##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062462_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	906578
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.232289996006962	32.0	32.0	32.0	32.0	32.0
2	31.422706044046954	32.0	32.0	32.0	32.0	32.0
3	31.503389669725053	32.0	32.0	32.0	32.0	32.0
4	31.604902170579916	32.0	32.0	32.0	32.0	32.0
5	31.546836565634727	32.0	32.0	32.0	32.0	32.0
6	35.130647335364415	36.0	36.0	36.0	36.0	36.0
7	35.14084722991293	36.0	36.0	36.0	36.0	36.0
8	35.09738158216943	36.0	36.0	36.0	36.0	36.0
9	35.20871232260214	36.0	36.0	36.0	36.0	36.0
10	35.100577115262006	36.0	36.0	36.0	36.0	36.0
11	35.21352382255029	36.0	36.0	36.0	36.0	36.0
12	35.133459007388225	36.0	36.0	36.0	36.0	36.0
13	35.16080579939067	36.0	36.0	36.0	36.0	36.0
14	35.12253440961506	36.0	36.0	36.0	36.0	36.0
15	35.09698448451209	36.0	36.0	36.0	36.0	36.0
16	35.09860155441672	36.0	36.0	36.0	36.0	36.0
17	35.089652517488844	36.0	36.0	36.0	36.0	36.0
18	35.08740009133246	36.0	36.0	36.0	36.0	36.0
19	35.06231013768258	36.0	36.0	36.0	36.0	36.0
20	35.04123197342093	36.0	36.0	36.0	36.0	36.0
21	35.017495460953164	36.0	36.0	36.0	36.0	36.0
22	35.005910136800146	36.0	36.0	36.0	36.0	36.0
23	34.950235942191405	36.0	36.0	36.0	32.0	36.0
24	34.94453317861232	36.0	36.0	36.0	32.0	36.0
25	34.91788461665737	36.0	36.0	36.0	32.0	36.0
26	34.863387375383034	36.0	36.0	36.0	32.0	36.0
27	34.843433218101474	36.0	36.0	36.0	32.0	36.0
28	34.828223274776136	36.0	36.0	36.0	32.0	36.0
29	34.78897017134764	36.0	36.0	36.0	32.0	36.0
30	34.76340921575419	36.0	36.0	36.0	32.0	36.0
31	34.74605604812824	36.0	36.0	36.0	32.0	36.0
32	34.7027657851834	36.0	36.0	36.0	32.0	36.0
33	34.667517852848846	36.0	36.0	36.0	32.0	36.0
34	34.63656188436075	36.0	36.0	36.0	32.0	36.0
35	34.577550966381274	36.0	36.0	36.0	32.0	36.0
36	34.540766486722596	36.0	36.0	36.0	32.0	36.0
37	34.51351896913448	36.0	36.0	36.0	32.0	36.0
38	34.456250868651125	36.0	36.0	36.0	32.0	36.0
39	34.38501375502163	36.0	36.0	36.0	32.0	36.0
40	34.336656084749464	36.0	36.0	36.0	32.0	36.0
41	34.321237665154015	36.0	36.0	36.0	32.0	36.0
42	34.2276318198765	36.0	36.0	36.0	32.0	36.0
43	34.22999786008484	36.0	36.0	36.0	32.0	36.0
44	34.1901689650532	36.0	36.0	36.0	32.0	36.0
45	34.08530981338616	36.0	36.0	36.0	32.0	36.0
46	34.1042789478677	36.0	36.0	36.0	32.0	36.0
47	34.04285235247271	36.0	36.0	36.0	32.0	36.0
48	34.001626997346065	36.0	36.0	36.0	32.0	36.0
49	34.001626997346065	36.0	36.0	36.0	32.0	36.0
50	33.43917236023817	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	6.0
21	34.0
22	95.0
23	229.0
24	739.0
25	1759.0
26	3624.0
27	6664.0
28	11427.0
29	17219.0
30	23807.0
31	33477.0
32	48857.0
33	72989.0
34	150738.0
35	534912.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.24492894006959	18.03086434735409	12.08140173738827	25.64280497518805
2	15.351760152050486	21.341119706665225	37.27432074224994	26.03279939903435
3	18.50103301340987	25.763012494333044	28.697145617938364	27.038808874318725
4	12.209442772097681	16.543216957853552	36.1294186814799	35.11792158856887
5	13.568385731839951	37.61297979875973	33.78319350348233	15.03544096591799
6	33.233543502336275	35.55999964702197	17.48465648619194	13.721800364449818
7	28.8118617482445	31.13389029956606	21.793932789015397	18.260315163174045
8	28.007187467597934	32.95877464487336	20.349710670234664	18.684327217294044
9	27.514345152871567	14.619260560040063	18.79573517116012	39.070659115928244
10	15.58575213605448	27.58758760967065	32.38485822510584	24.44180202916903
11	36.52504252254081	21.625276589548832	22.255007291154207	19.594673596756156
12	24.221633439152505	24.387862930713077	29.49685520716364	21.893648422970777
13	30.221668736722044	20.22021271197845	25.25563161691548	24.302486934384024
14	23.735188808905576	19.854331342697485	25.7278469144409	30.68263293395604
15	24.981634233347823	27.296823880570674	24.140890248825805	23.580651637255702
16	25.024984060941254	25.944926967122523	25.282104794071774	23.747984177864453
17	23.32000114717101	26.001292773484465	26.779714486784368	23.898991592560154
18	24.418858608966907	24.43595586921368	28.399542014035195	22.745643507784216
19	24.615311644447583	26.475603864201425	26.191348124485703	22.71773636686529
20	24.871219023625105	24.22825173344158	27.550414856747018	23.350114386186295
21	25.746929662974395	25.086313588020005	24.956595019954158	24.210161729051443
22	24.6271142692631	25.705565323667685	25.595811943373874	24.071508463695345
23	23.415414889838495	25.14179695514341	26.09339736900741	25.34939078601069
24	23.62212628146723	26.228962097028607	26.154065066657257	23.99484655484691
25	24.65634506903984	24.888316283871877	25.925072084255298	24.530266562832985
26	23.168221598141585	25.50117033504012	26.899395308511785	24.43121275830651
27	24.96001447200351	25.80958284891096	25.99279929581349	23.23760338327204
28	23.81008583927693	24.739845881986987	26.59263736821321	24.85743091052287
29	23.366880731718616	25.87510396237279	27.103459382424898	23.654555923483695
30	24.475334720233672	25.122603901705094	26.93447226824388	23.467589109817357
31	23.472552830534163	25.402116530513645	26.21936557030945	24.905965068642743
32	23.437255260992433	25.927829706875745	26.293380161442258	24.34153487068956
33	23.151675862418898	25.340235478910806	26.680770987162717	24.827317671507583
34	23.392912689255642	26.051150590462157	27.305096748432018	23.250839971850187
35	24.91346580217036	25.426824829192856	26.31301443449984	23.34669493413694
36	23.529028941800924	26.15075591951272	26.397507991590352	23.922707147096002
37	24.70079794568145	25.844439198833413	25.999638199912194	23.455124655572934
38	23.755705521201705	25.882163476281132	25.836607550591346	24.525523451925814
39	23.85994365625462	25.6446770162082	26.242198685606756	24.253180641930424
40	24.88611018577552	25.529408390673495	25.836828160400984	23.74765326315
41	23.27907802748357	25.680195195559563	26.897078905510615	24.14364787144625
42	24.93078367222677	26.021478571066144	26.19101720977125	22.85672054693584
43	23.923589586334547	24.955712580715613	26.132886524932218	24.987811308017623
44	23.563333767199293	25.572316998647658	26.86773780082905	23.996611433323995
45	23.91862586561774	25.786087904184747	26.2671275940956	24.028158636101914
46	23.291483560120717	25.321539506891867	26.89713824323609	24.489838689751327
47	24.14905281178233	24.925268426985873	27.119343288718678	23.806335472513123
48	23.74544716505364	27.193909404375578	25.37354756016581	23.687095870404974
49	23.013243206872435	25.780903573658303	27.05867559106883	24.147177628400424
50	22.91242452386888	27.40404024805367	25.088299076306726	24.595236151770724
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	80.0
1	68.5
2	57.0
3	199.0
4	341.0
5	369.0
6	397.0
7	343.5
8	290.0
9	409.0
10	528.0
11	730.0
12	932.0
13	1603.0
14	2274.0
15	3069.5
16	3865.0
17	4337.0
18	4809.0
19	5224.5
20	5640.0
21	6191.5
22	6743.0
23	7027.0
24	7311.0
25	7771.0
26	8231.0
27	9739.0
28	11247.0
29	12786.5
30	14326.0
31	15974.5
32	17623.0
33	20492.0
34	23361.0
35	26532.0
36	29703.0
37	33600.0
38	37497.0
39	39347.5
40	41198.0
41	44297.5
42	47397.0
43	49367.5
44	51338.0
45	58555.5
46	65773.0
47	70730.5
48	75688.0
49	73676.0
50	71664.0
51	65833.5
52	60003.0
53	57886.5
54	55770.0
55	55359.5
56	54949.0
57	53545.5
58	52142.0
59	47388.0
60	42634.0
61	37717.5
62	32801.0
63	28402.0
64	24003.0
65	20606.5
66	17210.0
67	14815.0
68	12420.0
69	11062.5
70	9705.0
71	7648.0
72	5591.0
73	4967.0
74	4343.0
75	3232.0
76	2121.0
77	1866.5
78	1612.0
79	1396.0
80	1180.0
81	960.0
82	740.0
83	620.5
84	501.0
85	419.5
86	338.0
87	237.5
88	137.0
89	85.0
90	33.0
91	22.5
92	12.0
93	10.0
94	8.0
95	7.0
96	6.0
97	5.5
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01764878477086362
2	0.004081281478262213
3	7.721343337252834E-4
4	1.1030490481789763E-4
5	0.0
6	6.618294289073858E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	2.2060980963579526E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	906578.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.023668469402374
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.19592359888432	52.43312306901814
2	11.37295753472676	14.335310103704218
3	2.6462832195732995	5.00335428919591
4	0.9562056931737707	2.410543623805552
5	0.44914731584616713	1.4153455763905394
6	0.25155697756522805	0.9512426133141492
7	0.17537840633189627	0.7737093376147497
8	0.12647932736654863	0.6376952956945897
9	0.08511288419500716	0.48277135763826906
>10	0.5877734304155768	7.237007933164026
>50	0.08170017160786112	3.6457673480543638
>100	0.06707987384556978	8.07786055058851
>500	0.003697315833098862	1.58352651926792
>1k	7.042506348759736E-4	1.0127423825491069
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	3627	0.40007588977451475	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2942	0.32451702997425486	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	1287	0.14196241250063424	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	1271	0.1401975340235479	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.2060980963579526E-4
2	0.0	0.0	0.0	0.0	2.2060980963579526E-4
3	0.0	0.0	0.0	0.0	2.2060980963579526E-4
4	0.0	0.0	0.0	0.0	2.2060980963579526E-4
5	0.0	0.0	0.0	0.0	2.2060980963579526E-4
6	0.0	0.0	0.0	0.0	3.309147144536929E-4
7	0.0	0.0	0.0	0.0	3.309147144536929E-4
8	0.0	0.0	0.0	0.0	3.309147144536929E-4
9	0.0	0.0	0.0	0.0	3.309147144536929E-4
10	0.0	0.0	0.0	0.0	3.309147144536929E-4
11	0.0	0.0	0.0	0.0	3.309147144536929E-4
12	0.0	0.0	0.0	0.0	4.412196192715905E-4
13	0.0	0.0	0.0	0.0	4.412196192715905E-4
14	0.0	0.0	0.0	0.0	4.412196192715905E-4
15	0.0	0.0	0.0	0.0	5.515245240894881E-4
16	0.0	0.0	0.0	2.2060980963579526E-4	5.515245240894881E-4
17	0.0	0.0	0.0	2.2060980963579526E-4	5.515245240894881E-4
18	0.0	0.0	0.0	2.2060980963579526E-4	5.515245240894881E-4
19	0.0	0.0	0.0	4.412196192715905E-4	5.515245240894881E-4
20	0.0	0.0	0.0	6.618294289073858E-4	6.618294289073858E-4
21	0.0	0.0	0.0	0.001213353952996874	6.618294289073858E-4
22	0.0	0.0	0.0	0.0016545735722684645	6.618294289073858E-4
23	0.0	0.0	0.0	0.004963720716805393	6.618294289073858E-4
24	0.0	0.0	0.0	0.0077213433372528345	6.618294289073858E-4
25	0.0	0.0	0.0	0.010809880672153969	6.618294289073858E-4
26	0.0	0.0	0.0	0.013346893482965614	6.618294289073858E-4
27	0.0	0.0	0.0	0.01820030929495311	6.618294289073858E-4
28	0.0	0.0	0.0	0.028127750728563897	6.618294289073858E-4
29	0.0	0.0	0.0	0.04368074230788746	6.618294289073858E-4
30	0.0	0.0	0.0	0.07026422436900079	6.618294289073858E-4
31	0.0	0.0	0.0	0.13104222692366238	6.618294289073858E-4
32	0.0	0.0	0.0	0.20384346410347481	6.618294289073858E-4
33	0.0	0.0	0.0	0.2749901277110188	6.618294289073858E-4
34	0.0	0.0	0.0	0.35176234146427554	6.618294289073858E-4
35	0.0	0.0	0.0	0.438572301555961	6.618294289073858E-4
36	0.0	0.0	0.0	0.5666362960495401	6.618294289073858E-4
37	0.0	0.0	0.0	0.7512867067147008	6.618294289073858E-4
38	0.0	0.0	0.0	0.9979284738875199	6.618294289073858E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACCGT	55	1.5932573E-4	23.99985	6
ACGAACC	55	1.5932573E-4	23.99985	26
ACCGTCG	175	0.0	22.62843	8
CGTCGTA	160	0.0	21.999863	10
TAGGACG	290	0.0	21.999863	4
TACCGTC	170	0.0	20.705751	7
ATACCGT	195	0.0	20.307566	6
ATCGTTT	220	0.0	19.999874	29
CCGTCGT	200	0.0	19.799875	9
TGTAGGA	460	0.0	19.610735	2
TTAGGAC	260	0.0	19.462492	3
CCTAGTA	80	8.978236E-5	19.252003	2
CGCATCG	230	0.0	19.130316	13
ATGGTCG	255	0.0	18.980274	36
AGGACGT	350	0.0	18.857023	5
GCATTAT	120	3.1563468E-7	18.335241	1
GCACCGC	60	0.0074109756	18.33322	6
GGACGTG	365	0.0	18.082077	6
CGAGCCG	280	0.0	18.071314	15
GTCGTAG	195	1.8189894E-12	18.051168	11
>>END_MODULE