FastQCFastQC Report
Thu 2 Feb 2017
SRR4062465_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062465_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences808553
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG81921.0131679679625205No Hit
TCCATGTACTCTGCGTTGATACCAC60190.7444162596638687No Hit
GAGTACATGGAAGCAGTGGTATCAA35390.4376954881127149No Hit
CATGTACTCTGCGTTGATACCACTG28430.3516157877096492No Hit
GCTTCCATGTACTCTGCGTTGATAC20630.2551471579475928No Hit
CATGGAAGCAGTGGTATCAACGCAG18800.2325141332726488No Hit
GCGTTGATACCACTGCTTCCATGTA17680.21866222746066122No Hit
GTACATGGGAAGCAGTGGTATCAAC17330.21433350689441508No Hit
CCCATGTACTCTGCGTTGATACCAC17190.21260201866791661No Hit
GTATCAACGCAGAGTACATGGAAGC14400.17809593186841186No Hit
GTACTTTTTTTTTTTTTTTTTTTTT12820.15855485045507223No Hit
ACGCAGAGTACATGGAAGCAGTGGT12260.15162889754907843No Hit
CAGTGGTATCAACGCAGAGTACATG11910.1473001769828323No Hit
ACTCTGCGTTGATACCACTGCTTCC11420.14123996819008772No Hit
GTATCAACGCAGAGTACTTTTTTTT10180.12590392961253005No Hit
TATCAACGCAGAGTACATGGAAGCA8960.11081524649590072No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCTA402.7607026E-416.62375
TATTCCC807.70342E-915.4362935
GCGTTAT807.792551E-915.4229191
CGTTATT704.4665467E-714.9172412
TTATAAC456.7963684E-414.7665612
CGTCGTA1006.566552E-1014.25241610
TAATACC400.00528110514.2480034
GTTATTC801.2919008E-714.2444733
ATTCCCA954.8803486E-914.0015076
ACTCGCC551.9555006E-413.8205258
CCGTCGT903.6221536E-813.7245499
AGAACCG903.633795E-813.72114855
CGGTTCT1257.2759576E-1213.6823212
CGCATCG655.44699E-513.15363313
GTATAAT655.4946642E-513.1414221
GTCCTAG802.0173047E-613.0501621
GTCGTAG1102.730303E-912.95674211
ACTGTTC1550.012.873158
AGGGACG1207.348717E-1012.6688158
GGTTCTA1301.9645086E-1012.42287513
Produced by FastQC (version 0.11.5)