FastQCFastQC Report
Thu 2 Feb 2017
SRR4062463_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062463_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1218386
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA75410.618933572775787No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC66870.5488408435421943No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA27760.22784240790685384No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC27050.22201502643661367No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG17650.1448637788024485No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC17120.14051376164860727No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG17080.14018545846718528No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA16960.13920054892291933No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13660.11211553645560603No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13410.11006364157171865No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT13290.10907873202745272No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC13110.1076013677110538No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT12750.104646639078256No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA12540.10292304737579058No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA12500.10259474419436862No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA12470.10234851680830212No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA12440.10210228942223565No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATA4750.024.547344
GATATAC4300.022.0026471
TACACCG703.21545E-521.9999395
TAGTACT1205.984475E-1021.9999394
TAGCGGC3200.019.93744530
CGCAATA3350.019.70143736
TCGTATA808.988839E-519.24994744
ATCACGG1901.8189894E-1218.52626235
ATACACT5750.018.3651664
AGGATCG600.00741225318.3332825
AATCCCG3050.018.03273619
ATCCCGT3100.017.74188620
CGAATGT3100.017.74188633
GTACACG750.001289837617.6021181
CGTTTCC3500.017.5999524
GTACGAC750.001290902717.599953
CTAGCGG3650.017.47940329
CGGTCCA4050.017.38266810
CGGTGAT1408.3724444E-817.28566643
TCTAGCG3700.017.24319628
Produced by FastQC (version 0.11.5)