FastQCFastQC Report
Thu 2 Feb 2017
SRR4062462_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062462_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences906578
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA36270.40007588977451475No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC29420.32451702997425486No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC12870.14196241250063424No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA12710.1401975340235479No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACCGT551.5932573E-423.999856
ACGAACC551.5932573E-423.9998526
ACCGTCG1750.022.628438
CGTCGTA1600.021.99986310
TAGGACG2900.021.9998634
TACCGTC1700.020.7057517
ATACCGT1950.020.3075666
ATCGTTT2200.019.99987429
CCGTCGT2000.019.7998759
TGTAGGA4600.019.6107352
TTAGGAC2600.019.4624923
CCTAGTA808.978236E-519.2520032
CGCATCG2300.019.13031613
ATGGTCG2550.018.98027436
AGGACGT3500.018.8570235
GCATTAT1203.1563468E-718.3352411
GCACCGC600.007410975618.333226
GGACGTG3650.018.0820776
CGAGCCG2800.018.07131415
GTCGTAG1951.8189894E-1218.05116811
Produced by FastQC (version 0.11.5)