FastQCFastQC Report
Thu 2 Feb 2017
SRR4062462_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062462_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences906578
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG63520.7006567554032858No Hit
TCCATGTACTCTGCGTTGATACCAC54700.6033678293539No Hit
GTATCAACGCAGAGTACTTTTTTTT31050.34249672945957216No Hit
GTACTTTTTTTTTTTTTTTTTTTTT28960.31944300435263157No Hit
GAGTACATGGAAGCAGTGGTATCAA26940.2971614135794162No Hit
TATCAACGCAGAGTACTTTTTTTTT22780.2512745731751708No Hit
CATGTACTCTGCGTTGATACCACTG22110.24388414455237167No Hit
GAGTACTTTTTTTTTTTTTTTTTTT18950.20902779462991602No Hit
GCTTCCATGTACTCTGCGTTGATAC16710.18431949595070696No Hit
GCGTTGATACCACTGCTTCCATGTA15860.17494357904118565No Hit
CATGGAAGCAGTGGTATCAACGCAG14450.15939058746186208No Hit
ACGCAGAGTACTTTTTTTTTTTTTT14360.15839784331850099No Hit
GTATCAACGCAGAGTACATGGAAGC13110.1446097302162638No Hit
CCCATGTACTCTGCGTTGATACCAC12420.13699869178382887No Hit
GGTATCAACGCAGAGTACTTTTTTT11050.12188691982377689No Hit
ACGCAGAGTACATGGAAGCAGTGGT9710.10710606257817859No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAG801.2853343E-714.25095410
CGACCCG801.2879354E-714.2485925
AATTCCG707.261946E-613.57008655
GCAAGAC1400.013.5663393
TTTGCGC500.001496077913.30309617
ACCGTCC500.001497948213.300898
GTCCGTT500.001498572113.30015511
AATCGTA500.001498572113.30015513
TCGCGTA655.444164E-513.1547269
GCGTAAC801.99651E-613.06265311
CGCAAGA1403.6379788E-1212.887312
GCACCGT604.0867855E-412.6675146
CGCGTAA751.4759531E-512.66751410
CAAGACG1352.7284841E-1112.6647154
GTCTAAC604.1135345E-412.6577221
AGGCCCG1001.438566E-712.35082610
TATACTG1251.4078978E-912.1587985
GCGGTCG550.003065207512.0917179
CCGTTGA550.003066476412.0910513
GACTGCT802.8613014E-511.8757957
Produced by FastQC (version 0.11.5)