FastQCFastQC Report
Thu 2 Feb 2017
SRR4062464_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062464_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1924990
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT42150.21896217642689053No Hit
GTACTTTTTTTTTTTTTTTTTTTTT29780.15470210234858364No Hit
TATCAACGCAGAGTACTTTTTTTTT28620.1486760970186858No Hit
GATTAAGAGGGACGGCCGGGGGCAT27880.14483192120478547No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG25650.133247445441275No Hit
GAATAACGCCGCCGCATCGCCAGTC25330.13158509914337216No Hit
GAATAGGACCGCGGTTCTATTTTGT25270.1312734092125154No Hit
GCGCAAGACGGACCAGAGCGAAAGC25250.13116951256889645No Hit
GTCTTGCGCCGGTCCAAGAATTTCA25110.13044223606356395No Hit
GTATCTGATCGTCTTCGAACCTCCG23820.12374090255014311No Hit
GAACTACGACGGTATCTGATCGTCT23470.12192271128681187No Hit
CTATTGGAGCTGGAATTACCGCGGC23080.11989672673624277No Hit
GTCCTATTCCATTATTCCTAGCTGC22770.1182863287601494No Hit
GAGTACTTTTTTTTTTTTTTTTTTT21140.10981875230520678No Hit
GTACATGGAAGCAGTGGTATCAACG20620.10711743957111466No Hit
CTCTTAATCATGGCCTCAGTTCCGA20430.10613042145673483No Hit
ATCAGATACCGTCGTAGTTCCGACC20270.10529924830778342No Hit
CTTTAATATACGCTATTGGAGCTGG20120.10452002348064146No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG20100.10441612683702253No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACCG400.00528061714.2496295
TGCCGTA400.00528061714.2496295
CGTTATT3200.014.2403682
GCGTTAT3250.014.0190981
AATCGCT1850.013.86630815
CGTCTTA707.256882E-613.57284115
ATCGCTC2000.012.35257116
CAAATCG2000.012.35160613
GCGGTAC701.0879841E-412.218119
TCAATAC1405.2750693E-1112.2079343
GTTATTC4050.012.1911883
ACGCTTA550.00306232912.09437117
TCGACGC550.003065911612.09248314
AAATCGC2050.012.05034814
CGCATCG3800.012.00156113
TTAGGAC1900.011.993763
CGGTCCA3350.011.91199810
CGCGCCA802.8633749E-511.87654410
TAGTACG802.8870963E-511.8669732
ATCGCCA3800.011.75244516
Produced by FastQC (version 0.11.5)