##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062461_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	392729
Sequences flagged as poor quality	0
Sequence length	25
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.644780497493183	32.0	32.0	32.0	32.0	32.0
2	30.611210274769626	32.0	32.0	32.0	32.0	32.0
3	30.665960496933	32.0	32.0	32.0	32.0	32.0
4	30.594684884487776	32.0	32.0	32.0	32.0	32.0
5	30.609695235136698	32.0	32.0	32.0	32.0	32.0
6	34.061854357585	36.0	36.0	36.0	32.0	36.0
7	33.74653514255377	36.0	36.0	36.0	32.0	36.0
8	33.704119125401995	36.0	36.0	36.0	27.0	36.0
9	33.780293790374536	36.0	36.0	36.0	32.0	36.0
10	33.688515490325386	36.0	36.0	36.0	27.0	36.0
11	34.04503869080205	36.0	36.0	36.0	32.0	36.0
12	33.849939780357445	36.0	36.0	36.0	32.0	36.0
13	33.90746036070675	36.0	36.0	36.0	32.0	36.0
14	33.859238813533	36.0	36.0	36.0	32.0	36.0
15	33.91138927861197	36.0	36.0	36.0	32.0	36.0
16	33.82232022590642	36.0	36.0	36.0	32.0	36.0
17	33.75906795780296	36.0	36.0	36.0	32.0	36.0
18	33.853761754288584	36.0	36.0	36.0	32.0	36.0
19	33.78150072951068	36.0	36.0	36.0	32.0	36.0
20	33.64230041580836	36.0	36.0	36.0	27.0	36.0
21	33.518561145217184	36.0	36.0	36.0	27.0	36.0
22	33.480336313335656	36.0	36.0	36.0	27.0	36.0
23	33.430294172317296	36.0	36.0	36.0	27.0	36.0
24	33.45102857186508	36.0	36.0	36.0	27.0	36.0
25	32.87779868560763	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	10.0
5	39.0
6	72.0
7	14.0
8	47.0
9	78.0
10	48.0
11	9.0
12	30.0
13	18.0
14	173.0
15	196.0
16	298.0
17	433.0
18	635.0
19	893.0
20	1231.0
21	1709.0
22	2390.0
23	3111.0
24	3854.0
25	4672.0
26	5673.0
27	6761.0
28	8754.0
29	11409.0
30	14734.0
31	19856.0
32	27945.0
33	39532.0
34	84294.0
35	153809.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.323055846275686	19.484444987687898	13.315406299417118	21.877092866619304
2	11.67778569973518	23.59161743735995	40.765430841311876	23.965166021592992
3	18.628314753230086	30.62483130216901	29.97066567527514	20.77618826932577
4	10.235540694372643	19.089772353955347	38.551102576966464	32.123584374705544
5	9.432318637177564	42.15824369518871	36.29217747461137	12.11726019302236
6	27.40764516178336	37.86994156337088	21.388723309948492	13.333689964897264
7	24.428607100814855	35.3035453113932	23.625726821959834	16.642120765832104
8	27.234197635953084	33.55310440442634	23.087685853946958	16.125012105673612
9	26.532401531254617	17.158054633778335	21.30656186441974	35.00298197054731
10	15.023109141940862	29.012488878805105	35.24163265514226	20.72276932411177
11	31.022132263343856	24.674593018216672	24.20808712188805	20.095187596551426
12	21.308027097797346	28.884344826355335	31.67953820539559	18.128089870451724
13	29.120897329679433	22.957364094066662	27.78586450831687	20.135874067937035
14	22.575217584165685	23.26914154315228	28.20665779504515	25.948983077636882
15	24.42841884427042	28.010216957396207	30.511179258148967	17.050184940184405
16	22.470105710185344	28.216233026339605	30.13364805926092	19.180013204214134
17	19.3163190646996	27.998337832911417	32.4099638249218	20.275379277467184
18	21.234881396724386	25.361010830324908	35.61310652878908	17.79100124416162
19	20.174488123130892	33.26925577399148	29.159963593068404	17.39629250980922
20	19.41165673262733	26.53765162369281	36.08758610492181	17.96310553875805
21	20.965706433198804	28.92785118973258	27.211260711162556	22.895181665906062
22	18.69337379352552	31.420229246088923	28.33955961290816	21.546837347477403
23	18.942073069676006	30.439749006249343	28.625373214244735	21.992804709829908
24	18.298798879191878	34.02835675149216	28.493410535494384	19.179433833821584
25	22.19576368846691	28.841643125936955	28.17799851107009	20.78459467452604
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	224.0
1	224.0
2	573.0
3	922.0
4	922.0
5	922.0
6	3192.0
7	5462.0
8	5462.0
9	5462.0
10	5016.5
11	4571.0
12	4571.0
13	4571.0
14	3756.0
15	2941.0
16	2941.0
17	2941.0
18	4863.0
19	6785.0
20	6785.0
21	6785.0
22	10332.0
23	13879.0
24	13879.0
25	13879.0
26	20337.5
27	26796.0
28	26796.0
29	26796.0
30	31138.5
31	35481.0
32	35481.0
33	35481.0
34	36055.0
35	36629.0
36	36629.0
37	36629.0
38	35011.0
39	33393.0
40	33393.0
41	33393.0
42	37055.5
43	40718.0
44	40718.0
45	40718.0
46	66932.0
47	93146.0
48	93146.0
49	93146.0
50	69964.0
51	46782.0
52	46782.0
53	46782.0
54	33015.0
55	19248.0
56	19248.0
57	19248.0
58	14688.5
59	10129.0
60	10129.0
61	10129.0
62	8149.0
63	6169.0
64	6169.0
65	6169.0
66	5134.5
67	4100.0
68	4100.0
69	4100.0
70	3288.5
71	2477.0
72	2477.0
73	2477.0
74	2019.0
75	1561.0
76	1561.0
77	1561.0
78	1116.5
79	672.0
80	672.0
81	672.0
82	456.0
83	240.0
84	240.0
85	240.0
86	164.0
87	88.0
88	88.0
89	88.0
90	58.5
91	29.0
92	29.0
93	29.0
94	24.5
95	20.0
96	20.0
97	20.0
98	143.5
99	267.0
100	267.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.006620341253128748
2	0.0022916565876214896
3	0.0038194276460358162
4	0.014768453564671822
5	0.03361096328511518
6	0.04252296112586542
7	0.06798581209943753
8	0.0893746069172381
9	0.09446717711195252
10	0.1171291144784317
11	0.1148374578908102
12	0.13240682506257495
13	0.11560134342001736
14	0.11891151404658175
15	0.11203654428371726
16	0.10949025918636007
17	0.11967539957578889
18	0.12655036933865338
19	0.12629574082891765
20	0.12094854212446751
21	0.12680499784838908
22	0.1461567645883039
23	0.13444385314046073
24	0.1303697969846892
25	0.12756888337759625
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	392729.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.08683850696027
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.029514990063	17.59050133807282
2	13.298659562772212	6.406453304950742
3	4.759186434944395	3.4390126524906486
4	2.1924817117002835	2.112398116767542
5	1.2886379973783246	1.55196076683922
6	0.8097593978603747	1.170272630745374
7	0.5454776100469364	0.9197181771654245
8	0.4313078777115311	0.8311074557773936
9	0.3393378155524547	0.7356217646264982
>10	2.4313924478836313	11.87714683662271
>50	0.4186223518964861	7.167028663531341
>100	0.38162290160260476	19.36373427987238
>500	0.04651359465516512	7.543624229430472
>1k	0.025371051630090066	13.97783204194241
>5k	0.0010571271512537527	2.4240634126840646
>10k+	0.0010571271512537527	2.8895243284809626
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	11348	2.8895243284809626	No Hit
TCCATGTACTCTGCGTTGATACCAC	9520	2.4240634126840646	No Hit
GAGTACATGGAAGCAGTGGTATCAA	4847	1.23418438668904	No Hit
CATGTACTCTGCGTTGATACCACTG	4260	1.0847174514741718	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3785	0.9637689093497043	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3685	0.9383060583761321	No Hit
CCCATGTACTCTGCGTTGATACCAC	2936	0.747589304584077	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2818	0.7175431404352619	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2782	0.708376514084776	No Hit
GCTTCCATGTACTCTGCGTTGATAC	2721	0.692844174990897	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2592	0.659997097234989	No Hit
GCGTTGATACCACTGCTTCCATGTA	2572	0.6549045270402746	No Hit
CATGGAAGCAGTGGTATCAACGCAG	2504	0.6375897883782455	No Hit
GTACATGGGGTGGTATCAACGCAAA	1957	0.4983079935528061	No Hit
GTATCAACGCAGAGTACATGGAAGC	1919	0.48863211018284874	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1863	0.47437291363764833	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1834	0.4669886868553124	No Hit
CAGTGGTATCAACGCAGAGTACATG	1664	0.4237018402002399	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	1606	0.40893338663556805	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1414	0.3600447127663096	No Hit
GTATCAACGCAGAGTACATGGGAAG	1290	0.32847077755908016	No Hit
TATCAACGCAGAGTACATGGAAGCA	1259	0.32057729375727284	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	1186	0.3019894125465652	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	1184	0.30148015552709373	No Hit
GTACATGGGTGGTATCAACGCAAAA	1171	0.2981699849005294	No Hit
GAGTACATGGGAAGCAGTGGTATCA	1046	0.26634142118356424	No Hit
CTCTAATACTTGTAATGCTAGAGGT	983	0.2502998250702138	No Hit
ATACCACTGCTTCCATGTACTCTGC	943	0.24011468468078498	No Hit
GCGTTGATACCACTGCTTCCCATGT	932	0.23731377107369203	No Hit
GGTATCAACGCAGAGTACATGGAAG	931	0.2370591425639563	No Hit
GTACATGGGAGTGGTATCAACGCAA	906	0.2306934298205633	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	867	0.2207629179408702	No Hit
TATCAACGCAGAGTACATGGGAAGC	842	0.21439720519747713	No Hit
CCATTGGGATGTCCTGATCCAACAT	835	0.21261480562932708	No Hit
GTACTCTGCGTTGATACCACTGCTT	812	0.2067583499054055	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	796	0.20268429374963398	No Hit
GATATATTTTGATCAACGGACCAAG	752	0.19148063932126225	No Hit
ATCTATAACTTTATAGATGCAACAC	752	0.19148063932126225	No Hit
GTCAGGATACCGCGGCCGTTAAACT	724	0.18435104104866207	No Hit
CTTCTACACCATTGGGATGTCCTGA	717	0.18256864148051202	No Hit
CCACTGCTTCCATGTACTCTGCGTT	704	0.17925847085394764	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	696	0.17722144277606186	No Hit
GTGGTATCAACGCAGAGTACATGGA	689	0.17543904320791182	No Hit
GGTATCAACGCAGAGTACATGGGAA	674	0.171619615561876	No Hit
GCTTCCCATGTACTCTGCGTTGATA	668	0.17009184450346168	No Hit
GTATCAACGCAGAGTACATGGGGTG	666	0.16958258748399024	No Hit
ATCCTGACCGTGCAAAGGTAGCATA	655	0.1667816738768973	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	646	0.16449001728927581	No Hit
CTGCTTCCATGTACTCTGCGTTGAT	626	0.15939744709456138	No Hit
GTTATATAATTTAAGCTCCATAGGG	623	0.15863356156535421	No Hit
CCTCTAGCATTACAAGTATTAGAGG	618	0.1573604190166756	No Hit
CTGTTAGTATGAGTAACAAGAATTC	615	0.15659653348746846	No Hit
GTTATAGATTAACCCAATTTTAAGT	610	0.15532339093878986	No Hit
AAAAAGTACTCTGCGTTGATACCAC	603	0.1535409913706398	No Hit
GTATCCTGACCGTGCAAAGGTAGCA	601	0.15303173435116837	No Hit
ACATGGAAGCAGTGGTATCAACGCA	588	0.149721563724604	No Hit
GCCTAAAGGAAAGATCCAAAAAGAT	578	0.14717527862724678	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	556	0.1415734514130609	No Hit
GTTGATACCACTGCTTCCATGTACT	556	0.1415734514130609	No Hit
GTCCTACAGTGGACATTTCTAAATT	554	0.14106419439358947	No Hit
TGGAAGCAGTGGTATCAACGCAGAG	554	0.14106419439358947	No Hit
ATCGTAAATAGATAGAAACCGACCT	552	0.14055493737411803	No Hit
GTTCATGCTAGTCCCTAATTAAGGA	551	0.1403003088643823	No Hit
GGTCAGGATACCGCGGCCGTTAAAC	550	0.1400456803546466	No Hit
ACCTATAACTTCTCTGTTAACCCAA	532	0.1354623671794036	No Hit
GATATACACTGTTCTACAAATCCCG	520	0.13240682506257495	No Hit
TATCAACGCAGAGTACATGGGGTGG	518	0.1318975680431035	No Hit
GATACCACTGCTTCCATGTACTCTG	513	0.1306244254944249	No Hit
ATAAATAATCCACCTATAACTTCTC	512	0.1303697969846892	No Hit
TGCTAGAGGTGATGTTTTTGGTAAA	506	0.12884202592627486	No Hit
CTATAGAACTAGTACCGCAAGGGAA	500	0.12731425486786052	No Hit
CACTATAAATAATCCACCTATAACT	495	0.12604111231918194	No Hit
GATTAAACCTTGTACCTTTTGCATA	492	0.12527722678997477	No Hit
CCTATAACTTCTCTGTTAACCCAAC	477	0.12145779914393895	No Hit
GTACATGGGAGAAATCGTAAATAGA	477	0.12145779914393895	No Hit
GATTAAAGATAAGAGACAGTTGGAC	474	0.12069391361473178	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	468	0.11916614255631747	No Hit
GCAGAGTACATGGAAGCAGTGGTAT	467	0.11891151404658175	No Hit
CTTTAGGACGTGAAATATGGCGAGG	465	0.1184022570271103	No Hit
GTGTAGAAGCTATTAATGGTTCGTT	463	0.11789300000763885	No Hit
GACTATAGGCAATAATCACACTATA	463	0.11789300000763885	No Hit
TTGTAGAACAGTGTATATCAATGAG	459	0.11687448596869596	No Hit
AAATATATCTGGGTCAATAAGATAT	455	0.11585597192975308	No Hit
CCCCATGTACTCTGCGTTGATACCA	451	0.1148374578908102	No Hit
CTGTAGGACGTGGAATATGGCAAGA	451	0.1148374578908102	No Hit
AGCATGAACGGCTAAACGAGGGTCC	449	0.11432820087133877	No Hit
TACTAACAGTGTTGCATCTATAAAG	447	0.11381894385186732	No Hit
TCCCAATGGTGTAGAAGCTATTAAT	446	0.1135643153421316	No Hit
ATCCTAGCCCTAGCCCTACACAAAT	446	0.1135643153421316	No Hit
GATAGAAACCGACCTGGATTGCTCC	443	0.11280042981292443	No Hit
GTCCTGATCCAACATCGAGGTCGTA	437	0.1112726587545101	No Hit
CCTATAGTCTGATTAACTAACAATG	433	0.11025414471556721	No Hit
GTCCTACAGTGTGCATTTCTCATTT	430	0.10949025918636007	No Hit
GCAGTGGTATCAACGCAGAGTACAT	428	0.10898100216688862	No Hit
ACCCTATGGAGCTTAAATTATATAA	426	0.10847174514741717	No Hit
GTATCAACGCAGAGTACATGGGAGA	426	0.10847174514741717	No Hit
CTGTTAACCCAACACCGGAATGCCT	414	0.10541620303058852	No Hit
CCAATAAAGAAAGCGTTCAAGCTCA	408	0.1038884319721742	No Hit
GTGTATATCAATGAGTTACAATGAA	408	0.1038884319721742	No Hit
GTTAGTATGAGTAACAAGAATTCCA	396	0.10083288985534554	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.546285097357211E-4	0.0
3	0.0	0.0	0.0	2.546285097357211E-4	0.0
4	0.0	0.0	0.0	2.546285097357211E-4	0.0
5	0.0	0.0	0.0	2.546285097357211E-4	0.0
6	0.0	0.0	0.0	2.546285097357211E-4	0.0
7	0.0	0.0	0.0	2.546285097357211E-4	0.0
8	0.0	0.0	0.0	5.092570194714422E-4	0.0
9	0.0	0.0	0.0	7.638855292071632E-4	0.0
10	0.0	0.0	0.0	0.0017823995681500475	0.0
11	0.0	0.0	0.0	0.0017823995681500475	0.0
12	0.0	0.0	0.0	0.0017823995681500475	0.0
13	0.0	0.0	0.0	0.0020370280778857688	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCCCAC	25	0.006020947	19.001785	11
CTCGGCA	60	7.633571E-8	17.418303	12
TTGAACT	45	3.5290286E-5	16.888323	9
AAGCGTT	75	3.252353E-9	16.468214	11
AAAGCGT	75	3.252353E-9	16.468214	10
GAAAGCG	75	3.25781E-9	16.466114	9
CCAATTT	105	0.0	16.287243	14
ACTCGGC	65	1.9005711E-7	16.078432	11
TCCAACG	65	1.9005711E-7	16.078432	18
AACGGAC	125	0.0	15.9615	15
GGACCAA	125	0.0	15.9615	18
CGGACCA	125	0.0	15.9615	17
GGCAAAC	60	1.4585676E-6	15.834821	15
GCGTTCA	85	1.0095391E-9	15.648529	13
GTTTAAA	55	1.1284645E-5	15.53899	1
AGCGTTC	80	7.6452125E-9	15.4389515	12
GAACTCG	80	7.657945E-9	15.436982	9
ACGGACC	130	0.0	15.347595	16
GAACTTA	50	8.680534E-5	15.201428	11
TGAACTT	50	8.680534E-5	15.201428	10
>>END_MODULE