FastQCFastQC Report
Thu 2 Feb 2017
SRR4062459_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062459_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1684899
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG47770.2835184779621805No Hit
TCCATGTACTCTGCGTTGATACCAC42150.2501633629078064No Hit
GTATCAACGCAGAGTACTTTTTTTT34850.20683732378023847No Hit
TATCAACGCAGAGTACTTTTTTTTT25780.15300620393269862No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG24130.14321333207509768No Hit
GTCTTGCGCCGGTCCAAGAATTTCA24050.14273852616685034No Hit
GCGCAAGACGGACCAGAGCGAAAGC24000.14244177247419576No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23860.14161086213476298No Hit
GAATAGGACCGCGGTTCTATTTTGT22780.13520098237342415No Hit
GATTAAGAGGGACGGCCGGGGGCAT22470.13336110947896582No Hit
GAATAACGCCGCCGCATCGCCAGTC22280.1322334454468784No Hit
GTATCTGATCGTCTTCGAACCTCCG21550.12790084153412165No Hit
GAGTACATGGAAGCAGTGGTATCAA21520.1277227893185289No Hit
GAACTACGACGGTATCTGATCGTCT21170.12564551346994687No Hit
GTCCTATTCCATTATTCCTAGCTGC19750.11721770859855696No Hit
CTATTGGAGCTGGAATTACCGCGGC19590.1162680967820623No Hit
CATGTACTCTGCGTTGATACCACTG18520.10991756775925442No Hit
ATCAGATACCGTCGTAGTTCCGACC18200.10801834412626514No Hit
GGGTAGGCACACGCTGAGCCAGTCA18190.10795899338773421No Hit
CTCTTAATCATGGCCTCAGTTCCGA17940.10647522492446135No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG17350.10297353135113738No Hit
GCTTTGAACACTCTAATTTTTTCAA17150.10178651658051907No Hit
TCGTAGTTCCGACCATAAACGATGC17130.10166781510345725No Hit
CTTTAATATACGCTATTGGAGCTGG17060.10125235993374085No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGGT307.7523047E-418.9893382
GGGCTAA350.00217931916.2756081
CGAACGA1750.015.74618216
TAACGAA1950.014.1295113
ATAACGA2050.013.90413512
ACGAACG2000.013.7766815
CGATAAC2100.013.11986910
ATGCGCC604.0891528E-412.66783611
CCGATAA2100.012.66745959
CGCGCCA604.0901784E-412.667459510
TCCATTA3850.012.5848298
AACGAAC2200.012.52388314
AATTCCG2200.012.5220225
CGCATCG3800.012.25113113
GAACCGT550.00306787212.0913076
TTAGACC550.003070604812.0898694
TTAGTAC550.003076076312.0869953
TTCGGGA550.003081556212.0841242
GCATCGC3800.012.00110814
AGGCCCG4750.012.00075110
Produced by FastQC (version 0.11.5)