FastQCFastQC Report
Thu 2 Feb 2017
SRR4062465_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062465_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences808553
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA51520.6371876673514291No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC48220.5963740162982514No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA23340.28866382290338416No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC19260.23820330887400085No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG13380.16548080336106602No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA13200.16325460421271085No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG12060.1491553429397949No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC11990.1482895988265457No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10250.12676967372577927No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA9900.12244095315953314No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC9600.11873062124560789No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA9390.11613338890586022No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT8930.11044421330450818No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT8710.10772330323429632No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA8440.1043840045117636No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA8090.10005528394551749No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGAC500.002579831821.9998153
CTATACT1057.7388904E-820.9522044
GTATAGC1502.1827873E-1120.538241
CGCCCCT1900.019.6840445
CGGTCCA2950.019.38966810
AATAGAG808.984462E-519.2498385
CCTATAT708.107109E-418.861651
GTATAGG1051.7870989E-618.8616491
GTGTATA3700.018.4368381
GATATAC2750.018.4043961
AACGTGG600.007410392618.333185
CATTCGA1801.4551915E-1118.3331841
TGGTATA2650.018.26399644
TAACGGC1456.2991603E-918.20674336
ACTATAC851.4301829E-418.1174953
CGCAATA2700.017.92577636
TTAGGAC2600.017.7690813
TATATCA2650.017.4338154
TAGCGGC2800.017.28556830
CTAGCGG2800.017.28556829
Produced by FastQC (version 0.11.5)