FastQCFastQC Report
Thu 2 Feb 2017
SRR4062459_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062459_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1684899
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC24310.14428164536865415No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA23780.14113605622651565No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT23410.1389400789008718No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT23400.1388807281623409No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA23050.13680345231375887No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA22340.1325895498780639No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA20760.12321213319017935No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC20290.12042264847922635No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG19880.11798926819945885No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG19410.11519978348850583No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC19400.11514043274997493No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT18350.10890860520422886No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC18050.10712808304830142No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA17680.10493210572265757No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT16960.1006588525484317No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAC951.597319E-518.5268541
CGAGCCG7500.017.59998915
TAGTACG1002.4839683E-517.5999892
TAGGACG2251.8189894E-1216.6222114
ACCGTAT800.001988797716.4999898
TCGACGC1206.4095875E-616.49998914
CGCTTAC1206.4095875E-616.49998918
ATTACAC2151.2732926E-1116.3720823
TATACAG2700.016.2962865
TACGACG7100.016.1126655
GATATAC2750.016.0004631
CCGTCGT5650.015.964599
CGACGGT7050.015.9148837
GACGGTA7050.015.9148838
CTACGAC7350.015.86393454
ACGACGG7100.015.8028066
TAATACT3950.015.5949274
ACCGTCG5800.015.5517148
TCAAAGC11650.015.4849673
AGGCCCG9100.015.47251710
Produced by FastQC (version 0.11.5)