GTACATGGAAGCAGTGGTATCAACG	2910	0.19660328969325833	No Hit
TCCATGTACTCTGCGTTGATACCAC	2585	0.17464587761411435	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2157	0.14572965493758014	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2083	0.1407301211103289	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1607	0.10857095757287497	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	6.75612679358276E-5	0.0
10	0.0	0.0	0.0	6.75612679358276E-5	0.0
11	0.0	0.0	0.0	6.75612679358276E-5	0.0
12	0.0	0.0	0.0	1.351225358716552E-4	0.0
13	0.0	0.0	0.0	1.351225358716552E-4	6.75612679358276E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGCTA	75	9.640753E-7	13.9373045	16
CGTCGTA	185	0.0	13.865531	10
CCGTCGT	205	0.0	12.976234	9
CGAACGA	120	7.366907E-10	12.670278	16
CGTTATT	90	5.432321E-7	12.661278	2
GTCGTAG	205	0.0	12.512373	11
CAAGACG	255	0.0	12.292628	4
TAAGCCC	70	1.0920646E-4	12.212807	4
TCGAACT	55	0.0030617814	12.094355	19
ACGGTAT	220	0.0	12.091491	9
CGCATCG	205	0.0	12.048544	13
CGTCTAT	95	1.0471394E-6	11.992867	1
AGCCCCG	250	0.0	11.783358	16
CGCGTAT	65	8.0175017E-4	11.693265	7
GCGCGCT	90	7.458575E-6	11.611277	15
GCATCGC	230	0.0	11.564992	14
ACGCCCC	140	6.7666406E-10	11.539001	17
GCGTTAT	100	1.943028E-6	11.393223	1
CGCAAGA	260	0.0	11.3221035	2
ACCGTCG	235	0.0	11.320076	8
>>END_MODULE