##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062463_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1218386
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.994308043592095	32.0	32.0	32.0	32.0	32.0
2	30.69374319796846	32.0	32.0	32.0	32.0	32.0
3	30.693716933713947	32.0	32.0	32.0	32.0	32.0
4	30.7316975080147	32.0	32.0	32.0	32.0	32.0
5	30.573953574647113	32.0	32.0	32.0	32.0	32.0
6	34.17884069580576	36.0	36.0	36.0	32.0	36.0
7	34.01774642847177	36.0	36.0	36.0	32.0	36.0
8	34.00332324895395	36.0	36.0	36.0	32.0	36.0
9	34.14743603422889	36.0	36.0	36.0	32.0	36.0
10	33.849471349802116	36.0	36.0	36.0	32.0	36.0
11	34.17539925770651	36.0	36.0	36.0	32.0	36.0
12	33.95624128970622	36.0	36.0	36.0	32.0	36.0
13	34.06204191446717	36.0	36.0	36.0	32.0	36.0
14	33.96473613452551	36.0	36.0	36.0	32.0	36.0
15	33.926836815262156	36.0	36.0	36.0	32.0	36.0
16	33.910686761010055	36.0	36.0	36.0	32.0	36.0
17	33.81960889241997	36.0	36.0	36.0	32.0	36.0
18	33.843341929404964	36.0	36.0	36.0	32.0	36.0
19	33.84021320008601	36.0	36.0	36.0	32.0	36.0
20	33.829831432731496	36.0	36.0	36.0	32.0	36.0
21	33.75481825956634	36.0	36.0	36.0	32.0	36.0
22	33.71012880975323	36.0	36.0	36.0	27.0	36.0
23	33.64842668907883	36.0	36.0	36.0	27.0	36.0
24	33.63618836723337	36.0	36.0	36.0	27.0	36.0
25	33.1087775138585	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	6.0
4	40.0
5	129.0
6	198.0
7	48.0
8	181.0
9	229.0
10	150.0
11	46.0
12	93.0
13	78.0
14	275.0
15	365.0
16	568.0
17	743.0
18	979.0
19	1587.0
20	2309.0
21	3186.0
22	4936.0
23	6809.0
24	9178.0
25	12607.0
26	16872.0
27	21265.0
28	28272.0
29	36626.0
30	46934.0
31	63315.0
32	89258.0
33	127054.0
34	270893.0
35	473155.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.12739613695893	17.118717386968164	11.670550611813706	25.083335864259194
2	15.475659894331544	21.018041776549875	37.08992324796301	26.416375081155575
3	19.461423612621612	25.343279245323284	28.2228932721454	26.972403869909705
4	12.3748872312533	15.902219748629332	35.44160610605311	36.28128691406427
5	13.721199840389108	37.47087347552715	33.76057709518031	15.04734958890343
6	33.48972980962274	34.70589442827289	17.843567941733145	13.96080782037123
7	28.81880735828694	31.205700553399158	21.61377699442987	18.36171509388403
8	28.454845750741608	32.43148881566571	20.724022269266882	18.389643164325797
9	27.251351677756407	14.935536000512656	18.636434216158353	39.176678105572584
10	15.527898759142083	27.63398800230093	32.02342016599557	24.81469307256143
11	36.75951917640717	21.642896117385646	21.467123341704248	20.13046136450294
12	23.557847986205967	24.388415773720002	29.538577104074925	22.515159135999106
13	30.31664168507416	19.90276901230672	25.312187730093616	24.468401572525508
14	24.017182308500836	19.749053485564577	25.288268809277852	30.94549539665673
15	25.516818637105764	26.731267455647554	24.34074245714713	23.41117145009955
16	25.663110357340063	25.733529883516077	25.202795088217506	23.40056467092635
17	23.600694906702035	25.707603377233422	27.01014254791454	23.68155916815
18	25.0803763387838	24.190237151291612	28.25614574909433	22.473240760830258
19	24.29443072846637	27.37255372517563	25.535114912866096	22.7979006334919
20	24.315584635435926	23.852843095199354	28.07389072692579	23.75768154243893
21	25.598978943587948	25.584678894030883	24.018001625603336	24.79834053677784
22	23.994193229654314	26.008067333546514	25.369273274142202	24.628466162656977
23	22.946697899971234	25.24086631323717	25.390868368059838	26.42156741873176
24	22.97068388916605	27.173763851983594	25.556277908413144	24.299274350437205
25	24.78927713180206	24.993425183519566	24.818288509521977	25.3990091751564
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	334.0
1	334.0
2	742.5
3	1151.0
4	1151.0
5	1151.0
6	2979.5
7	4808.0
8	4808.0
9	4808.0
10	4684.0
11	4560.0
12	4560.0
13	4560.0
14	4358.0
15	4156.0
16	4156.0
17	4156.0
18	6707.5
19	9259.0
20	9259.0
21	9259.0
22	14557.5
23	19856.0
24	19856.0
25	19856.0
26	28837.0
27	37818.0
28	37818.0
29	37818.0
30	47489.0
31	57160.0
32	57160.0
33	57160.0
34	67146.5
35	77133.0
36	77133.0
37	77133.0
38	86020.0
39	94907.0
40	94907.0
41	94907.0
42	113196.0
43	131485.0
44	131485.0
45	131485.0
46	170534.0
47	209583.0
48	209583.0
49	209583.0
50	186530.5
51	163478.0
52	163478.0
53	163478.0
54	141138.0
55	118798.0
56	118798.0
57	118798.0
58	109019.0
59	99240.0
60	99240.0
61	99240.0
62	87826.0
63	76412.0
64	76412.0
65	76412.0
66	63400.0
67	50388.0
68	50388.0
69	50388.0
70	38070.5
71	25753.0
72	25753.0
73	25753.0
74	20365.0
75	14977.0
76	14977.0
77	14977.0
78	12428.5
79	9880.0
80	9880.0
81	9880.0
82	6940.5
83	4001.0
84	4001.0
85	4001.0
86	2987.5
87	1974.0
88	1974.0
89	1974.0
90	1314.5
91	655.0
92	655.0
93	655.0
94	396.0
95	137.0
96	137.0
97	137.0
98	310.0
99	483.0
100	483.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010095322828725871
2	0.00385756238170826
3	0.005170775107396179
4	0.015184022140766555
5	0.03480013723072983
6	0.04440300528732274
7	0.06730215219150582
8	0.09052960227711086
9	0.0980805754498164
10	0.12196463189826541
11	0.12097972235399948
12	0.1336193948387457
13	0.1222929350796874
14	0.12746371018708358
15	0.11269006702309449
16	0.11441365872555988
17	0.1257401184846182
18	0.13214203052234677
19	0.13444015279230065
20	0.1237702993960863
21	0.13189580313628027
22	0.15389211629155294
23	0.14248358073713913
24	0.1381335635832979
25	0.13312694006661271
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1218386.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.96021758212852
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.29181109259709	38.82144323574585
2	12.646248481392083	12.383261544932374
3	3.653165241293287	5.365792952315652
4	1.4810837299364732	2.9005672670016085
5	0.7400456480332238	1.8116397974206968
6	0.42599849411560853	1.2514187376935573
7	0.2917226597838971	0.9997963427659798
8	0.19961761134151806	0.7818657347604391
9	0.15222271855757497	0.6707571679369785
>10	0.8997783584108594	8.355891515099904
>50	0.09892306181105835	3.4163653008426778
>100	0.10074506700443091	10.539601969604167
>500	0.012257315385373776	4.155795631400332
>1k	0.00570888661784532	5.384152537643088
>5k	3.358168598732541E-4	0.9469577836307252
>10k+	3.358168598732541E-4	2.2146924812059847
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	14448	1.1858310912961902	No Hit
TCCATGTACTCTGCGTTGATACCAC	12492	1.0252908355808423	No Hit
GAGTACATGGAAGCAGTGGTATCAA	6152	0.5049302930270047	No Hit
CATGTACTCTGCGTTGATACCACTG	5367	0.4405007936729411	No Hit
GCTTCCATGTACTCTGCGTTGATAC	3918	0.321572966202829	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3875	0.3180437070025427	No Hit
GCGTTGATACCACTGCTTCCATGTA	3551	0.2914511493073623	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3423	0.280945447501859	No Hit
CATGGAAGCAGTGGTATCAACGCAG	3307	0.27142465524062165	No Hit
CCCATGTACTCTGCGTTGATACCAC	3263	0.26781332024497984	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2955	0.2425339752754874	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2914	0.2391688676659121	No Hit
GTATCAACGCAGAGTACATGGAAGC	2659	0.2182395398502609	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	2328	0.19107245158759212	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2313	0.18984131465725967	No Hit
ACTCTGCGTTGATACCACTGCTTCC	2152	0.176627111605025	No Hit
CAGTGGTATCAACGCAGAGTACATG	1946	0.15971949776179306	No Hit
TATCAACGCAGAGTACATGGAAGCA	1822	0.14954209913771171	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1712	0.14051376164860727	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1441	0.11827122110726813	No Hit
GTACATGGGGTGGTATCAACGCAAA	1432	0.11753253894906869	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1373	0.11269006702309449	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1348	0.11063817213920711	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1340	0.10998156577636316	No Hit
GTATCAACGCAGAGTACATGGGAAG	1331	0.1092428836181637	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1308	0.10735514032498732	No Hit
ATACCACTGCTTCCATGTACTCTGC	1285	0.10546739703181093	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1268	0.10407210851076754	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	8.207579535549489E-5	0.0
5	0.0	0.0	0.0	8.207579535549489E-5	0.0
6	0.0	0.0	0.0	8.207579535549489E-5	0.0
7	0.0	0.0	0.0	8.207579535549489E-5	0.0
8	0.0	0.0	0.0	8.207579535549489E-5	0.0
9	0.0	0.0	0.0	8.207579535549489E-5	0.0
10	0.0	0.0	0.0	8.207579535549489E-5	0.0
11	0.0	0.0	0.0	8.207579535549489E-5	0.0
12	0.0	0.0	0.0	8.207579535549489E-5	0.0
13	0.0	0.0	0.0	8.207579535549489E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATAAG	45	6.7990745E-4	14.766789	1
GCGTTAT	85	1.7322236E-8	14.518607	1
CGTCGTA	190	0.0	14.500532	10
ATCGCCA	240	0.0	14.254039	16
CCGTCGT	190	0.0	14.000514	9
GTTACTA	55	1.9589902E-4	13.818689	9
CGGTTCT	215	0.0	13.698741	12
CGCATCG	245	0.0	13.573044	13
CGTTATT	100	1.0128133E-8	13.293932	2
TCGCCAG	260	0.0	13.158656	17
CGCCAGT	260	0.0	13.158656	18
GCATCGC	260	0.0	13.15487	14
GTATAAT	80	2.0151456E-6	13.052787	1
CAAGACG	270	0.0	13.015787	4
ACCGTCG	205	0.0	12.976087	8
CATCGCC	265	0.0	12.907196	15
CCAACGA	105	1.9826984E-8	12.670778	19
GGTTCTA	240	0.0	12.668174	13
AACCGCG	105	1.990702E-8	12.667133	7
GTCCTAA	215	0.0	12.362893	1
>>END_MODULE