FastQCFastQC Report
Thu 2 Feb 2017
SRR4062464_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062464_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1924990
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA28050.14571504267554636No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC25950.13480589509555893No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT25050.1301305461327072No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT23970.12452012737728507No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA23770.1234811609410958No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG23370.12140322806871724No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA22840.11864996701281565No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC22490.1168317757494844No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA22390.11631229253138978No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG21280.11054602881053928No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA20420.10607847313492537No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT19520.10140312417207362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACG1705.456968E-1219.4121762
CGTCGTA6100.018.03270110
TAGGACG2055.456968E-1217.170654
CTAGGAC2450.017.0611443
ATACCGT6650.016.87216
GTATTAA2251.8189894E-1216.6251661
GTATTAT1602.1267624E-816.5029241
CGCGCGA800.00198898916.49992210
CAAGACG9200.016.4999224
GTCGTAG6550.016.45793711
GTCCTAT8050.016.1270791
CGCAATA7400.015.75668236
TACGCCT2101.5097612E-1015.7142115
GTATCAA48200.015.6127231
CCGTCGT6900.015.3042769
ACCGTCG7400.015.162098
AAGACGG9850.014.9643965
TAATAGT2651.8189894E-1214.9433264
TACCGTC7150.014.769167
CTATTCC8050.014.7576944
Produced by FastQC (version 0.11.5)