FastQCFastQC Report
Thu 2 Feb 2017
SRR4062466_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062466_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1480138
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATA407.033041E-427.49989544
GAACCGT450.001398393224.444356
TAGACCG654.9330114E-420.3076135
TTAACGG2300.018.17384335
ATACCGT4250.018.1175776
CCGTCGT4150.018.0240299
CGTCGTA4200.017.80945610
TTAGGAC4500.017.5999343
ACCGTCG4400.017.4999338
TACCGTC4350.017.1953377
CGCAATA4500.017.11104636
ACAGCGT1307.3198134E-716.9230128
ACCGTCC1307.3198134E-716.9230128
GTCCTAT4300.016.8853661
CGAATGC4700.016.85143
TAGTACG1053.773204E-516.7629722
GTCCTAA3700.016.6502721
TATGGCG6350.016.62985816
TAGCGGC4500.016.62215830
TGGCGAG6250.016.54393618