FastQCFastQC Report
Thu 2 Feb 2017
SRR4062463_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062463_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1218386
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG144481.1858310912961902No Hit
TCCATGTACTCTGCGTTGATACCAC124921.0252908355808423No Hit
GAGTACATGGAAGCAGTGGTATCAA61520.5049302930270047No Hit
CATGTACTCTGCGTTGATACCACTG53670.4405007936729411No Hit
GCTTCCATGTACTCTGCGTTGATAC39180.321572966202829No Hit
GTATCAACGCAGAGTACTTTTTTTT38750.3180437070025427No Hit
GCGTTGATACCACTGCTTCCATGTA35510.2914511493073623No Hit
GTACTTTTTTTTTTTTTTTTTTTTT34230.280945447501859No Hit
CATGGAAGCAGTGGTATCAACGCAG33070.27142465524062165No Hit
CCCATGTACTCTGCGTTGATACCAC32630.26781332024497984No Hit
TATCAACGCAGAGTACTTTTTTTTT29550.2425339752754874No Hit
GTACATGGGAAGCAGTGGTATCAAC29140.2391688676659121No Hit
GTATCAACGCAGAGTACATGGAAGC26590.2182395398502609No Hit
ACGCAGAGTACATGGAAGCAGTGGT23280.19107245158759212No Hit
GAGTACTTTTTTTTTTTTTTTTTTT23130.18984131465725967No Hit
ACTCTGCGTTGATACCACTGCTTCC21520.176627111605025No Hit
CAGTGGTATCAACGCAGAGTACATG19460.15971949776179306No Hit
TATCAACGCAGAGTACATGGAAGCA18220.14954209913771171No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17120.14051376164860727No Hit
GCGCAAGACGGACCAGAGCGAAAGC14410.11827122110726813No Hit
GTACATGGGGTGGTATCAACGCAAA14320.11753253894906869No Hit
GGTATCAACGCAGAGTACTTTTTTT13730.11269006702309449No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG13480.11063817213920711No Hit
GTCTTGCGCCGGTCCAAGAATTTCA13400.10998156577636316No Hit
GTATCAACGCAGAGTACATGGGAAG13310.1092428836181637No Hit
GAATAGGACCGCGGTTCTATTTTGT13080.10735514032498732No Hit
ATACCACTGCTTCCATGTACTCTGC12850.10546739703181093No Hit
GAATAACGCCGCCGCATCGCCAGTC12680.10407210851076754No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAAG456.7990745E-414.7667891
GCGTTAT851.7322236E-814.5186071
CGTCGTA1900.014.50053210
ATCGCCA2400.014.25403916
CCGTCGT1900.014.0005149
GTTACTA551.9589902E-413.8186899
CGGTTCT2150.013.69874112
CGCATCG2450.013.57304413
CGTTATT1001.0128133E-813.2939322
TCGCCAG2600.013.15865617
CGCCAGT2600.013.15865618
GCATCGC2600.013.1548714
GTATAAT802.0151456E-613.0527871
CAAGACG2700.013.0157874
ACCGTCG2050.012.9760878
CATCGCC2650.012.90719615
CCAACGA1051.9826984E-812.67077819
GGTTCTA2400.012.66817413
AACCGCG1051.990702E-812.6671337
GTCCTAA2150.012.3628931
Produced by FastQC (version 0.11.5)