##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062465_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	808553
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.691616999751407	32.0	21.0	32.0	14.0	32.0
2	24.6036338990765	32.0	14.0	32.0	14.0	32.0
3	24.569986135726413	32.0	14.0	32.0	14.0	32.0
4	24.551213093019257	32.0	14.0	32.0	14.0	32.0
5	23.282112613520695	27.0	14.0	32.0	14.0	32.0
6	27.15978049676397	32.0	14.0	36.0	14.0	36.0
7	26.774795220597785	32.0	14.0	36.0	14.0	36.0
8	26.429822163791364	32.0	14.0	36.0	14.0	36.0
9	27.541775245407536	32.0	14.0	36.0	14.0	36.0
10	24.971561542657067	27.0	14.0	36.0	14.0	36.0
11	28.065230108601416	36.0	14.0	36.0	14.0	36.0
12	26.17804769755353	32.0	14.0	36.0	14.0	36.0
13	27.215642017282725	32.0	14.0	36.0	14.0	36.0
14	26.356865907367855	32.0	14.0	36.0	14.0	36.0
15	26.151606635557595	32.0	14.0	36.0	14.0	36.0
16	26.219995473395066	32.0	14.0	36.0	14.0	36.0
17	25.9240631102723	32.0	14.0	36.0	14.0	36.0
18	26.180601642687616	32.0	14.0	36.0	14.0	36.0
19	25.953849654877292	32.0	14.0	36.0	14.0	36.0
20	26.044404015568553	32.0	14.0	36.0	14.0	36.0
21	26.033075135458034	32.0	14.0	36.0	14.0	36.0
22	25.812655447447476	32.0	14.0	36.0	14.0	36.0
23	25.6988088597779	32.0	14.0	36.0	14.0	36.0
24	25.68604037088478	32.0	14.0	36.0	14.0	36.0
25	25.386088481521927	32.0	14.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	warn
#Quality	Count
2	1.0
3	19.0
4	61.0
5	101.0
6	148.0
7	111.0
8	96.0
9	90.0
10	67.0
11	40.0
12	50.0
13	42.0
14	470.0
15	1354.0
16	3599.0
17	9095.0
18	17988.0
19	26371.0
20	39209.0
21	49735.0
22	59075.0
23	67985.0
24	69857.0
25	69128.0
26	67333.0
27	60391.0
28	55620.0
29	49819.0
30	43086.0
31	37446.0
32	31044.0
33	22776.0
34	18309.0
35	8037.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.544439854246086	16.77963502150934	10.89468463275802	23.781240491486557
2	18.305330010451	19.49179689190944	36.46249080126401	25.740382296375543
3	22.069969475241304	24.106643711256563	27.614517317976954	26.208869495525178
4	13.460919989510714	13.877646441806728	36.39027564061333	36.27115792806923
5	15.84782877644439	36.42744030681678	33.36941729555858	14.355313621180255
6	36.220922535568505	33.85092591332407	17.384800594880907	12.543350956226515
7	31.90876386195528	29.822772403431934	21.101110441466382	17.1673532931464
8	31.343784232056986	31.2082484945631	20.052728971846935	17.395238301532977
9	29.45143500838661	14.125656848242524	18.593277092475567	37.829631050895294
10	17.918970653420136	25.83197343430016	31.929851496549283	24.319204415730418
11	39.11694968767605	20.704632547312897	21.41223666045527	18.76618110455578
12	26.251129960993126	23.222958330753514	29.013931025942668	21.511980682310693
13	32.63117361335527	19.134378586790582	24.604315477848722	23.63013232200542
14	26.086622743206245	19.041327758083348	24.96146069238265	29.910588806327752
15	28.158997629026167	25.990330388703516	23.560051257606617	22.290620724663697
16	28.262048438896713	24.50309397819482	24.871177098370925	22.363680484537536
17	26.06633251942726	24.727473539798027	26.447696216574958	22.758497724199756
18	27.50371174794538	22.816198204244582	27.91630704916348	21.76378299864656
19	27.119074331369358	26.00198626455954	24.881183239097215	21.997756164973882
20	26.847394332015888	22.959322655096926	27.37684863253197	22.816434380355215
21	28.26360726381158	24.275940636357447	23.559837288639304	23.90061481119167
22	26.702172459065128	25.0236567663306	24.657905818821373	23.616264955782903
23	25.668121383847193	24.151814020765634	25.09139454703971	25.088670048347467
24	25.979624261172045	25.76217077033766	25.023002269887524	23.235202698602777
25	29.197496083761077	22.387017280984725	24.170840892345225	24.244645742908975
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	128.0
1	128.0
2	332.5
3	537.0
4	537.0
5	537.0
6	1457.5
7	2378.0
8	2378.0
9	2378.0
10	2554.0
11	2730.0
12	2730.0
13	2730.0
14	2887.0
15	3044.0
16	3044.0
17	3044.0
18	4412.5
19	5781.0
20	5781.0
21	5781.0
22	9080.0
23	12379.0
24	12379.0
25	12379.0
26	18225.0
27	24071.0
28	24071.0
29	24071.0
30	30287.0
31	36503.0
32	36503.0
33	36503.0
34	42712.0
35	48921.0
36	48921.0
37	48921.0
38	53870.0
39	58819.0
40	58819.0
41	58819.0
42	68295.0
43	77771.0
44	77771.0
45	77771.0
46	100699.5
47	123628.0
48	123628.0
49	123628.0
50	113012.0
51	102396.0
52	102396.0
53	102396.0
54	91726.0
55	81056.0
56	81056.0
57	81056.0
58	73922.5
59	66789.0
60	66789.0
61	66789.0
62	60617.0
63	54445.0
64	54445.0
65	54445.0
66	47553.5
67	40662.0
68	40662.0
69	40662.0
70	33181.0
71	25700.0
72	25700.0
73	25700.0
74	20917.5
75	16135.0
76	16135.0
77	16135.0
78	13440.0
79	10745.0
80	10745.0
81	10745.0
82	8406.0
83	6067.0
84	6067.0
85	6067.0
86	4866.5
87	3666.0
88	3666.0
89	3666.0
90	2842.5
91	2019.0
92	2019.0
93	2019.0
94	1558.5
95	1098.0
96	1098.0
97	1098.0
98	1091.5
99	1085.0
100	1085.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008286407941099716
2	0.002226199148355148
3	0.0035866541834610717
4	0.013480872620595065
5	0.031290465807436246
6	0.040071584670392665
7	0.06134415431023074
8	0.08014316934078533
9	0.08880061047327757
10	0.11069156876543652
11	0.11106260195682906
12	0.12404876365556743
13	0.11155731287868575
14	0.11576235571446769
15	0.1073522700429038
16	0.10574445954686952
17	0.11440190067936178
18	0.12145153131581975
19	0.12454347457742411
20	0.11489661160121846
21	0.1221935976986048
22	0.14507397783447715
23	0.13171678294434627
24	0.12664599599531506
25	0.12590392961253005
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	808553.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.39185690579966
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.33338615844119	58.41676004583331
2	6.929317070297703	9.062418206316446
3	1.6843874534119927	3.3043566998232397
4	0.6038174520915496	1.579389776975806
5	0.328315598529475	1.07345833194907
6	0.19222108144586272	0.754181607131155
7	0.12010953904231518	0.5497930053053643
8	0.09731699391044744	0.5090991152235644
9	0.06842346053243517	0.40269034261229675
>10	0.49647471515730024	6.591221767150235
>50	0.07753202611315152	3.6184038706218122
>100	0.062417449792755195	7.98914060457478
>500	0.0033367819054539428	1.393004031836429
>1k	0.0025516567512294855	2.9320726161680075
>5k	3.925625771122286E-4	1.8240099784785306
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	8192	1.0131679679625205	No Hit
TCCATGTACTCTGCGTTGATACCAC	6019	0.7444162596638687	No Hit
GAGTACATGGAAGCAGTGGTATCAA	3539	0.4376954881127149	No Hit
CATGTACTCTGCGTTGATACCACTG	2843	0.3516157877096492	No Hit
GCTTCCATGTACTCTGCGTTGATAC	2063	0.2551471579475928	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1880	0.2325141332726488	No Hit
GCGTTGATACCACTGCTTCCATGTA	1768	0.21866222746066122	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1733	0.21433350689441508	No Hit
CCCATGTACTCTGCGTTGATACCAC	1719	0.21260201866791661	No Hit
GTATCAACGCAGAGTACATGGAAGC	1440	0.17809593186841186	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1282	0.15855485045507223	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	1226	0.15162889754907843	No Hit
CAGTGGTATCAACGCAGAGTACATG	1191	0.1473001769828323	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1142	0.14123996819008772	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1018	0.12590392961253005	No Hit
TATCAACGCAGAGTACATGGAAGCA	896	0.11081524649590072	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.236777304641749E-4	0.0
3	0.0	0.0	0.0	1.236777304641749E-4	0.0
4	0.0	0.0	0.0	1.236777304641749E-4	0.0
5	0.0	0.0	0.0	1.236777304641749E-4	0.0
6	0.0	0.0	0.0	1.236777304641749E-4	0.0
7	0.0	0.0	0.0	1.236777304641749E-4	0.0
8	0.0	0.0	0.0	1.236777304641749E-4	0.0
9	0.0	0.0	0.0	1.236777304641749E-4	0.0
10	0.0	0.0	0.0	1.236777304641749E-4	0.0
11	0.0	0.0	0.0	2.473554609283498E-4	0.0
12	0.0	0.0	0.0	2.473554609283498E-4	0.0
13	0.0	0.0	0.0	2.473554609283498E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACCTA	40	2.7607026E-4	16.6237	5
TATTCCC	80	7.70342E-9	15.436293	5
GCGTTAT	80	7.792551E-9	15.422919	1
CGTTATT	70	4.4665467E-7	14.917241	2
TTATAAC	45	6.7963684E-4	14.766561	2
CGTCGTA	100	6.566552E-10	14.252416	10
TAATACC	40	0.005281105	14.248003	4
GTTATTC	80	1.2919008E-7	14.244473	3
ATTCCCA	95	4.8803486E-9	14.001507	6
ACTCGCC	55	1.9555006E-4	13.820525	8
CCGTCGT	90	3.6221536E-8	13.724549	9
AGAACCG	90	3.633795E-8	13.7211485	5
CGGTTCT	125	7.2759576E-12	13.68232	12
CGCATCG	65	5.44699E-5	13.153633	13
GTATAAT	65	5.4946642E-5	13.141422	1
GTCCTAG	80	2.0173047E-6	13.050162	1
GTCGTAG	110	2.730303E-9	12.956742	11
ACTGTTC	155	0.0	12.87315	8
AGGGACG	120	7.348717E-10	12.668815	8
GGTTCTA	130	1.9645086E-10	12.422875	13
>>END_MODULE