##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062462_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	906578
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.026204033188538	32.0	32.0	32.0	32.0	32.0
2	30.763152205325962	32.0	32.0	32.0	32.0	32.0
3	30.763208460827418	32.0	32.0	32.0	32.0	32.0
4	30.784129992124228	32.0	32.0	32.0	32.0	32.0
5	30.650581637763104	32.0	32.0	32.0	32.0	32.0
6	34.25339904564196	36.0	36.0	36.0	32.0	36.0
7	34.10500585719045	36.0	36.0	36.0	32.0	36.0
8	34.0933146403288	36.0	36.0	36.0	32.0	36.0
9	34.21899935802546	36.0	36.0	36.0	32.0	36.0
10	33.93774060257363	36.0	36.0	36.0	32.0	36.0
11	34.25120066888894	36.0	36.0	36.0	32.0	36.0
12	34.049302983306454	36.0	36.0	36.0	32.0	36.0
13	34.14965507656263	36.0	36.0	36.0	32.0	36.0
14	34.05546461528959	36.0	36.0	36.0	32.0	36.0
15	34.031096055717214	36.0	36.0	36.0	32.0	36.0
16	34.02303607632217	36.0	36.0	36.0	32.0	36.0
17	33.953118209354294	36.0	36.0	36.0	32.0	36.0
18	33.969850360366124	36.0	36.0	36.0	32.0	36.0
19	33.955718095960854	36.0	36.0	36.0	32.0	36.0
20	33.92805583193062	36.0	36.0	36.0	32.0	36.0
21	33.88991460194269	36.0	36.0	36.0	32.0	36.0
22	33.8433879930905	36.0	36.0	36.0	32.0	36.0
23	33.80503497768532	36.0	36.0	36.0	32.0	36.0
24	33.793618420036665	36.0	36.0	36.0	32.0	36.0
25	33.32319447416549	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	4.0
4	34.0
5	92.0
6	168.0
7	39.0
8	136.0
9	179.0
10	114.0
11	38.0
12	65.0
13	46.0
14	238.0
15	307.0
16	420.0
17	512.0
18	773.0
19	985.0
20	1554.0
21	2319.0
22	3520.0
23	4720.0
24	6417.0
25	8702.0
26	11681.0
27	14602.0
28	19776.0
29	25777.0
30	32705.0
31	44810.0
32	62641.0
33	90466.0
34	199819.0
35	372918.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.636545154689216	17.51956712392236	11.619369108489291	25.224518612899132
2	15.779342691931793	20.817917678749893	37.59467252627004	25.80806710304827
3	19.2408244129301	25.446204011754638	28.225685466434868	27.0872861088804
4	12.35798996083623	16.113188813503225	35.918031882619005	35.61078934304154
5	13.816378956474914	37.45492344157593	33.825515649345206	14.903181952603958
6	33.238978094134524	35.21789990619655	17.796501682944324	13.746620316724604
7	28.913062675232354	31.13881714020796	21.68289290681502	18.265227277744664
8	28.03457454736556	32.8834682303012	20.409316888820683	18.672640333512557
9	27.47015170101903	14.846802839466777	18.974497136352042	38.70854832316215
10	15.708189392399918	27.573818506312787	32.26820723960312	24.449784861684176
11	36.206970813762275	21.84301698250946	22.230996144505397	19.71901605922287
12	24.055632626732997	24.39118678813477	29.586753673644306	21.966426911487925
13	29.993815229996134	20.298746479651005	25.33591032083494	24.37152796951792
14	23.622678601997933	20.047822317449622	25.752594057000227	30.576905023552214
15	24.941580270254747	27.19167855675659	24.170638619306562	23.6961025536821
16	24.939013818381	26.066416132819015	25.309734708729554	23.684835340070435
17	23.207653236467735	26.01786741797146	26.75319483084046	24.021284514720353
18	24.17903095222579	24.700810614085178	28.294617738962163	22.825540694726868
19	24.496970834967115	26.547745936721658	26.119189498158203	22.83609373015303
20	24.619387729181874	24.474376800902096	27.507347183552028	23.398888286364002
21	25.697841504132434	25.266208599744424	24.721807733386054	24.31414216273709
22	24.57920529567208	25.858933795261112	25.50431289715331	24.057548011913493
23	23.325810873654714	25.504562631380693	25.897365371721076	25.272261123243517
24	23.510718672404497	26.491379995935212	25.987257769491105	24.010643562169186
25	24.401900663597566	25.25779572505324	25.7795725053238	24.560731106025397
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	359.0
1	359.0
2	706.0
3	1053.0
4	1053.0
5	1053.0
6	2643.0
7	4233.0
8	4233.0
9	4233.0
10	4074.0
11	3915.0
12	3915.0
13	3915.0
14	3683.0
15	3451.0
16	3451.0
17	3451.0
18	5531.5
19	7612.0
20	7612.0
21	7612.0
22	11908.5
23	16205.0
24	16205.0
25	16205.0
26	23445.5
27	30686.0
28	30686.0
29	30686.0
30	37365.5
31	44045.0
32	44045.0
33	44045.0
34	52108.0
35	60171.0
36	60171.0
37	60171.0
38	67180.5
39	74190.0
40	74190.0
41	74190.0
42	85389.5
43	96589.0
44	96589.0
45	96589.0
46	117968.5
47	139348.0
48	139348.0
49	139348.0
50	129945.0
51	120542.0
52	120542.0
53	120542.0
54	107156.0
55	93770.0
56	93770.0
57	93770.0
58	84820.0
59	75870.0
60	75870.0
61	75870.0
62	66733.0
63	57596.0
64	57596.0
65	57596.0
66	47273.0
67	36950.0
68	36950.0
69	36950.0
70	27934.0
71	18918.0
72	18918.0
73	18918.0
74	14547.0
75	10176.0
76	10176.0
77	10176.0
78	8272.0
79	6368.0
80	6368.0
81	6368.0
82	4370.0
83	2372.0
84	2372.0
85	2372.0
86	1821.0
87	1270.0
88	1270.0
89	1270.0
90	849.0
91	428.0
92	428.0
93	428.0
94	256.5
95	85.0
96	85.0
97	85.0
98	230.5
99	376.0
100	376.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009155307099885504
2	0.003529756954172724
3	0.004632806002351701
4	0.014119027816690896
5	0.03772427744772099
6	0.04721049926206019
7	0.07037452927381868
8	0.09100154647476555
9	0.10092898790837633
10	0.12287966396713795
11	0.12321057868159165
12	0.13346893482965613
13	0.12442393263458852
14	0.12607850620685698
15	0.11714380891660729
16	0.11526862553470302
17	0.12475484734904223
18	0.13402045935374562
19	0.13379984954410984
20	0.12453423753940643
21	0.13004948278030132
22	0.15288259807760612
23	0.14218302231027005
24	0.13699869178382887
25	0.13346893482965613
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	906578.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.6001597053167
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.69227012075926	48.45780235471681
2	11.616631453804976	13.614729168615542
3	2.711435948229759	4.766717387910021
4	1.013367468806842	2.375339820490139
5	0.4641344946424388	1.3599177755396685
6	0.2832869974710291	0.9960397976545172
7	0.1736275948849747	0.7122223352656682
8	0.13058223190936122	0.6121711715652216
9	0.09492063755787153	0.5006128068199579
>10	0.6394171677027901	7.414804905053746
>50	0.08997636526194747	3.7318136063561513
>100	0.08223881061821826	9.304828430670167
>500	0.005281391484050983	2.0967973545246807
>1k	0.0024520746175950995	2.7494904688350785
>5k	3.772422488607845E-4	1.306712615982645
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	6352	0.7006567554032858	No Hit
TCCATGTACTCTGCGTTGATACCAC	5470	0.6033678293539	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3105	0.34249672945957216	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2896	0.31944300435263157	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2694	0.2971614135794162	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2278	0.2512745731751708	No Hit
CATGTACTCTGCGTTGATACCACTG	2211	0.24388414455237167	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1895	0.20902779462991602	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1671	0.18431949595070696	No Hit
GCGTTGATACCACTGCTTCCATGTA	1586	0.17494357904118565	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1445	0.15939058746186208	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1436	0.15839784331850099	No Hit
GTATCAACGCAGAGTACATGGAAGC	1311	0.1446097302162638	No Hit
CCCATGTACTCTGCGTTGATACCAC	1242	0.13699869178382887	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1105	0.12188691982377689	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	971	0.10710606257817859	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.1030490481789763E-4	0.0
11	0.0	0.0	0.0	1.1030490481789763E-4	0.0
12	0.0	0.0	0.0	2.2060980963579526E-4	0.0
13	0.0	0.0	0.0	2.2060980963579526E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTAG	80	1.2853343E-7	14.250954	10
CGACCCG	80	1.2879354E-7	14.248592	5
AATTCCG	70	7.261946E-6	13.5700865	5
GCAAGAC	140	0.0	13.566339	3
TTTGCGC	50	0.0014960779	13.303096	17
ACCGTCC	50	0.0014979482	13.30089	8
GTCCGTT	50	0.0014985721	13.300155	11
AATCGTA	50	0.0014985721	13.300155	13
TCGCGTA	65	5.444164E-5	13.154726	9
GCGTAAC	80	1.99651E-6	13.062653	11
CGCAAGA	140	3.6379788E-12	12.88731	2
GCACCGT	60	4.0867855E-4	12.667514	6
CGCGTAA	75	1.4759531E-5	12.667514	10
CAAGACG	135	2.7284841E-11	12.664715	4
GTCTAAC	60	4.1135345E-4	12.657722	1
AGGCCCG	100	1.438566E-7	12.350826	10
TATACTG	125	1.4078978E-9	12.158798	5
GCGGTCG	55	0.0030652075	12.091717	9
CCGTTGA	55	0.0030664764	12.09105	13
GACTGCT	80	2.8613014E-5	11.875795	7
>>END_MODULE