FastQCFastQC Report
Thu 2 Feb 2017
SRR4062460_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062460_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1402883
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68590.4889217418701346No Hit
GTACTTTTTTTTTTTTTTTTTTTTT53370.3804308698587124No Hit
TATCAACGCAGAGTACTTTTTTTTT48730.34735612306942204No Hit
GAGTACTTTTTTTTTTTTTTTTTTT37120.26459797431432275No Hit
GTACATGGAAGCAGTGGTATCAACG31380.22368223151895064No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28710.204649995758734No Hit
TCCATGTACTCTGCGTTGATACCAC26110.18611673247163163No Hit
GGTATCAACGCAGAGTACTTTTTTT22200.15824555575910465No Hit
GTACATGGGGTGGTATCAACGCAAA21230.151331222917378No Hit
GATTAAGAGGGACGGCCGGGGGCAT14890.10613857320959767No Hit
GCAGAGTACTTTTTTTTTTTTTTTT14250.10157653916969556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG1450.014.4155537
CGCGGTC1500.013.93453810
CGAACGA1153.765308E-1013.22042116
GAACCGC2050.012.976736
CGAACGT1001.4457692E-712.3475414
AGAACCG2050.012.0489615
CGACCAT1750.011.94388910
GATATAC1850.011.8029851
CCGACCA2100.011.7629219
TAGAACG658.035966E-411.68998054
CGATAAC1406.7848305E-1011.53671110
GTTTTCG2250.011.40017115
TCTAGCA1001.9368908E-611.3961053
GACCATA1950.011.20569711
CCGATAA1408.3327905E-910.8580819
ACGAACG1408.341885E-910.857306515
TCGAACG1053.47903E-610.8534343
CGGTCCT1855.456968E-1210.785112
TCCATTA2300.010.7404418
TTTTCGG2400.010.68994916
Produced by FastQC (version 0.11.5)