##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062459_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1684899
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.110587044089883	32.0	32.0	32.0	32.0	32.0
2	30.72346176239644	32.0	32.0	32.0	32.0	32.0
3	30.694740159499176	32.0	32.0	32.0	32.0	32.0
4	30.781763773377513	32.0	32.0	32.0	32.0	32.0
5	30.530828850868804	32.0	32.0	32.0	32.0	32.0
6	34.17507399553326	36.0	36.0	36.0	32.0	36.0
7	34.054842456432105	36.0	36.0	36.0	32.0	36.0
8	34.03592322151061	36.0	36.0	36.0	32.0	36.0
9	34.24226021856503	36.0	36.0	36.0	32.0	36.0
10	33.84340663743049	36.0	36.0	36.0	32.0	36.0
11	34.223151061280234	36.0	36.0	36.0	32.0	36.0
12	33.95903018519211	36.0	36.0	36.0	32.0	36.0
13	34.105099474805314	36.0	36.0	36.0	32.0	36.0
14	33.99022374634919	36.0	36.0	36.0	32.0	36.0
15	33.90705021487935	36.0	36.0	36.0	32.0	36.0
16	33.91977204568345	36.0	36.0	36.0	32.0	36.0
17	33.824762789935775	36.0	36.0	36.0	32.0	36.0
18	33.8477534855205	36.0	36.0	36.0	32.0	36.0
19	33.833714661828395	36.0	36.0	36.0	32.0	36.0
20	33.827369474372055	36.0	36.0	36.0	32.0	36.0
21	33.804625677859626	36.0	36.0	36.0	32.0	36.0
22	33.74166938196296	36.0	36.0	36.0	32.0	36.0
23	33.70859024784275	36.0	36.0	36.0	27.0	36.0
24	33.67424634948445	36.0	36.0	36.0	27.0	36.0
25	33.204819992177576	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	7.0
4	68.0
5	169.0
6	302.0
7	85.0
8	267.0
9	334.0
10	204.0
11	62.0
12	121.0
13	87.0
14	313.0
15	351.0
16	591.0
17	843.0
18	1095.0
19	1542.0
20	2441.0
21	3688.0
22	5591.0
23	8424.0
24	11767.0
25	16767.0
26	22638.0
27	29186.0
28	39089.0
29	51497.0
30	66338.0
31	89682.0
32	128107.0
33	181288.0
34	386132.0
35	635822.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.33496046335218	17.36146147298794	11.354039670791078	25.949538392868803
2	16.935326395009632	19.965456763085033	36.48941000507465	26.609806836830685
3	19.06066543172579	23.66219519939982	28.36397241028922	28.913166958585162
4	12.65993801632043	15.194252530367075	35.32903527584874	36.81677417746375
5	14.851027801889716	36.235968731928416	33.12501410103107	15.7879893651508
6	34.58620855509233	34.82271849260691	16.795055796274866	13.796017156025897
7	30.38315407353997	30.360465717121333	20.674853876887752	18.58152633245094
8	28.54853158884012	32.083637296859294	19.6158059449325	19.752025169368082
9	27.315767023007066	14.356573286233187	18.680483026006076	39.64717666475367
10	16.270883076166108	26.073524116038417	30.855669821288288	26.79992298650719
11	37.588275703056105	21.002902312579778	21.911746878409506	19.497075105954615
12	24.768926291766217	23.662827324703727	28.159493159314863	23.408753224215193
13	29.45943087102191	18.92158342554175	25.342260613674263	26.27672508976207
14	23.866157969490054	19.7527826805251	24.45240769463907	31.92865165534578
15	25.451765616077516	26.870806710095746	22.315269443109116	25.362158230717625
16	26.2624522040364	25.424095404754194	23.607447702971296	24.70600468823811
17	24.235545109598363	25.52350786071485	25.284551637661306	24.95639539202548
18	25.463863240873692	24.0064827933779	26.329959206184682	24.199694759563727
19	25.7070502659889	25.15462777897633	24.989822232286077	24.148499722748692
20	25.671965532610308	23.81868964492646	25.109196256128364	25.400148566334867
21	26.990791752395964	24.125442315624067	23.898237393781848	24.98552853819812
22	25.881092751173107	24.222040723158845	24.57400228023059	25.322864245437458
23	24.1557577036348	23.95872771525452	25.27303363774417	26.612480943366506
24	24.593503907759057	25.301358057710022	24.811209176547504	25.293928857983417
25	25.04686205498877	24.19425198413406	24.88455352972011	25.874332431157054
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	271.0
1	271.0
2	641.0
3	1011.0
4	1011.0
5	1011.0
6	2489.5
7	3968.0
8	3968.0
9	3968.0
10	4072.0
11	4176.0
12	4176.0
13	4176.0
14	4540.5
15	4905.0
16	4905.0
17	4905.0
18	7821.0
19	10737.0
20	10737.0
21	10737.0
22	17399.5
23	24062.0
24	24062.0
25	24062.0
26	35881.5
27	47701.0
28	47701.0
29	47701.0
30	58141.5
31	68582.0
32	68582.0
33	68582.0
34	85178.0
35	101774.0
36	101774.0
37	101774.0
38	115027.0
39	128280.0
40	128280.0
41	128280.0
42	147808.5
43	167337.0
44	167337.0
45	167337.0
46	197911.5
47	228486.0
48	228486.0
49	228486.0
50	225667.0
51	222848.0
52	222848.0
53	222848.0
54	204969.0
55	187090.0
56	187090.0
57	187090.0
58	174348.5
59	161607.0
60	161607.0
61	161607.0
62	145728.0
63	129849.0
64	129849.0
65	129849.0
66	108847.5
67	87846.0
68	87846.0
69	87846.0
70	66883.0
71	45920.0
72	45920.0
73	45920.0
74	36518.0
75	27116.0
76	27116.0
77	27116.0
78	22569.0
79	18022.0
80	18022.0
81	18022.0
82	12790.0
83	7558.0
84	7558.0
85	7558.0
86	5571.5
87	3585.0
88	3585.0
89	3585.0
90	2417.0
91	1249.0
92	1249.0
93	1249.0
94	743.5
95	238.0
96	238.0
97	238.0
98	459.5
99	681.0
100	681.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007893648224611683
2	0.0032049398806694054
3	0.004035850220102214
4	0.015549893495099706
5	0.037094211581821815
6	0.049023710026535716
7	0.07217049805359253
8	0.09395221909443831
9	0.0995311885163443
10	0.12327148392871026
11	0.12481460313051404
12	0.13763436265319168
13	0.12564551346994687
14	0.12873175187355443
15	0.11882017853889165
16	0.11656485047471687
17	0.12843499818089987
18	0.13597254197432607
19	0.13710020600641346
20	0.12754473710293615
21	0.13514163163489326
22	0.15508347978128065
23	0.1432726828136286
24	0.13941488480911912
25	0.1365660493596352
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1684899.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.12944861569773
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.52512616537743	37.47975347268034
2	11.998512611056299	11.309665671351581
3	3.4869217860381023	4.930101034261192
4	1.4879765149740303	2.8051005081533424
5	0.8198860261034616	1.9320388168985854
6	0.5140743990438724	1.4536825784630327
7	0.3420467927122391	1.128433371890698
8	0.25038068981320155	0.9440243083931387
9	0.19241004819370733	0.816136153154033
>10	1.1347919402521771	9.808529214824738
>50	0.10773940123767764	3.584797659354661
>100	0.11465493606345456	11.848160042100517
>500	0.017784629544289013	5.867317935172292
>1k	0.007694059590082481	6.092259233301916
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	4777	0.2835184779621805	No Hit
TCCATGTACTCTGCGTTGATACCAC	4215	0.2501633629078064	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3485	0.20683732378023847	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2578	0.15300620393269862	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2413	0.14321333207509768	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2405	0.14273852616685034	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2400	0.14244177247419576	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2386	0.14161086213476298	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2278	0.13520098237342415	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2247	0.13336110947896582	No Hit
GAATAACGCCGCCGCATCGCCAGTC	2228	0.1322334454468784	No Hit
GTATCTGATCGTCTTCGAACCTCCG	2155	0.12790084153412165	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2152	0.1277227893185289	No Hit
GAACTACGACGGTATCTGATCGTCT	2117	0.12564551346994687	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1975	0.11721770859855696	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1959	0.1162680967820623	No Hit
CATGTACTCTGCGTTGATACCACTG	1852	0.10991756775925442	No Hit
ATCAGATACCGTCGTAGTTCCGACC	1820	0.10801834412626514	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1819	0.10795899338773421	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	1794	0.10647522492446135	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG	1735	0.10297353135113738	No Hit
GCTTTGAACACTCTAATTTTTTCAA	1715	0.10178651658051907	No Hit
TCGTAGTTCCGACCATAAACGATGC	1713	0.10166781510345725	No Hit
CTTTAATATACGCTATTGGAGCTGG	1706	0.10125235993374085	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.935073853091491E-5
2	0.0	0.0	0.0	0.0	5.935073853091491E-5
3	0.0	0.0	0.0	0.0	5.935073853091491E-5
4	0.0	0.0	0.0	0.0	5.935073853091491E-5
5	0.0	0.0	0.0	0.0	5.935073853091491E-5
6	0.0	0.0	0.0	0.0	5.935073853091491E-5
7	0.0	0.0	0.0	0.0	5.935073853091491E-5
8	0.0	0.0	0.0	0.0	5.935073853091491E-5
9	0.0	0.0	0.0	0.0	5.935073853091491E-5
10	0.0	0.0	0.0	0.0	5.935073853091491E-5
11	0.0	0.0	0.0	0.0	5.935073853091491E-5
12	0.0	0.0	0.0	0.0	3.5610443118548943E-4
13	0.0	0.0	0.0	0.0	4.1545516971640435E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTAGGT	30	7.7523047E-4	18.989338	2
GGGCTAA	35	0.002179319	16.275608	1
CGAACGA	175	0.0	15.746182	16
TAACGAA	195	0.0	14.12951	13
ATAACGA	205	0.0	13.904135	12
ACGAACG	200	0.0	13.77668	15
CGATAAC	210	0.0	13.119869	10
ATGCGCC	60	4.0891528E-4	12.667836	11
CCGATAA	210	0.0	12.6674595	9
CGCGCCA	60	4.0901784E-4	12.6674595	10
TCCATTA	385	0.0	12.584829	8
AACGAAC	220	0.0	12.523883	14
AATTCCG	220	0.0	12.522022	5
CGCATCG	380	0.0	12.251131	13
GAACCGT	55	0.003067872	12.091307	6
TTAGACC	55	0.0030706048	12.089869	4
TTAGTAC	55	0.0030760763	12.086995	3
TTCGGGA	55	0.0030815562	12.084124	2
GCATCGC	380	0.0	12.001108	14
AGGCCCG	475	0.0	12.000751	10
>>END_MODULE