##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062464_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1924990 Sequences flagged as poor quality 0 Sequence length 50 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.278517810482132 32.0 32.0 32.0 32.0 32.0 2 31.434365373326614 32.0 32.0 32.0 32.0 32.0 3 31.512883183808746 32.0 32.0 32.0 32.0 32.0 4 31.607982379129243 32.0 32.0 32.0 32.0 32.0 5 31.55002155855355 32.0 32.0 32.0 32.0 32.0 6 35.146775307923676 36.0 36.0 36.0 36.0 36.0 7 35.16140187741235 36.0 36.0 36.0 36.0 36.0 8 35.10036259928623 36.0 36.0 36.0 36.0 36.0 9 35.226283253419496 36.0 36.0 36.0 36.0 36.0 10 35.10221144005943 36.0 36.0 36.0 36.0 36.0 11 35.22854300541821 36.0 36.0 36.0 36.0 36.0 12 35.13068483472642 36.0 36.0 36.0 36.0 36.0 13 35.17347155050156 36.0 36.0 36.0 36.0 36.0 14 35.13689473711552 36.0 36.0 36.0 36.0 36.0 15 35.09966753074042 36.0 36.0 36.0 36.0 36.0 16 35.116034888492926 36.0 36.0 36.0 36.0 36.0 17 35.085114727868714 36.0 36.0 36.0 36.0 36.0 18 35.0972410246287 36.0 36.0 36.0 36.0 36.0 19 35.076006109122645 36.0 36.0 36.0 36.0 36.0 20 35.06975464807609 36.0 36.0 36.0 36.0 36.0 21 35.06558371731801 36.0 36.0 36.0 36.0 36.0 22 35.049891687749025 36.0 36.0 36.0 36.0 36.0 23 35.01164473581681 36.0 36.0 36.0 36.0 36.0 24 34.987402012477986 36.0 36.0 36.0 36.0 36.0 25 34.9592184894467 36.0 36.0 36.0 32.0 36.0 26 34.92279388464355 36.0 36.0 36.0 32.0 36.0 27 34.908813032794974 36.0 36.0 36.0 32.0 36.0 28 34.882576013381886 36.0 36.0 36.0 32.0 36.0 29 34.86224707660819 36.0 36.0 36.0 32.0 36.0 30 34.84694413996956 36.0 36.0 36.0 32.0 36.0 31 34.85023662460584 36.0 36.0 36.0 32.0 36.0 32 34.808934072384794 36.0 36.0 36.0 32.0 36.0 33 34.79097294011917 36.0 36.0 36.0 32.0 36.0 34 34.772518298796356 36.0 36.0 36.0 32.0 36.0 35 34.738803837942015 36.0 36.0 36.0 32.0 36.0 36 34.71397150115065 36.0 36.0 36.0 32.0 36.0 37 34.704873272068944 36.0 36.0 36.0 32.0 36.0 38 34.66992503857163 36.0 36.0 36.0 32.0 36.0 39 34.660334339399164 36.0 36.0 36.0 32.0 36.0 40 34.64571296474267 36.0 36.0 36.0 32.0 36.0 41 34.6112109673297 36.0 36.0 36.0 32.0 36.0 42 34.578996254526 36.0 36.0 36.0 32.0 36.0 43 34.570005558470434 36.0 36.0 36.0 32.0 36.0 44 34.53701681567177 36.0 36.0 36.0 32.0 36.0 45 34.48844825167923 36.0 36.0 36.0 32.0 36.0 46 34.48864981116785 36.0 36.0 36.0 32.0 36.0 47 34.45887822793885 36.0 36.0 36.0 32.0 36.0 48 34.41128161704736 36.0 36.0 36.0 32.0 36.0 49 34.39560724990779 36.0 36.0 36.0 32.0 36.0 50 33.872107907054065 36.0 36.0 36.0 32.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 5.0 20 10.0 21 41.0 22 162.0 23 483.0 24 1288.0 25 3018.0 26 6255.0 27 11351.0 28 19409.0 29 29608.0 30 42728.0 31 60996.0 32 89778.0 33 148456.0 34 329758.0 35 1181644.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.59740114667518 18.108063875055528 11.60152647562341 26.693008502645878 2 16.65707318441617 20.918085861319806 36.053483836714086 26.37135711754994 3 18.3782551055182 24.603527524499004 28.504043686778434 28.514173683204362 4 12.632053045635704 15.793274125914813 35.842583626669104 35.732089201780376 5 14.687089283580693 36.42902041049564 32.96396344916078 15.919926856762892 6 34.00627538987418 35.393406684744775 16.80209664515995 13.798221280221092 7 30.026909230697303 30.409248879214957 21.09704465997226 18.466797230115482 8 28.31001719489452 32.01699749089605 19.84799921038551 19.824986103823917 9 28.098899215060857 13.861370708419265 18.643785162520324 39.39594491399955 10 16.290837874482463 26.44050098961553 30.784263814357477 26.484397321544527 11 38.0332365362937 20.817510740315534 21.95206208863423 19.197190634756545 12 24.92786975516756 23.768279315736702 28.35484859661609 22.949002332479647 13 29.801349617400607 19.07085231611593 25.18766331253669 25.940134753946776 14 23.99835843303082 19.584465373846097 24.85186936036031 31.56530683276277 15 25.598210899796882 26.727827157543675 22.14577738066172 25.528184561997726 16 26.176863256432505 25.415820341923855 23.455186780191063 24.952129621452578 17 24.442672429467166 25.41919698284147 25.000233767448144 25.13789682024322 18 25.46174265840342 24.213320588678382 26.10195377638325 24.222982976534944 19 26.07977184297061 24.58864721375176 25.196598423887917 24.134982519389713 20 26.092966716710215 23.661473566096447 24.776232603805735 25.469327113387603 21 27.527519623478565 23.88324095190105 23.943656850165457 24.645582574454934 22 26.300915848913505 24.057215881640946 24.59243944124385 25.0494288282017 23 24.49088047210635 23.722460895900756 25.289897609857714 26.49676102213518 24 24.904233268744253 24.74376490267482 24.950727016763725 25.401274811817203 25 25.086571878295473 24.105891459176412 25.024389737089543 25.783146925438572 26 24.837583571862712 25.080390028000142 25.406209902389104 24.67581649774804 27 25.90590080987434 24.233268744253216 24.589997870118808 25.270832575753637 28 24.620283741733722 24.31700943901007 25.62169154125476 25.441015278001444 29 24.628179886648763 24.647348817396452 25.25805328858851 25.466418007366272 30 24.629894181268476 24.777063776954687 25.68563992540221 24.90740211637463 31 25.353482355752497 24.611452527026113 24.302360012259804 25.732705104961585 32 25.0499483114198 24.52012737728508 24.47846482319389 25.951459488101236 33 24.697842586195254 23.929942493207758 25.226832347181023 26.145382573415965 34 25.377898067002945 24.34620439586699 25.345586210837457 24.930311326292603 35 26.30595483612902 24.039709297191152 25.070675691821776 24.583660174858053 36 24.809947064660076 24.88828513394875 24.786882009776672 25.5148857916145 37 25.965173845058935 24.70895952706248 24.48308822383493 24.84277840404366 38 24.89685660704731 24.25597016088395 25.301222344012174 25.545950888056563 39 25.42906716398527 24.12267076712087 24.70594652439753 25.742315544496336 40 25.84938103574564 24.317840612159024 25.17426064550985 24.65851770658549 41 24.434360697977652 24.85347975833641 25.691510085766676 25.020649457919262 42 25.996135044857375 24.9363373316225 25.08864981116785 23.978877812352273 43 25.10558496407774 23.654720284261217 25.332287440454238 25.90740731120681 44 24.635140961771228 24.582205621847386 24.929324308178224 25.85332910820316 45 24.71680372365571 24.810258754590933 25.013584486153174 25.459353035600184 46 24.32752827550094 24.368048008610963 25.36275550808628 25.941668207801815 47 24.547672739948123 24.075462249394672 25.92461567312852 25.45224933752868 48 25.5796653489109 24.928804824960128 24.671712580325092 24.819817245803875 49 24.299243112951235 24.97592195284131 25.157169647634532 25.567665286572915 50 24.3522823495187 25.412651494293474 24.76028446901023 25.474781687177593 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 31.0 1 37.0 2 43.0 3 108.0 4 173.0 5 208.5 6 244.0 7 264.5 8 285.0 9 396.0 10 507.0 11 725.5 12 944.0 13 1654.5 14 2365.0 15 3339.5 16 4314.0 17 5282.5 18 6251.0 19 6788.5 20 7326.0 21 8237.5 22 9149.0 23 10065.0 24 10981.0 25 12141.0 26 13301.0 27 15770.0 28 18239.0 29 21292.0 30 24345.0 31 27463.5 32 30582.0 33 35112.5 34 39643.0 35 45330.5 36 51018.0 37 61322.0 38 71626.0 39 77264.5 40 82903.0 41 90666.0 42 98429.0 43 100417.0 44 102405.0 45 112815.5 46 123226.0 47 130592.0 48 137958.0 49 142139.5 50 146321.0 51 137020.5 52 127720.0 53 124416.5 54 121113.0 55 125233.5 56 129354.0 57 128009.0 58 126664.0 59 115346.0 60 104028.0 61 91851.5 62 79675.0 63 71173.5 64 62672.0 65 54599.0 66 46526.0 67 43039.0 68 39552.0 69 38060.0 70 36568.0 71 29352.0 72 22136.0 73 19896.0 74 17656.0 75 13143.5 76 8631.0 77 7914.5 78 7198.0 79 6215.0 80 5232.0 81 4076.5 82 2921.0 83 2588.0 84 2255.0 85 1867.5 86 1480.0 87 1097.5 88 715.0 89 448.5 90 182.0 91 120.5 92 59.0 93 40.0 94 21.0 95 16.0 96 11.0 97 10.0 98 9.0 99 6.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015324754933791864 2 0.0037402791702814044 3 7.272765053324953E-4 4 3.1168993085678367E-4 5 0.0 6 5.194832180946394E-4 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 1.038966436189279E-4 47 5.194832180946395E-5 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 1924990.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.94157150763188 #Duplication Level Percentage of deduplicated Percentage of total 1 79.76987693431965 42.23142643873279 2 12.878864663303204 13.6365466901876 3 3.49087178745972 5.544367150793204 4 1.337950327904966 2.8333277183376104 5 0.6472053495943512 1.7132034147835613 6 0.371196246255921 1.1791027568713424 7 0.2635712355531906 0.9767712790075888 8 0.16965564614779646 0.7185469217765645 9 0.13010979470852171 0.6199395300364056 >10 0.7505918764748318 7.406734694696722 >50 0.08001993840848855 2.994033068256265 >100 0.08984731244259063 10.156580092023635 >500 0.014117858058677976 5.15940420869794 >1k 0.006121029368098143 4.830016035798856 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA 2805 0.14571504267554636 No Hit GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC 2595 0.13480589509555893 No Hit GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT 2505 0.1301305461327072 No Hit GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT 2397 0.12452012737728507 No Hit GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA 2377 0.1234811609410958 No Hit GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG 2337 0.12140322806871724 No Hit GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA 2284 0.11864996701281565 No Hit GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC 2249 0.1168317757494844 No Hit CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA 2239 0.11631229253138978 No Hit ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG 2128 0.11054602881053928 No Hit CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA 2042 0.10607847313492537 No Hit GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT 1952 0.10140312417207362 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.194832180946394E-5 0.0 5 0.0 0.0 0.0 1.0389664361892788E-4 0.0 6 0.0 0.0 0.0 1.0389664361892788E-4 0.0 7 0.0 0.0 0.0 1.0389664361892788E-4 0.0 8 0.0 0.0 0.0 1.0389664361892788E-4 0.0 9 0.0 0.0 0.0 2.0779328723785577E-4 0.0 10 0.0 0.0 0.0 2.0779328723785577E-4 0.0 11 0.0 0.0 0.0 2.597416090473197E-4 0.0 12 0.0 0.0 0.0 2.597416090473197E-4 0.0 13 0.0 0.0 0.0 2.597416090473197E-4 0.0 14 0.0 0.0 0.0 2.597416090473197E-4 0.0 15 0.0 0.0 0.0 2.597416090473197E-4 0.0 16 0.0 0.0 0.0 2.597416090473197E-4 5.194832180946394E-5 17 0.0 0.0 0.0 3.1168993085678367E-4 5.194832180946394E-5 18 0.0 0.0 0.0 3.1168993085678367E-4 5.194832180946394E-5 19 0.0 0.0 0.0 3.1168993085678367E-4 5.194832180946394E-5 20 0.0 0.0 0.0 5.194832180946394E-4 5.194832180946394E-5 21 0.0 0.0 0.0 5.714315399041034E-4 5.194832180946394E-5 22 0.0 0.0 0.0 9.87018114379815E-4 5.194832180946394E-5 23 0.0 0.0 0.0 0.001766242941521774 5.194832180946394E-5 24 0.0 0.0 0.0 0.0032727442739962287 5.194832180946394E-5 25 0.0 0.0 0.0 0.003844175813900332 5.194832180946394E-5 26 0.0 0.0 0.0 0.0048831422500896105 5.194832180946394E-5 27 0.0 0.0 0.0 0.007168868409706024 5.194832180946394E-5 28 0.0 0.0 0.0 0.013922150244936338 5.194832180946394E-5 29 0.0 0.0 0.0 0.02857157699520517 5.194832180946394E-5 30 0.0 0.0 0.0 0.05527301440526964 5.194832180946394E-5 31 0.0 0.0 0.0 0.12197465960862135 5.194832180946394E-5 32 0.0 0.0 0.0 0.19662439804882104 5.194832180946394E-5 33 0.0 0.0 0.0 0.27428713915396963 5.194832180946394E-5 34 0.0 0.0 0.0 0.3603655083922514 5.194832180946394E-5 35 0.0 0.0 0.0 0.45771666346318685 5.194832180946394E-5 36 0.0 0.0 0.0 0.5954836129018852 5.194832180946394E-5 37 0.0 0.0 0.0 0.7919002176634684 5.194832180946394E-5 38 0.0 0.0 0.0 1.0536678112613571 5.194832180946394E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGTACG 170 5.456968E-12 19.412176 2 CGTCGTA 610 0.0 18.032701 10 TAGGACG 205 5.456968E-12 17.17065 4 CTAGGAC 245 0.0 17.061144 3 ATACCGT 665 0.0 16.8721 6 GTATTAA 225 1.8189894E-12 16.625166 1 GTATTAT 160 2.1267624E-8 16.502924 1 CGCGCGA 80 0.001988989 16.499922 10 CAAGACG 920 0.0 16.499922 4 GTCGTAG 655 0.0 16.457937 11 GTCCTAT 805 0.0 16.127079 1 CGCAATA 740 0.0 15.756682 36 TACGCCT 210 1.5097612E-10 15.714211 5 GTATCAA 4820 0.0 15.612723 1 CCGTCGT 690 0.0 15.304276 9 ACCGTCG 740 0.0 15.16209 8 AAGACGG 985 0.0 14.964396 5 TAATAGT 265 1.8189894E-12 14.943326 4 TACCGTC 715 0.0 14.76916 7 CTATTCC 805 0.0 14.757694 4 >>END_MODULE