FastQCFastQC Report
Thu 2 Feb 2017
SRR4062461_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062461_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences392729
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG113482.8895243284809626No Hit
TCCATGTACTCTGCGTTGATACCAC95202.4240634126840646No Hit
GAGTACATGGAAGCAGTGGTATCAA48471.23418438668904No Hit
CATGTACTCTGCGTTGATACCACTG42601.0847174514741718No Hit
GTACTTTTTTTTTTTTTTTTTTTTT37850.9637689093497043No Hit
GTATCAACGCAGAGTACTTTTTTTT36850.9383060583761321No Hit
CCCATGTACTCTGCGTTGATACCAC29360.747589304584077No Hit
TATCAACGCAGAGTACTTTTTTTTT28180.7175431404352619No Hit
GTACATGGGAAGCAGTGGTATCAAC27820.708376514084776No Hit
GCTTCCATGTACTCTGCGTTGATAC27210.692844174990897No Hit
GAGTACTTTTTTTTTTTTTTTTTTT25920.659997097234989No Hit
GCGTTGATACCACTGCTTCCATGTA25720.6549045270402746No Hit
CATGGAAGCAGTGGTATCAACGCAG25040.6375897883782455No Hit
GTACATGGGGTGGTATCAACGCAAA19570.4983079935528061No Hit
GTATCAACGCAGAGTACATGGAAGC19190.48863211018284874No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18630.47437291363764833No Hit
ACTCTGCGTTGATACCACTGCTTCC18340.4669886868553124No Hit
CAGTGGTATCAACGCAGAGTACATG16640.4237018402002399No Hit
ACGCAGAGTACATGGAAGCAGTGGT16060.40893338663556805No Hit
GGTATCAACGCAGAGTACTTTTTTT14140.3600447127663096No Hit
GTATCAACGCAGAGTACATGGGAAG12900.32847077755908016No Hit
TATCAACGCAGAGTACATGGAAGCA12590.32057729375727284No Hit
CCATAGGGTCTTCTCGTCTTATTAT11860.3019894125465652No Hit
GTATTAGAGGCACTGCCTGCCCAGT11840.30148015552709373No Hit
GTACATGGGTGGTATCAACGCAAAA11710.2981699849005294No Hit
GAGTACATGGGAAGCAGTGGTATCA10460.26634142118356424No Hit
CTCTAATACTTGTAATGCTAGAGGT9830.2502998250702138No Hit
ATACCACTGCTTCCATGTACTCTGC9430.24011468468078498No Hit
GCGTTGATACCACTGCTTCCCATGT9320.23731377107369203No Hit
GGTATCAACGCAGAGTACATGGAAG9310.2370591425639563No Hit
GTACATGGGAGTGGTATCAACGCAA9060.2306934298205633No Hit
GCAGAGTACTTTTTTTTTTTTTTTT8670.2207629179408702No Hit
TATCAACGCAGAGTACATGGGAAGC8420.21439720519747713No Hit
CCATTGGGATGTCCTGATCCAACAT8350.21261480562932708No Hit
GTACTCTGCGTTGATACCACTGCTT8120.2067583499054055No Hit
AAGCAGTGGTATCAACGCAGAGTAC7960.20268429374963398No Hit
GATATATTTTGATCAACGGACCAAG7520.19148063932126225No Hit
ATCTATAACTTTATAGATGCAACAC7520.19148063932126225No Hit
GTCAGGATACCGCGGCCGTTAAACT7240.18435104104866207No Hit
CTTCTACACCATTGGGATGTCCTGA7170.18256864148051202No Hit
CCACTGCTTCCATGTACTCTGCGTT7040.17925847085394764No Hit
ACGCAGAGTACATGGGAAGCAGTGG6960.17722144277606186No Hit
GTGGTATCAACGCAGAGTACATGGA6890.17543904320791182No Hit
GGTATCAACGCAGAGTACATGGGAA6740.171619615561876No Hit
GCTTCCCATGTACTCTGCGTTGATA6680.17009184450346168No Hit
GTATCAACGCAGAGTACATGGGGTG6660.16958258748399024No Hit
ATCCTGACCGTGCAAAGGTAGCATA6550.1667816738768973No Hit
GAAGCAGTGGTATCAACGCAGAGTA6460.16449001728927581No Hit
CTGCTTCCATGTACTCTGCGTTGAT6260.15939744709456138No Hit
GTTATATAATTTAAGCTCCATAGGG6230.15863356156535421No Hit
CCTCTAGCATTACAAGTATTAGAGG6180.1573604190166756No Hit
CTGTTAGTATGAGTAACAAGAATTC6150.15659653348746846No Hit
GTTATAGATTAACCCAATTTTAAGT6100.15532339093878986No Hit
AAAAAGTACTCTGCGTTGATACCAC6030.1535409913706398No Hit
GTATCCTGACCGTGCAAAGGTAGCA6010.15303173435116837No Hit
ACATGGAAGCAGTGGTATCAACGCA5880.149721563724604No Hit
GCCTAAAGGAAAGATCCAAAAAGAT5780.14717527862724678No Hit
GTCCTAAAGTGTGTATTTCTCATTT5560.1415734514130609No Hit
GTTGATACCACTGCTTCCATGTACT5560.1415734514130609No Hit
GTCCTACAGTGGACATTTCTAAATT5540.14106419439358947No Hit
TGGAAGCAGTGGTATCAACGCAGAG5540.14106419439358947No Hit
ATCGTAAATAGATAGAAACCGACCT5520.14055493737411803No Hit
GTTCATGCTAGTCCCTAATTAAGGA5510.1403003088643823No Hit
GGTCAGGATACCGCGGCCGTTAAAC5500.1400456803546466No Hit
ACCTATAACTTCTCTGTTAACCCAA5320.1354623671794036No Hit
GATATACACTGTTCTACAAATCCCG5200.13240682506257495No Hit
TATCAACGCAGAGTACATGGGGTGG5180.1318975680431035No Hit
GATACCACTGCTTCCATGTACTCTG5130.1306244254944249No Hit
ATAAATAATCCACCTATAACTTCTC5120.1303697969846892No Hit
TGCTAGAGGTGATGTTTTTGGTAAA5060.12884202592627486No Hit
CTATAGAACTAGTACCGCAAGGGAA5000.12731425486786052No Hit
CACTATAAATAATCCACCTATAACT4950.12604111231918194No Hit
GATTAAACCTTGTACCTTTTGCATA4920.12527722678997477No Hit
CCTATAACTTCTCTGTTAACCCAAC4770.12145779914393895No Hit
GTACATGGGAGAAATCGTAAATAGA4770.12145779914393895No Hit
GATTAAAGATAAGAGACAGTTGGAC4740.12069391361473178No Hit
ATCAACGCAGAGTACTTTTTTTTTT4680.11916614255631747No Hit
GCAGAGTACATGGAAGCAGTGGTAT4670.11891151404658175No Hit
CTTTAGGACGTGAAATATGGCGAGG4650.1184022570271103No Hit
GTGTAGAAGCTATTAATGGTTCGTT4630.11789300000763885No Hit
GACTATAGGCAATAATCACACTATA4630.11789300000763885No Hit
TTGTAGAACAGTGTATATCAATGAG4590.11687448596869596No Hit
AAATATATCTGGGTCAATAAGATAT4550.11585597192975308No Hit
CCCCATGTACTCTGCGTTGATACCA4510.1148374578908102No Hit
CTGTAGGACGTGGAATATGGCAAGA4510.1148374578908102No Hit
AGCATGAACGGCTAAACGAGGGTCC4490.11432820087133877No Hit
TACTAACAGTGTTGCATCTATAAAG4470.11381894385186732No Hit
TCCCAATGGTGTAGAAGCTATTAAT4460.1135643153421316No Hit
ATCCTAGCCCTAGCCCTACACAAAT4460.1135643153421316No Hit
GATAGAAACCGACCTGGATTGCTCC4430.11280042981292443No Hit
GTCCTGATCCAACATCGAGGTCGTA4370.1112726587545101No Hit
CCTATAGTCTGATTAACTAACAATG4330.11025414471556721No Hit
GTCCTACAGTGTGCATTTCTCATTT4300.10949025918636007No Hit
GCAGTGGTATCAACGCAGAGTACAT4280.10898100216688862No Hit
ACCCTATGGAGCTTAAATTATATAA4260.10847174514741717No Hit
GTATCAACGCAGAGTACATGGGAGA4260.10847174514741717No Hit
CTGTTAACCCAACACCGGAATGCCT4140.10541620303058852No Hit
CCAATAAAGAAAGCGTTCAAGCTCA4080.1038884319721742No Hit
GTGTATATCAATGAGTTACAATGAA4080.1038884319721742No Hit
GTTAGTATGAGTAACAAGAATTCCA3960.10083288985534554No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCAC250.00602094719.00178511
CTCGGCA607.633571E-817.41830312
TTGAACT453.5290286E-516.8883239
AAGCGTT753.252353E-916.46821411
AAAGCGT753.252353E-916.46821410
GAAAGCG753.25781E-916.4661149
CCAATTT1050.016.28724314
ACTCGGC651.9005711E-716.07843211
TCCAACG651.9005711E-716.07843218
AACGGAC1250.015.961515
GGACCAA1250.015.961518
CGGACCA1250.015.961517
GGCAAAC601.4585676E-615.83482115
GCGTTCA851.0095391E-915.64852913
GTTTAAA551.1284645E-515.538991
AGCGTTC807.6452125E-915.438951512
GAACTCG807.657945E-915.4369829
ACGGACC1300.015.34759516
GAACTTA508.680534E-515.20142811
TGAACTT508.680534E-515.20142810
Produced by FastQC (version 0.11.5)