##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062460_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1402883
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.09462870389049	32.0	32.0	32.0	32.0	32.0
2	31.374405420836947	32.0	32.0	32.0	32.0	32.0
3	31.475458038909874	32.0	32.0	32.0	32.0	32.0
4	31.58873049284937	32.0	32.0	32.0	32.0	32.0
5	31.51810664182259	32.0	32.0	32.0	32.0	32.0
6	35.09317455554027	36.0	36.0	36.0	36.0	36.0
7	35.1108488733558	36.0	36.0	36.0	36.0	36.0
8	35.06162951578998	36.0	36.0	36.0	36.0	36.0
9	35.16774028910465	36.0	36.0	36.0	36.0	36.0
10	35.05451202986992	36.0	36.0	36.0	36.0	36.0
11	35.17631833873531	36.0	36.0	36.0	36.0	36.0
12	35.08206600265311	36.0	36.0	36.0	36.0	36.0
13	35.122391532294564	36.0	36.0	36.0	36.0	36.0
14	35.07481023007621	36.0	36.0	36.0	36.0	36.0
15	35.05390684754181	36.0	36.0	36.0	36.0	36.0
16	35.06721301776413	36.0	36.0	36.0	36.0	36.0
17	35.04550842800148	36.0	36.0	36.0	36.0	36.0
18	35.05486843877929	36.0	36.0	36.0	36.0	36.0
19	35.01742055467206	36.0	36.0	36.0	36.0	36.0
20	34.98736530416293	36.0	36.0	36.0	36.0	36.0
21	34.9764827145243	36.0	36.0	36.0	36.0	36.0
22	34.97050644993203	36.0	36.0	36.0	36.0	36.0
23	34.94563552341856	36.0	36.0	36.0	32.0	36.0
24	34.9277088680952	36.0	36.0	36.0	32.0	36.0
25	34.90575265364254	36.0	36.0	36.0	32.0	36.0
26	34.86147027228928	36.0	36.0	36.0	32.0	36.0
27	34.84828029137141	36.0	36.0	36.0	32.0	36.0
28	34.83359980839457	36.0	36.0	36.0	32.0	36.0
29	34.808103027836246	36.0	36.0	36.0	32.0	36.0
30	34.78468767530863	36.0	36.0	36.0	32.0	36.0
31	34.77935365957104	36.0	36.0	36.0	32.0	36.0
32	34.73198406424484	36.0	36.0	36.0	32.0	36.0
33	34.687939764043044	36.0	36.0	36.0	32.0	36.0
34	34.65261465140001	36.0	36.0	36.0	32.0	36.0
35	34.611246269289744	36.0	36.0	36.0	32.0	36.0
36	34.58114753689367	36.0	36.0	36.0	32.0	36.0
37	34.553511590061326	36.0	36.0	36.0	32.0	36.0
38	34.52446497676571	36.0	36.0	36.0	32.0	36.0
39	34.47951753638757	36.0	36.0	36.0	32.0	36.0
40	34.4557272416873	36.0	36.0	36.0	32.0	36.0
41	34.41125453797644	36.0	36.0	36.0	32.0	36.0
42	34.366451799615504	36.0	36.0	36.0	32.0	36.0
43	34.3465028801404	36.0	36.0	36.0	32.0	36.0
44	34.302679553462404	36.0	36.0	36.0	32.0	36.0
45	34.22147677318779	36.0	36.0	36.0	32.0	36.0
46	34.21191931187419	36.0	36.0	36.0	32.0	36.0
47	34.14410325023541	36.0	36.0	36.0	32.0	36.0
48	34.07736069223164	36.0	36.0	36.0	32.0	36.0
49	34.03418104004397	36.0	36.0	36.0	32.0	36.0
50	33.50580055499995	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	5.0
20	13.0
21	50.0
22	138.0
23	432.0
24	1167.0
25	2754.0
26	5673.0
27	10573.0
28	17901.0
29	26044.0
30	36138.0
31	49546.0
32	71720.0
33	112992.0
34	238584.0
35	829152.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.023398233369214	18.428354495355286	11.888897602429651	25.65934966884584
2	16.055416353313113	21.126932247912265	36.581137482312606	26.236513916462023
3	18.25604222177552	25.57827551647399	28.812697896752248	27.35298436499824
4	12.359129278157393	16.09401218349084	35.82952807149853	35.717330466853234
5	14.145584485662738	37.228193655493726	32.997548619521375	15.628673239322167
6	33.31301790964983	35.56852891383765	17.305176868929877	13.813276307582642
7	29.279490877001145	30.962239901688164	21.438993843392502	18.31927537791819
8	28.562253587790288	31.917772187702038	19.968521965124676	19.551452259382998
9	28.01074644143525	13.712904069690774	19.149351727834752	39.126997761039235
10	16.340920803801882	26.783701848265324	31.401121832683128	25.474255515249666
11	37.10387822790639	21.341266520443973	22.309130554721953	19.245724696927685
12	24.587937839434932	24.217914109729747	28.886086722841466	22.30806132799385
13	29.851242049408256	19.65039137262338	25.697153647168008	24.80121293080036
14	23.912329110838183	19.599638743929464	25.685107026031396	30.802925119200957
15	25.38101894455917	26.715342619448663	23.362390163684353	24.54124827230781
16	25.253638400351274	25.689597778289425	24.47203366210867	24.58473015925063
17	23.48841635403665	25.635780032974953	25.939226578410317	24.936577034578082
18	24.520719119128252	24.535617011539806	27.34376280844518	23.59990106088676
19	25.125901447234018	25.096675916665895	26.335553285626812	23.441869350473276
20	25.53612810191584	23.8158135781815	26.27204121797755	24.376017101925108
21	26.785555174594034	23.947185902174308	25.193832985359432	24.073425937872226
22	25.544468070395038	24.302881993722927	25.619456504925925	24.53319343095611
23	24.293045107824387	24.161744065613455	26.253080263999205	25.292130562562953
24	24.380721699528756	25.343667290857468	25.858892010238915	24.416718999374858
25	24.603691113228972	24.44074095986622	26.15471140501382	24.800856521890992
26	23.928367511759713	25.1815725188772	26.660812056315457	24.22924791304763
27	24.839776374793907	25.123335303086574	25.824320346030284	24.21256797608924
28	23.985321655476614	24.75537874505572	26.603715349034808	24.655584250432856
29	23.988671899224666	25.073438055775142	26.478116849373755	24.459773195626436
30	24.108710419899595	25.18228533669593	26.600293823504884	24.108710419899595
31	24.29404305277062	25.049701222411276	25.709556677213996	24.946699047604113
32	24.085258713663222	25.34487908114932	25.63428311555561	24.93557908963185
33	23.898144036245363	24.757588480293798	26.13418225183426	25.21008523162659
34	24.355773075873042	25.073509337557017	26.565864722859995	24.004852863709946
35	25.270532182655288	24.854888112551084	26.061617397887066	23.812962306906563
36	24.111704254738278	25.480385748490786	25.670993233220447	24.736916763550486
37	25.069588839553976	25.343453445511848	25.498348757522898	24.088608957411275
38	24.089036648102518	25.232467711134852	25.89531700077626	24.78317863998637
39	24.343726454736426	25.146430600413577	25.60035298738384	24.90948995746616
40	24.812190325208874	25.201602699583642	26.00252480071396	23.983682174493527
41	23.68886072466485	25.534631184496497	26.47640608660879	24.300102004229863
42	24.86679217012395	25.81583781398734	25.861030463695116	23.45633955219359
43	24.11519706205008	24.767425366192334	25.9142779547546	25.203099617002987
44	23.623851739596248	25.422433659827654	25.963177257119803	24.990537343456296
45	23.923163941682947	25.50982512440453	25.828312125815195	24.738698808097325
46	23.711188824149144	25.23341236112517	26.036045797151864	25.019353017573824
47	23.98517909191287	25.055617610306776	26.286582701479738	24.672620596300618
48	24.47039418112558	26.077228108117357	25.104588194453846	24.347789516303212
49	23.50645064485064	25.816336786460454	25.778129751376273	24.899082817312635
50	23.358469665681312	26.38053208998897	25.22284467058194	25.038153573747774
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	40.0
1	46.0
2	52.0
3	177.5
4	303.0
5	353.5
6	404.0
7	409.0
8	414.0
9	582.0
10	750.0
11	1112.5
12	1475.0
13	2587.5
14	3700.0
15	5158.0
16	6616.0
17	7941.0
18	9266.0
19	10058.5
20	10851.0
21	11543.0
22	12235.0
23	12528.5
24	12822.0
25	12799.5
26	12777.0
27	14461.5
28	16146.0
29	18127.5
30	20109.0
31	22360.0
32	24611.0
33	28105.5
34	31600.0
35	35595.5
36	39591.0
37	46286.5
38	52982.0
39	56350.0
40	59718.0
41	65241.0
42	70764.0
43	72462.0
44	74160.0
45	81901.5
46	89643.0
47	95168.0
48	100693.0
49	102276.0
50	103859.0
51	99273.5
52	94688.0
53	92377.0
54	90066.0
55	91021.5
56	91977.0
57	89912.5
58	87848.0
59	79807.0
60	71766.0
61	63940.0
62	56114.0
63	49329.0
64	42544.0
65	36949.5
66	31355.0
67	27570.5
68	23786.0
69	21504.5
70	19223.0
71	15500.0
72	11777.0
73	10571.0
74	9365.0
75	7180.0
76	4995.0
77	4458.0
78	3921.0
79	3403.0
80	2885.0
81	2353.0
82	1821.0
83	1673.5
84	1526.0
85	1249.0
86	972.0
87	711.5
88	451.0
89	294.5
90	138.0
91	90.5
92	43.0
93	29.0
94	15.0
95	14.0
96	13.0
97	11.5
98	10.0
99	6.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01518301953904923
2	0.003421525529926587
3	3.564089093673528E-4
4	7.128178187347056E-5
5	0.0
6	5.702542549877645E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	7.128178187347056E-5
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1402883.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.065290149748975
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.86081951797716	40.25576398719199
2	14.238629115826862	15.111539707320324
3	4.630621121516091	7.371757601604223
4	1.9046256744134091	4.042780561576357
5	0.9904393076181132	2.6278974617235833
6	0.5799581435145846	1.8465388296186718
7	0.3456807544806756	1.2840554674990834
8	0.2262278670786412	0.9603877925189553
9	0.16244833699689815	0.7758331323376141
>10	0.8725220042520061	8.550706142570025
>50	0.09193309895704456	3.4347446845470206
>100	0.08595847985961404	9.48994394210847
>500	0.007838953273634921	2.7605767095788627
>1k	0.0022976242353757526	1.4874739798047754
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1970	0.14042511029073698	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1483	0.10571088251835684	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1481	0.1055683189546099	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.128178187347056E-5
2	0.0	0.0	0.0	0.0	7.128178187347056E-5
3	0.0	0.0	0.0	0.0	7.128178187347056E-5
4	0.0	0.0	0.0	7.128178187347056E-5	7.128178187347056E-5
5	0.0	0.0	0.0	7.128178187347056E-5	7.128178187347056E-5
6	0.0	0.0	0.0	7.128178187347056E-5	7.128178187347056E-5
7	0.0	0.0	0.0	7.128178187347056E-5	7.128178187347056E-5
8	0.0	0.0	0.0	7.128178187347056E-5	7.128178187347056E-5
9	0.0	0.0	0.0	7.128178187347056E-5	7.128178187347056E-5
10	0.0	0.0	0.0	7.128178187347056E-5	7.128178187347056E-5
11	0.0	0.0	0.0	1.4256356374694112E-4	7.128178187347056E-5
12	0.0	0.0	0.0	1.4256356374694112E-4	2.8512712749388224E-4
13	0.0	0.0	0.0	1.4256356374694112E-4	2.8512712749388224E-4
14	0.0	0.0	0.0	2.1384534562041168E-4	2.8512712749388224E-4
15	0.0	0.0	0.0	2.1384534562041168E-4	2.8512712749388224E-4
16	0.0	0.0	0.0	3.564089093673528E-4	2.8512712749388224E-4
17	0.0	0.0	0.0	4.2769069124082336E-4	2.8512712749388224E-4
18	0.0	0.0	0.0	4.2769069124082336E-4	3.564089093673528E-4
19	0.0	0.0	0.0	5.702542549877645E-4	3.564089093673528E-4
20	0.0	0.0	0.0	7.840996006081762E-4	4.2769069124082336E-4
21	0.0	0.0	0.0	0.001140508509975529	4.2769069124082336E-4
22	0.0	0.0	0.0	0.0019246081105837051	4.2769069124082336E-4
23	0.0	0.0	0.0	0.0034928073118000572	6.41536036861235E-4
24	0.0	0.0	0.0	0.006058951459244998	6.41536036861235E-4
25	0.0	0.0	0.0	0.00762715066046135	7.840996006081762E-4
26	0.0	0.0	0.0	0.009052786297930762	8.553813824816467E-4
27	0.0	0.0	0.0	0.011761494009122643	8.553813824816467E-4
28	0.0	0.0	0.0	0.021241970998294227	8.553813824816467E-4
29	0.0	0.0	0.0	0.04291163268782928	8.553813824816467E-4
30	0.0	0.0	0.0	0.08354224835570749	8.553813824816467E-4
31	0.0	0.0	0.0	0.16409066187272922	8.553813824816467E-4
32	0.0	0.0	0.0	0.26417028362308187	8.553813824816467E-4
33	0.0	0.0	0.0	0.35754941787732836	8.553813824816467E-4
34	7.128178187347056E-5	0.0	0.0	0.46233363723133003	8.553813824816467E-4
35	7.128178187347056E-5	0.0	0.0	0.5783090963394667	8.553813824816467E-4
36	7.128178187347056E-5	0.0	0.0	0.7540186886575716	8.553813824816467E-4
37	7.128178187347056E-5	0.0	0.0	0.9951649567355225	8.553813824816467E-4
38	7.128178187347056E-5	0.0	0.0	1.2909843515104253	9.266631643551173E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTGCGT	50	0.002580716	21.999893	8
ATCGATT	55	0.0044840914	19.999903	29
ATCGTTT	425	0.0	19.670492	29
ATACCGT	340	0.0	19.41167	6
CGCCGTT	80	8.9902154E-5	19.249907	25
CGTCGTA	345	0.0	19.130342	10
ATAATAC	170	1.2732926E-10	18.11756	3
ACCGTCG	365	0.0	18.082104	8
CATCGTT	485	0.0	17.23703	28
TGGTCGG	455	0.0	16.922995	37
TTAGAGT	365	0.0	16.87663	4
TACCGTC	380	0.0	16.78939	7
CCGTCGT	375	0.0	16.426586	9
ATAACGC	500	0.0	16.27992	3
CGTTTAT	500	0.0	16.27992	31
GTCGTAG	380	0.0	16.210447	11
CGCAATA	435	0.0	16.18383	36
GGACCGT	150	1.8382889E-7	16.133255	6
GACGGTA	400	0.0	15.949923	8
GATATAC	235	3.6379788E-12	15.917651	1
>>END_MODULE