FastQCFastQC Report
Thu 2 Feb 2017
SRR4062460_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062460_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1402883
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA19700.14042511029073698No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14830.10571088251835684No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14810.1055683189546099No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGCGT500.00258071621.9998938
ATCGATT550.004484091419.99990329
ATCGTTT4250.019.67049229
ATACCGT3400.019.411676
CGCCGTT808.9902154E-519.24990725
CGTCGTA3450.019.13034210
ATAATAC1701.2732926E-1018.117563
ACCGTCG3650.018.0821048
CATCGTT4850.017.2370328
TGGTCGG4550.016.92299537
TTAGAGT3650.016.876634
TACCGTC3800.016.789397
CCGTCGT3750.016.4265869
ATAACGC5000.016.279923
CGTTTAT5000.016.2799231
GTCGTAG3800.016.21044711
CGCAATA4350.016.1838336
GGACCGT1501.8382889E-716.1332556
GACGGTA4000.015.9499238
GATATAC2353.6379788E-1215.9176511
Produced by FastQC (version 0.11.5)