##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062465_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	808553
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.240034976062176	32.0	32.0	32.0	32.0	32.0
2	31.414022333724567	32.0	32.0	32.0	32.0	32.0
3	31.4959365681656	32.0	32.0	32.0	32.0	32.0
4	31.608869177407048	32.0	32.0	32.0	32.0	32.0
5	31.54493397464359	32.0	32.0	32.0	32.0	32.0
6	35.13637695982823	36.0	36.0	36.0	36.0	36.0
7	35.15638925339464	36.0	36.0	36.0	36.0	36.0
8	35.095727800156574	36.0	36.0	36.0	36.0	36.0
9	35.21092989575204	36.0	36.0	36.0	36.0	36.0
10	35.10353557528078	36.0	36.0	36.0	36.0	36.0
11	35.22178261660027	36.0	36.0	36.0	36.0	36.0
12	35.1320247404932	36.0	36.0	36.0	36.0	36.0
13	35.17512395600536	36.0	36.0	36.0	36.0	36.0
14	35.13688774885505	36.0	36.0	36.0	36.0	36.0
15	35.11178859023465	36.0	36.0	36.0	36.0	36.0
16	35.10682911324304	36.0	36.0	36.0	36.0	36.0
17	35.08414909103052	36.0	36.0	36.0	36.0	36.0
18	35.09435745090303	36.0	36.0	36.0	36.0	36.0
19	35.06183886523209	36.0	36.0	36.0	36.0	36.0
20	35.04959971702535	36.0	36.0	36.0	36.0	36.0
21	35.02985951446597	36.0	36.0	36.0	36.0	36.0
22	34.99687219019656	36.0	36.0	36.0	36.0	36.0
23	34.92787609470251	36.0	36.0	36.0	32.0	36.0
24	34.91935964618275	36.0	36.0	36.0	32.0	36.0
25	34.884613624586144	36.0	36.0	36.0	32.0	36.0
26	34.83224723673031	36.0	36.0	36.0	32.0	36.0
27	34.806712732498674	36.0	36.0	36.0	32.0	36.0
28	34.76709628187639	36.0	36.0	36.0	32.0	36.0
29	34.73699930616793	36.0	36.0	36.0	32.0	36.0
30	34.70572862879737	36.0	36.0	36.0	32.0	36.0
31	34.69231454215122	36.0	36.0	36.0	32.0	36.0
32	34.647324294140276	36.0	36.0	36.0	32.0	36.0
33	34.61154432671699	36.0	36.0	36.0	32.0	36.0
34	34.583636446837744	36.0	36.0	36.0	32.0	36.0
35	34.50042359622684	36.0	36.0	36.0	32.0	36.0
36	34.47266536640146	36.0	36.0	36.0	32.0	36.0
37	34.415128012634916	36.0	36.0	36.0	32.0	36.0
38	34.32753449681097	36.0	36.0	36.0	32.0	36.0
39	34.22308617987937	36.0	36.0	36.0	32.0	36.0
40	34.13669728515014	36.0	36.0	36.0	32.0	36.0
41	34.132629524595174	36.0	36.0	36.0	32.0	36.0
42	33.99830685186995	36.0	36.0	36.0	32.0	36.0
43	34.023117841378365	36.0	36.0	36.0	32.0	36.0
44	33.98716472513243	36.0	36.0	36.0	32.0	36.0
45	33.82766002970739	36.0	36.0	36.0	32.0	36.0
46	33.93466105499578	36.0	36.0	36.0	32.0	36.0
47	33.837000171912045	36.0	36.0	36.0	32.0	36.0
48	33.8339243067554	36.0	36.0	36.0	32.0	36.0
49	33.86843286710952	36.0	36.0	36.0	32.0	36.0
50	33.2506724976594	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	3.0
21	18.0
22	86.0
23	242.0
24	595.0
25	1528.0
26	3157.0
27	6180.0
28	10393.0
29	16035.0
30	22895.0
31	32379.0
32	48304.0
33	69057.0
34	137819.0
35	459861.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.62535317199167	18.109868033667002	11.825509532551202	25.43926926179013
2	14.830623247576135	21.555602005137764	37.302834922445	26.3109398248411
3	18.584054378960804	25.735021297436887	28.895996279750864	26.784928043851448
4	11.57813369612269	16.626306350875023	36.87081813122256	34.92474182177973
5	12.9066369180499	38.0729525460916	34.5702755416157	14.450134994242802
6	33.22948488034135	35.87879537443572	17.640591181745098	13.251128563477831
7	28.48969515875293	31.35419861678655	22.09505387408602	18.061052350374496
8	27.777770906792753	33.37888796405431	20.731974279979173	18.11136684917377
9	26.946285524882104	14.665457922980929	18.986263114477346	39.40199343765962
10	14.945464304751821	27.621689610946966	33.27104098308954	24.16180510121167
11	36.51918921827017	21.600315625568143	22.281037854042964	19.599457302118722
12	23.149997588284254	24.936027693917406	30.112435424764982	21.801539293033358
13	29.99679674678098	20.06831957830841	25.701098134568788	24.23378554034182
14	23.169043958775738	20.447144466720175	25.755145302781635	30.628666271722448
15	25.08753291373602	27.71939501801366	24.250482033954484	22.942590034295833
16	25.23099908107446	25.970962942441624	25.81772623439651	22.98031174208741
17	22.90622878153937	26.542354057186106	27.482552164174766	23.06886499709976
18	24.651816269310732	24.252831910833304	29.049301653694936	22.04605016616103
19	24.052473987481342	27.678952400151875	25.94449590812229	22.324077704244498
20	23.839377257891567	24.551266274443357	28.408279976699113	23.20107649096596
21	25.134530451312404	26.043314414763163	24.535806558135338	24.286348575789095
22	23.461665469053976	26.566223859165696	25.83788570446217	24.13422496731816
23	22.403973518124353	25.68675151783495	26.177875785508185	25.731399178532516
24	22.69807916116816	27.551935370965168	26.171320865793586	23.578664602073086
25	24.44712962539252	25.165573561658917	25.475757309663067	24.9115395032855
26	22.348689572606865	26.06817363858646	27.12351571263727	24.459621076169405
27	24.77289676743516	26.428817900619993	26.145348542396107	22.652936789548736
28	23.274170029670287	24.88371201393106	26.847095985049833	24.995021971348816
29	22.70698395776158	26.342367167025536	27.606848283291264	23.343800591921617
30	24.293398206425554	25.484538428526022	27.413045279653904	22.809018085394523
31	23.05822871227984	25.80733730503752	26.100329848507148	25.034104134175493
32	22.67556981422368	26.5776022103684	26.315281744053888	24.431546231354037
33	22.427966997834403	25.87845200005442	26.8952066222004	24.79837437991078
34	22.647618647138778	26.698187997570972	27.91369273257288	22.74050062271737
35	24.927370252784915	25.914936930541348	26.618910572343434	22.538782244330307
36	23.09236376588795	26.787359641235643	26.61643701773415	23.50383957514226
37	24.752366264178104	26.30377971512072	26.26630536279007	22.67754865791111
38	23.206394633375922	26.445143361041268	25.80263755127988	24.545824454302934
39	23.400939703396066	25.985556914636394	26.50896106996078	24.104542312006757
40	24.87220998499789	26.09971145985483	25.68873036152237	23.339348193624907
41	22.48263255469957	26.36141353751702	27.325481446485266	23.830472461298147
42	25.586448878428502	26.856000781643257	26.018331513209397	21.53921882671884
43	23.927930512903917	25.059952779842508	25.97393120797276	25.038185499280814
44	23.36878349347538	25.933117556919584	27.342425295558858	23.35567365404618
45	24.251224100337268	26.133599158002013	26.366855357657442	23.248321384003276
46	23.098262321439613	25.710098324160533	27.35167893142044	23.83996042297941
47	24.41621019277648	25.028909669496002	27.53276532274322	23.0221148149843
48	23.86497854809765	28.26172186609907	24.99873230326274	22.87456728254054
49	22.742850499596194	26.088580464113054	27.836146795571842	23.332422240718913
50	22.662488003245308	28.33583987177077	24.662359378246546	24.339312746737377
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	12.0
1	14.5
2	17.0
3	91.5
4	166.0
5	170.0
6	174.0
7	165.0
8	156.0
9	238.5
10	321.0
11	467.5
12	614.0
13	1026.0
14	1438.0
15	2009.5
16	2581.0
17	3120.0
18	3659.0
19	3890.5
20	4122.0
21	4843.0
22	5564.0
23	5924.0
24	6284.0
25	7101.0
26	7918.0
27	10039.0
28	12160.0
29	13701.5
30	15243.0
31	16278.0
32	17313.0
33	19868.0
34	22423.0
35	25733.5
36	29044.0
37	32954.5
38	36865.0
39	38287.5
40	39710.0
41	42450.0
42	45190.0
43	45607.0
44	46024.0
45	54531.5
46	63039.0
47	70111.5
48	77184.0
49	72720.0
50	68256.0
51	59801.0
52	51346.0
53	47871.0
54	44396.0
55	45075.0
56	45754.0
57	43833.5
58	41913.0
59	37728.5
60	33544.0
61	29332.0
62	25120.0
63	21501.5
64	17883.0
65	15244.5
66	12606.0
67	10975.0
68	9344.0
69	8804.5
70	8265.0
71	6235.5
72	4206.0
73	3955.0
74	3704.0
75	2547.5
76	1391.0
77	1281.0
78	1171.0
79	1022.0
80	873.0
81	718.0
82	563.0
83	512.0
84	461.0
85	390.0
86	319.0
87	238.5
88	158.0
89	93.5
90	29.0
91	20.0
92	11.0
93	10.5
94	10.0
95	8.0
96	6.0
97	3.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.020406825526588856
2	0.0037103319139252466
3	6.183886523208744E-4
4	3.7103319139252466E-4
5	0.0
6	3.7103319139252466E-4
7	1.2367773046417487E-4
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	3.7103319139252466E-4
47	0.0
48	0.0
49	0.0
50	1.2367773046417487E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	808553.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.43887160688836
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.4865580450838	46.55448258800629
2	11.509464863330702	12.991624193710289
3	2.9388617412191986	4.975981214492002
4	1.0410941371515026	2.350327133495115
5	0.5033988431928536	1.4205631339008797
6	0.27142503712534727	0.9191353692727431
7	0.17651279222587038	0.6973527972186483
8	0.12237129184538972	0.5525198103064812
9	0.09173329548427343	0.46595913173222947
>10	0.6343020077343382	7.285952045183776
>50	0.10696716351245629	4.239755182622538
>100	0.10630601829702503	11.609783301931865
>500	0.009023906313768239	3.412179682606674
>1k	0.0017607622075645343	1.8844066323908129
>5k	2.2009527594556678E-4	0.6399777831296948
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	5152	0.6371876673514291	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	4822	0.5963740162982514	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	2334	0.28866382290338416	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	1926	0.23820330887400085	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	1338	0.16548080336106602	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	1320	0.16325460421271085	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	1206	0.1491553429397949	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	1199	0.1482895988265457	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	1025	0.12676967372577927	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	990	0.12244095315953314	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	960	0.11873062124560789	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	939	0.11613338890586022	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	893	0.11044421330450818	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	871	0.10772330323429632	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	844	0.1043840045117636	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	809	0.10005528394551749	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.236777304641749E-4	0.0
8	0.0	0.0	0.0	1.236777304641749E-4	0.0
9	0.0	0.0	0.0	1.236777304641749E-4	0.0
10	1.236777304641749E-4	0.0	0.0	1.236777304641749E-4	0.0
11	1.236777304641749E-4	0.0	0.0	2.473554609283498E-4	0.0
12	1.236777304641749E-4	0.0	0.0	2.473554609283498E-4	0.0
13	1.236777304641749E-4	0.0	0.0	2.473554609283498E-4	0.0
14	1.236777304641749E-4	0.0	0.0	2.473554609283498E-4	0.0
15	1.236777304641749E-4	0.0	0.0	2.473554609283498E-4	0.0
16	1.236777304641749E-4	0.0	0.0	2.473554609283498E-4	0.0
17	1.236777304641749E-4	0.0	0.0	2.473554609283498E-4	0.0
18	1.236777304641749E-4	0.0	0.0	4.947109218566996E-4	0.0
19	1.236777304641749E-4	0.0	0.0	9.894218437133992E-4	0.0
20	1.236777304641749E-4	0.0	0.0	9.894218437133992E-4	0.0
21	1.236777304641749E-4	0.0	0.0	0.001236777304641749	0.0
22	1.236777304641749E-4	0.0	0.0	0.0017314882264984485	0.0
23	1.236777304641749E-4	0.0	0.0	0.002597232339747673	0.0
24	1.236777304641749E-4	0.0	0.0	0.004081365105317771	0.0
25	1.236777304641749E-4	0.0	0.0	0.005194464679495346	0.0
26	1.236777304641749E-4	0.0	0.0	0.006183886523208744	0.0
27	1.236777304641749E-4	0.0	0.0	0.009152152054348942	0.0
28	1.236777304641749E-4	0.0	0.0	0.014346616733844287	0.0
29	1.236777304641749E-4	0.0	0.0	0.02201463602262313	0.0
30	1.236777304641749E-4	0.0	0.0	0.034011375877648096	0.0
31	1.236777304641749E-4	0.0	0.0	0.05948898835326812	0.0
32	1.236777304641749E-4	0.0	0.0	0.09152152054348942	0.0
33	1.236777304641749E-4	0.0	0.0	0.1266459959953151	0.0
34	1.236777304641749E-4	0.0	0.0	0.16783068023988532	0.0
35	1.236777304641749E-4	0.0	0.0	0.21297305185930915	0.0
36	1.236777304641749E-4	0.0	0.0	0.2826036141106396	0.0
37	1.236777304641749E-4	0.0	0.0	0.38686394089193904	0.0
38	1.236777304641749E-4	0.0	0.0	0.5081917944772947	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTAGAC	50	0.0025798318	21.999815	3
CTATACT	105	7.7388904E-8	20.952204	4
GTATAGC	150	2.1827873E-11	20.53824	1
CGCCCCT	190	0.0	19.684044	5
CGGTCCA	295	0.0	19.389668	10
AATAGAG	80	8.984462E-5	19.249838	5
CCTATAT	70	8.107109E-4	18.86165	1
GTATAGG	105	1.7870989E-6	18.861649	1
GTGTATA	370	0.0	18.436838	1
GATATAC	275	0.0	18.404396	1
AACGTGG	60	0.0074103926	18.33318	5
CATTCGA	180	1.4551915E-11	18.33318	41
TGGTATA	265	0.0	18.263996	44
TAACGGC	145	6.2991603E-9	18.206743	36
ACTATAC	85	1.4301829E-4	18.117495	3
CGCAATA	270	0.0	17.925776	36
TTAGGAC	260	0.0	17.769081	3
TATATCA	265	0.0	17.433815	4
TAGCGGC	280	0.0	17.285568	30
CTAGCGG	280	0.0	17.285568	29
>>END_MODULE