##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062464_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1924990
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.168422173621682	32.0	32.0	32.0	32.0	32.0
2	30.840732679130802	32.0	32.0	32.0	32.0	32.0
3	30.83171652839755	32.0	32.0	32.0	32.0	32.0
4	30.89686959412776	32.0	32.0	32.0	32.0	32.0
5	30.725498314276958	32.0	32.0	32.0	32.0	32.0
6	34.36169434646414	36.0	36.0	36.0	32.0	36.0
7	34.241817879573404	36.0	36.0	36.0	32.0	36.0
8	34.237893703343914	36.0	36.0	36.0	32.0	36.0
9	34.37402480012883	36.0	36.0	36.0	32.0	36.0
10	34.073424277528716	36.0	36.0	36.0	32.0	36.0
11	34.37254790933979	36.0	36.0	36.0	32.0	36.0
12	34.15297170374911	36.0	36.0	36.0	32.0	36.0
13	34.279023267653336	36.0	36.0	36.0	32.0	36.0
14	34.182530298858694	36.0	36.0	36.0	32.0	36.0
15	34.137382531857305	36.0	36.0	36.0	32.0	36.0
16	34.1257061075642	36.0	36.0	36.0	32.0	36.0
17	34.05628029236515	36.0	36.0	36.0	32.0	36.0
18	34.06699203632227	36.0	36.0	36.0	32.0	36.0
19	34.049971168681395	36.0	36.0	36.0	32.0	36.0
20	34.041179434698364	36.0	36.0	36.0	32.0	36.0
21	34.01724944025683	36.0	36.0	36.0	32.0	36.0
22	33.96438526953387	36.0	36.0	36.0	32.0	36.0
23	33.93606927828196	36.0	36.0	36.0	32.0	36.0
24	33.90942550350911	36.0	36.0	36.0	32.0	36.0
25	33.46950425716497	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	12.0
4	57.0
5	175.0
6	312.0
7	86.0
8	291.0
9	336.0
10	199.0
11	71.0
12	137.0
13	111.0
14	378.0
15	450.0
16	719.0
17	943.0
18	1338.0
19	1917.0
20	2819.0
21	4030.0
22	6112.0
23	8814.0
24	11884.0
25	16615.0
26	22767.0
27	29200.0
28	39342.0
29	51784.0
30	66135.0
31	90440.0
32	129826.0
33	189250.0
34	427950.0
35	820490.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.148850127440355	17.46551109661049	11.1150018027641	26.270636973185052
2	17.268665710782784	20.406718201275897	36.29646946366602	26.028146624275294
3	19.05273247251163	24.24400165202958	28.123154129684323	28.580111745774467
4	12.896620771028026	15.371098996274279	35.63758846050535	36.09469177219234
5	15.078774487438382	36.22841743255139	32.94658882929025	15.746219250719982
6	33.879930234056836	35.20488028987375	17.05419749416365	13.860991981905757
7	30.090100111812923	30.361705191269756	21.117723777894103	18.43047091902322
8	28.364919928228904	31.863630762575667	19.96280423035589	19.808645078839536
9	27.98523100345359	14.121332059080146	18.69216074308789	39.20127619437838
10	16.29327287885336	26.357872422532296	30.746803178124882	26.60205152048946
11	37.80748735412911	21.069482338427743	21.871973754422026	19.251056553021122
12	24.956371798106105	23.81072512542295	28.370216827612037	22.862686248858903
13	29.63391166864299	19.233289646133684	25.11306663338118	26.019732051842144
14	23.909709129208004	19.77063206210098	24.836750880934115	31.482907927756905
15	25.531346146245266	26.815584350745663	22.20384096123883	25.449228541770246
16	26.064947329883008	25.53798613769117	23.525101512705728	24.87196501972009
17	24.300139181595576	25.39523204214144	25.106570661185394	25.198058115077593
18	25.270266334052515	24.317156190638674	26.111025576474557	24.301551898834255
19	25.844523600471053	24.759688406370024	25.263510505049663	24.132277488109256
20	26.037183568580318	23.77540071908135	24.765814874779675	25.42160083755866
21	27.34071370910695	23.925608142408937	23.934606486297554	24.799071662186567
22	26.200352207016568	24.21217741452997	24.552106608953967	25.035363769499497
23	24.48482647909187	23.849098140724795	25.201652966506828	26.46442241367651
24	24.77240217012489	24.95009024848242	24.99893366346419	25.278573917928497
25	24.875099933057204	24.3914681485974	25.024225801636067	25.709206116709325
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	280.0
1	280.0
2	759.5
3	1239.0
4	1239.0
5	1239.0
6	2979.5
7	4720.0
8	4720.0
9	4720.0
10	4945.0
11	5170.0
12	5170.0
13	5170.0
14	5613.5
15	6057.0
16	6057.0
17	6057.0
18	9734.5
19	13412.0
20	13412.0
21	13412.0
22	21469.5
23	29527.0
24	29527.0
25	29527.0
26	43274.0
27	57021.0
28	57021.0
29	57021.0
30	69629.0
31	82237.0
32	82237.0
33	82237.0
34	102363.5
35	122490.0
36	122490.0
37	122490.0
38	137352.0
39	152214.0
40	152214.0
41	152214.0
42	173326.5
43	194439.0
44	194439.0
45	194439.0
46	219668.0
47	244897.0
48	244897.0
49	244897.0
50	245426.0
51	245955.0
52	245955.0
53	245955.0
54	226163.5
55	206372.0
56	206372.0
57	206372.0
58	192318.5
59	178265.0
60	178265.0
61	178265.0
62	161985.5
63	145706.0
64	145706.0
65	145706.0
66	123959.0
67	102212.0
68	102212.0
69	102212.0
70	78932.0
71	55652.0
72	55652.0
73	55652.0
74	45258.5
75	34865.0
76	34865.0
77	34865.0
78	29238.0
79	23611.0
80	23611.0
81	23611.0
82	17112.5
83	10614.0
84	10614.0
85	10614.0
86	7873.5
87	5133.0
88	5133.0
89	5133.0
90	3453.5
91	1774.0
92	1774.0
93	1774.0
94	1024.5
95	275.0
96	275.0
97	275.0
98	564.0
99	853.0
100	853.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008727318063989943
2	0.0036363825266624763
3	0.004415607353804436
4	0.01355851199227009
5	0.03303913267081907
6	0.04374048696356864
7	0.06446786736554476
8	0.0858705759510439
9	0.09205242624637011
10	0.11537722273881942
11	0.11574086099148566
12	0.12961106291461255
13	0.1158447576351046
14	0.12026036498890902
15	0.11106551202863392
16	0.10794861272006608
17	0.12062400324157527
18	0.12685780185871096
19	0.12914352801832737
20	0.11735125896757904
21	0.12540324884804596
22	0.14675400911173564
23	0.13652018971527125
24	0.13090977095984915
25	0.12742923339861506
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1924990.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.68762084409706
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.0960422851361	39.30094159332511
2	13.048731381081	12.967208347192475
3	3.741209607694513	5.576754134562542
4	1.3955505564046147	2.773663476616047
5	0.698865847003835	1.7362490613407653
6	0.4034359798263384	1.2027464400286736
7	0.24989084232672365	0.86915369981596
8	0.1939627812010905	0.7710039304149074
9	0.13923883635585807	0.6226601856860837
>10	0.8107973141015742	7.565628745516125
>50	0.09338039748698389	3.2690920610230196
>100	0.10370398788242866	11.06317801133008
>500	0.017318251155544813	5.986856249632594
>1k	0.00787193234342946	6.294864063515623
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4215	0.21896217642689053	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2978	0.15470210234858364	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2862	0.1486760970186858	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2788	0.14483192120478547	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2565	0.133247445441275	No Hit
GAATAACGCCGCCGCATCGCCAGTC	2533	0.13158509914337216	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2527	0.1312734092125154	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2525	0.13116951256889645	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2511	0.13044223606356395	No Hit
GTATCTGATCGTCTTCGAACCTCCG	2382	0.12374090255014311	No Hit
GAACTACGACGGTATCTGATCGTCT	2347	0.12192271128681187	No Hit
CTATTGGAGCTGGAATTACCGCGGC	2308	0.11989672673624277	No Hit
GTCCTATTCCATTATTCCTAGCTGC	2277	0.1182863287601494	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2114	0.10981875230520678	No Hit
GTACATGGAAGCAGTGGTATCAACG	2062	0.10711743957111466	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	2043	0.10613042145673483	No Hit
ATCAGATACCGTCGTAGTTCCGACC	2027	0.10529924830778342	No Hit
CTTTAATATACGCTATTGGAGCTGG	2012	0.10452002348064146	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG	2010	0.10441612683702253	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	5.194832180946394E-5	0.0
6	0.0	0.0	0.0	5.194832180946394E-5	0.0
7	0.0	0.0	0.0	1.0389664361892788E-4	0.0
8	0.0	0.0	0.0	1.0389664361892788E-4	0.0
9	0.0	0.0	0.0	2.0779328723785577E-4	5.194832180946394E-5
10	0.0	0.0	0.0	2.0779328723785577E-4	5.194832180946394E-5
11	0.0	0.0	0.0	2.597416090473197E-4	5.194832180946394E-5
12	0.0	0.0	0.0	2.597416090473197E-4	5.194832180946394E-5
13	0.0	0.0	0.0	2.597416090473197E-4	5.194832180946394E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAACCG	40	0.005280617	14.249629	5
TGCCGTA	40	0.005280617	14.249629	5
CGTTATT	320	0.0	14.240368	2
GCGTTAT	325	0.0	14.019098	1
AATCGCT	185	0.0	13.866308	15
CGTCTTA	70	7.256882E-6	13.572841	15
ATCGCTC	200	0.0	12.352571	16
CAAATCG	200	0.0	12.351606	13
GCGGTAC	70	1.0879841E-4	12.2181	19
TCAATAC	140	5.2750693E-11	12.207934	3
GTTATTC	405	0.0	12.191188	3
ACGCTTA	55	0.003062329	12.094371	17
TCGACGC	55	0.0030659116	12.092483	14
AAATCGC	205	0.0	12.050348	14
CGCATCG	380	0.0	12.001561	13
TTAGGAC	190	0.0	11.99376	3
CGGTCCA	335	0.0	11.911998	10
CGCGCCA	80	2.8633749E-5	11.876544	10
TAGTACG	80	2.8870963E-5	11.866973	2
ATCGCCA	380	0.0	11.752445	16
>>END_MODULE