##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062463_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1218386
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.214075834751878	32.0	32.0	32.0	32.0	32.0
2	31.397330566831858	32.0	32.0	32.0	32.0	32.0
3	31.477406995812494	32.0	32.0	32.0	32.0	32.0
4	31.587777600858843	32.0	32.0	32.0	32.0	32.0
5	31.521437376988903	32.0	32.0	32.0	32.0	32.0
6	35.09669021147649	36.0	36.0	36.0	36.0	36.0
7	35.10463596922486	36.0	36.0	36.0	36.0	36.0
8	35.05586735238258	36.0	36.0	36.0	36.0	36.0
9	35.15996244211605	36.0	36.0	36.0	36.0	36.0
10	35.05395744862466	36.0	36.0	36.0	36.0	36.0
11	35.16982549044391	36.0	36.0	36.0	36.0	36.0
12	35.07936893562467	36.0	36.0	36.0	36.0	36.0
13	35.123002890709515	36.0	36.0	36.0	36.0	36.0
14	35.07185325504397	36.0	36.0	36.0	36.0	36.0
15	35.04876697532637	36.0	36.0	36.0	36.0	36.0
16	35.05002437651122	36.0	36.0	36.0	36.0	36.0
17	35.0256043651191	36.0	36.0	36.0	36.0	36.0
18	35.03305684733738	36.0	36.0	36.0	36.0	36.0
19	35.00426876211644	36.0	36.0	36.0	36.0	36.0
20	34.99384431534834	36.0	36.0	36.0	36.0	36.0
21	34.95155640330733	36.0	36.0	36.0	36.0	36.0
22	34.927076476584595	36.0	36.0	36.0	32.0	36.0
23	34.856817954244384	36.0	36.0	36.0	32.0	36.0
24	34.841919555871456	36.0	36.0	36.0	32.0	36.0
25	34.811520322787686	36.0	36.0	36.0	32.0	36.0
26	34.75260877915537	36.0	36.0	36.0	32.0	36.0
27	34.73399973407442	36.0	36.0	36.0	32.0	36.0
28	34.69271150522084	36.0	36.0	36.0	32.0	36.0
29	34.66361317349346	36.0	36.0	36.0	32.0	36.0
30	34.630788600656935	36.0	36.0	36.0	32.0	36.0
31	34.62356428914974	36.0	36.0	36.0	32.0	36.0
32	34.57205023695282	36.0	36.0	36.0	32.0	36.0
33	34.51766189040255	36.0	36.0	36.0	32.0	36.0
34	34.48840597314808	36.0	36.0	36.0	32.0	36.0
35	34.406957236869104	36.0	36.0	36.0	32.0	36.0
36	34.368998823033095	36.0	36.0	36.0	32.0	36.0
37	34.309074464086095	36.0	36.0	36.0	32.0	36.0
38	34.23713831248882	36.0	36.0	36.0	32.0	36.0
39	34.11955981109435	36.0	36.0	36.0	32.0	36.0
40	34.03728375079819	36.0	36.0	36.0	32.0	36.0
41	34.02903595412291	36.0	36.0	36.0	32.0	36.0
42	33.89635878941485	36.0	36.0	36.0	32.0	36.0
43	33.925547404517125	36.0	36.0	36.0	32.0	36.0
44	33.89935209367146	36.0	36.0	36.0	32.0	36.0
45	33.709116815196495	36.0	36.0	36.0	27.0	36.0
46	33.80886024626022	36.0	36.0	36.0	32.0	36.0
47	33.71868274914518	36.0	36.0	36.0	27.0	36.0
48	33.70742933684399	36.0	36.0	36.0	27.0	36.0
49	33.72673930921727	36.0	36.0	36.0	27.0	36.0
50	33.1191100357358	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	6.0
21	35.0
22	124.0
23	384.0
24	1135.0
25	2757.0
26	5787.0
27	11112.0
28	18263.0
29	27169.0
30	37005.0
31	50875.0
32	74014.0
33	104414.0
34	206421.0
35	678881.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.60970540334655	17.63335982032625	12.209655493971473	25.547279282355724
2	14.921712636621217	21.596165905516724	36.78734532130815	26.694776136553912
3	18.68623694781094	25.604779469097437	28.763323040961016	26.9456605421306
4	12.231847897011207	16.276382260123032	35.63971979300467	35.852050049861084
5	13.492029619513026	37.5076535679169	33.75268593040301	15.247630882167062
6	33.731156346003424	34.93266884496721	17.43494030192526	13.9012345071041
7	28.790489049028018	31.165107909240515	21.551397957131776	18.493005084599694
8	28.444269714195663	32.518101816665656	20.511397865701017	18.526230603437664
9	27.30251332500538	14.558932883339107	18.612574340151646	39.52597945150387
10	15.626985208300162	27.51870096997175	32.07776517458342	24.776548647144665
11	37.00379026022952	21.33314072880023	21.556633119553243	20.106435891417007
12	23.541225851249113	24.247816373464566	29.528737198227816	22.6822205770585
13	30.482622091849382	19.749159954234536	25.3573990508755	24.410818903040578
14	24.084649692297845	19.476257934677516	25.301833737419834	31.13725863560481
15	25.522863854312178	26.635401260355913	24.424771788251014	23.41696309708089
16	25.77040445310435	25.474274983461726	25.28829123118618	23.467029332247744
17	23.672382972227194	25.632352965316414	27.027559410564468	23.66770465189193
18	25.225257020353155	23.865835621880095	28.385339293130418	22.52356806463633
19	24.546982647535344	27.14016740179221	25.648686048592158	22.664163902080293
20	24.5806337236311	23.538599425797734	28.27396243883301	23.606804411738153
21	25.69899851114507	25.399011479120738	24.225984211900006	24.676005797834183
22	24.153347133010392	25.66953330061245	25.590904688661887	24.586214877715275
23	22.982617988059616	24.792060972466853	25.64802944222931	26.577291597244223
24	23.14537429024956	26.78502543528898	25.699244738531142	24.370355535930322
25	25.021216593099393	24.342778068690873	25.153030320440322	25.48297501776941
26	22.858683537072817	25.31439133410922	26.59518412063172	25.231741008186244
27	25.39334824924121	25.589919779117622	25.935212650178187	23.081519321462984
28	23.942740642128193	24.187162360696856	26.473055337142743	25.397041660032205
29	23.118863808349737	25.729694858608028	27.325084168728136	23.826357164314103
30	24.761857079776032	24.88103113463221	26.895335304246764	23.46177648134499
31	23.308376819825572	25.279509121083137	25.813904624642763	25.598209434448528
32	23.3782233216731	25.86085197958611	26.02516772188781	24.735756976852986
33	23.23048689003321	24.85846029090945	26.489798799395263	25.421254019662076
34	23.30952588096055	26.044127230614926	27.353564469716495	23.29278241870803
35	25.45966549188845	25.057329943055812	26.31588018903697	23.167124376018766
36	23.669346167799038	26.317603780739436	26.04568667072668	23.96736338073484
37	25.186271017559296	25.74693077563268	26.10002084725202	22.966777359556005
38	24.007908823640456	25.478132545843437	25.437669178733174	25.076289451782934
39	24.026868332367574	25.108955618334424	26.441702383316944	24.42247366598106
40	25.40533131536311	25.318741351263064	25.30199788901054	23.973929444363282
41	22.801312556119324	25.632188813725698	27.04922742053832	24.517271209616656
42	25.984622278982194	25.886213400350954	25.86044160060933	22.268722720057518
43	24.53762600686482	24.011766386022163	25.702281542959295	25.748326064153726
44	23.862880893247297	25.10476975277129	27.05776330325529	23.974586050726124
45	24.459407773891034	25.415098335010416	25.899345527607835	24.226148363490715
46	23.30109119859486	24.87719399040533	27.183525732834855	24.63818907816495
47	24.53540996039022	24.39661979044408	27.443519541426113	23.624450707739584
48	24.107384687611315	27.574676662404197	24.75126930217517	23.566669347809317
49	22.92508285551541	25.420761564889943	27.757377382865528	23.896778196729116
50	23.07831836544412	27.681785575343117	24.22787195519318	25.01202410401958
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	27.0
1	31.5
2	36.0
3	167.5
4	299.0
5	323.5
6	348.0
7	340.0
8	332.0
9	425.0
10	518.0
11	812.0
12	1106.0
13	1896.0
14	2686.0
15	3594.0
16	4502.0
17	5271.5
18	6041.0
19	6514.5
20	6988.0
21	7565.0
22	8142.0
23	8838.0
24	9534.0
25	9678.5
26	9823.0
27	11757.5
28	13692.0
29	16065.0
30	18438.0
31	20364.5
32	22291.0
33	25385.5
34	28480.0
35	32374.0
36	36268.0
37	41127.5
38	45987.0
39	49061.0
40	52135.0
41	56273.5
42	60412.0
43	64653.0
44	68894.0
45	81549.0
46	94204.0
47	104832.0
48	115460.0
49	110709.0
50	105958.0
51	93119.5
52	80281.0
53	76208.5
54	72136.0
55	72634.0
56	73132.0
57	71457.5
58	69783.0
59	63407.0
60	57031.0
61	49643.5
62	42256.0
63	36822.5
64	31389.0
65	26885.0
66	22381.0
67	19748.5
68	17116.0
69	16184.0
70	15252.0
71	11887.5
72	8523.0
73	7620.5
74	6718.0
75	4788.5
76	2859.0
77	2673.5
78	2488.0
79	2100.0
80	1712.0
81	1385.0
82	1058.0
83	907.0
84	756.0
85	659.5
86	563.0
87	408.5
88	254.0
89	151.0
90	48.0
91	37.0
92	26.0
93	19.5
94	13.0
95	7.5
96	2.0
97	3.5
98	5.0
99	4.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01460949157327809
2	0.004432092949196724
3	6.566063628439592E-4
4	8.20757953554949E-5
5	0.0
6	4.103789767774745E-4
7	8.20757953554949E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	8.20757953554949E-5
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1218386.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.62751732297413
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.57806106896061	42.67573848504379
2	12.600233334957686	13.514384628879188
3	3.576681077084872	5.754255792603683
4	1.4073093200562554	3.0188201976039912
5	0.7382682165605331	1.97957457863006
6	0.43019337681087483	1.3842121660292357
7	0.28314690803294434	1.0629126000838303
8	0.1901697631077079	0.8158665812291594
9	0.14969795142899822	0.722513653512511
>10	0.854852713928516	8.497791547491174
>50	0.08963703680988443	3.363484518735115
>100	0.08866499280994228	9.977163736125846
>500	0.009235933730292738	3.3747278862303354
>1k	0.0035404412632788827	2.684031762747807
>5k	3.0786445767642457E-4	1.174521865054309
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	7541	0.618933572775787	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	6687	0.5488408435421943	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	2776	0.22784240790685384	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	2705	0.22201502643661367	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	1765	0.1448637788024485	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	1712	0.14051376164860727	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	1708	0.14018545846718528	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	1696	0.13920054892291933	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1366	0.11211553645560603	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1341	0.11006364157171865	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1329	0.10907873202745272	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1311	0.1076013677110538	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	1275	0.104646639078256	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1254	0.10292304737579058	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1250	0.10259474419436862	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1247	0.10234851680830212	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	1244	0.10210228942223565	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	8.207579535549489E-5	0.0
10	0.0	0.0	0.0	8.207579535549489E-5	0.0
11	0.0	0.0	0.0	8.207579535549489E-5	0.0
12	0.0	0.0	0.0	8.207579535549489E-5	0.0
13	0.0	0.0	0.0	8.207579535549489E-5	0.0
14	0.0	0.0	0.0	8.207579535549489E-5	0.0
15	0.0	0.0	0.0	8.207579535549489E-5	0.0
16	0.0	0.0	0.0	3.2830318142197955E-4	0.0
17	0.0	0.0	0.0	4.103789767774745E-4	0.0
18	0.0	0.0	0.0	4.924547721329693E-4	0.0
19	0.0	0.0	0.0	4.924547721329693E-4	0.0
20	0.0	0.0	0.0	6.566063628439591E-4	0.0
21	0.0	0.0	0.0	9.028337489104438E-4	0.0
22	1.6415159071098977E-4	0.0	0.0	0.001477364316398908	0.0
23	1.6415159071098977E-4	0.0	0.0	0.0033651076095752904	0.0
24	1.6415159071098977E-4	0.0	0.0	0.006073608856306622	0.0
25	1.6415159071098977E-4	0.0	0.0	0.007961352149483004	0.0
26	1.6415159071098977E-4	0.0	0.0	0.01108023237299181	0.0
27	1.6415159071098977E-4	0.0	0.0	0.015019870550055565	8.207579535549489E-5
28	1.6415159071098977E-4	0.0	0.0	0.024130283834515497	8.207579535549489E-5
29	1.6415159071098977E-4	0.0	0.0	0.0402171397241925	8.207579535549489E-5
30	1.6415159071098977E-4	0.0	0.0	0.06886159230326021	8.207579535549489E-5
31	1.6415159071098977E-4	0.0	0.0	0.12335992041930882	8.207579535549489E-5
32	1.6415159071098977E-4	0.0	0.0	0.19542246874143335	8.207579535549489E-5
33	1.6415159071098977E-4	0.0	0.0	0.25295760128563527	8.207579535549489E-5
34	1.6415159071098977E-4	0.0	0.0	0.33158621323619936	8.207579535549489E-5
35	1.6415159071098977E-4	0.0	0.0	0.41226672007065085	8.207579535549489E-5
36	1.6415159071098977E-4	0.0	0.0	0.5418644009369773	8.207579535549489E-5
37	1.6415159071098977E-4	0.0	0.0	0.7349887474084568	8.207579535549489E-5
38	1.6415159071098977E-4	0.0	0.0	0.9476471331745441	8.207579535549489E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGTATA	475	0.0	24.5473	44
GATATAC	430	0.0	22.002647	1
TACACCG	70	3.21545E-5	21.999939	5
TAGTACT	120	5.984475E-10	21.999939	4
TAGCGGC	320	0.0	19.937445	30
CGCAATA	335	0.0	19.701437	36
TCGTATA	80	8.988839E-5	19.249947	44
ATCACGG	190	1.8189894E-12	18.526262	35
ATACACT	575	0.0	18.365166	4
AGGATCG	60	0.007412253	18.333282	5
AATCCCG	305	0.0	18.032736	19
ATCCCGT	310	0.0	17.741886	20
CGAATGT	310	0.0	17.741886	33
GTACACG	75	0.0012898376	17.602118	1
CGTTTCC	350	0.0	17.59995	24
GTACGAC	75	0.0012909027	17.59995	3
CTAGCGG	365	0.0	17.479403	29
CGGTCCA	405	0.0	17.382668	10
CGGTGAT	140	8.3724444E-8	17.285666	43
TCTAGCG	370	0.0	17.243196	28
>>END_MODULE