FastQCFastQC Report
Thu 2 Feb 2017
SRR4062458_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062458_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1960067
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33810.17249410351788996No Hit
TATCAACGCAGAGTACTTTTTTTTT24100.12295498062056041No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21410.10923095996208292No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTTAA350.002181214516.273681
AACCGCG1750.013.0295887
CGAACGA1457.2759576E-1212.45020216
AATCGCT1700.012.29570515
ACGAACG1405.2750693E-1112.21586315
CTTATAC1800.012.1348753
ACTGCGC550.0030677312.0915438
CCGTCGT2150.011.9311639
CGTTATT1850.011.8053982
ATCGCCA3300.011.80485516
GTATTAG4050.011.71972751
TAACGAA1552.5465852E-1111.64666513
GCATCGC3450.011.56671114
AAATCGC1657.2759576E-1211.51663814
ATTTAGA1900.011.4915011
AGAACCG2400.011.4783035
CGGTCCA4250.011.40118110
TCCAATA2600.011.3217322
TCGCCAG3450.011.29246717
CGCATCG3450.011.29131313
Produced by FastQC (version 0.11.5)