##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062458_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1960067
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.167394788035306	32.0	32.0	32.0	32.0	32.0
2	30.808440731872942	32.0	32.0	32.0	32.0	32.0
3	30.790058196990206	32.0	32.0	32.0	32.0	32.0
4	30.867205559809946	32.0	32.0	32.0	32.0	32.0
5	30.631354438394197	32.0	32.0	32.0	32.0	32.0
6	34.29679036481916	36.0	36.0	36.0	32.0	36.0
7	34.17828523208645	36.0	36.0	36.0	32.0	36.0
8	34.16636778232581	36.0	36.0	36.0	32.0	36.0
9	34.353845557320234	36.0	36.0	36.0	32.0	36.0
10	33.989885549830696	36.0	36.0	36.0	32.0	36.0
11	34.33155448257636	36.0	36.0	36.0	32.0	36.0
12	34.09329477002572	36.0	36.0	36.0	32.0	36.0
13	34.23017937652131	36.0	36.0	36.0	32.0	36.0
14	34.12501868558575	36.0	36.0	36.0	32.0	36.0
15	34.05439099785875	36.0	36.0	36.0	32.0	36.0
16	34.0575046669323	36.0	36.0	36.0	32.0	36.0
17	33.98515458910333	36.0	36.0	36.0	32.0	36.0
18	33.993429816429746	36.0	36.0	36.0	32.0	36.0
19	33.97990476856148	36.0	36.0	36.0	32.0	36.0
20	33.97458811356959	36.0	36.0	36.0	32.0	36.0
21	33.95623159820557	36.0	36.0	36.0	32.0	36.0
22	33.90679706356977	36.0	36.0	36.0	32.0	36.0
23	33.87490019473824	36.0	36.0	36.0	32.0	36.0
24	33.847518477684694	36.0	36.0	36.0	32.0	36.0
25	33.38884793223905	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	11.0
4	70.0
5	181.0
6	326.0
7	97.0
8	290.0
9	332.0
10	250.0
11	81.0
12	150.0
13	123.0
14	334.0
15	430.0
16	627.0
17	858.0
18	1091.0
19	1630.0
20	2456.0
21	3654.0
22	5553.0
23	8435.0
24	11930.0
25	17028.0
26	23803.0
27	30655.0
28	42319.0
29	56631.0
30	72489.0
31	99278.0
32	142065.0
33	204598.0
34	444944.0
35	787348.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.072513755775795	17.573156644407074	11.133096314921548	26.221233284895582
2	16.93670877034372	19.882775689629046	36.99147399264185	26.18904154738539
3	19.05597919976653	23.727256122262595	28.403124933034963	28.813639744935916
4	12.641221280562757	15.165689600715183	35.996150586367136	36.19693853235492
5	14.688406854814426	36.40933722980001	33.71103745977047	15.191218455615088
6	34.29933813775126	35.28526635527614	16.859443978543965	13.555951528428633
7	30.26787688006727	30.480152626159335	20.869135933566575	18.382834560206824
8	28.072975868411405	32.83536208616864	19.682630137881663	19.4090319075383
9	27.39714344661632	14.348083204471127	18.772533863353633	39.48223948555892
10	15.944971579648495	26.52930503965773	31.750752904351522	25.77497047634225
11	37.239889545319414	21.254148946277468	22.36656202144733	19.139399486955785
12	24.508443612525646	23.847534759402002	28.785014835582512	22.859006792489843
13	29.295042685446592	19.499329057211728	25.40214425857374	25.80348399876794
14	23.29940668810388	19.87592297927878	25.130526063070356	31.69414426954698
15	25.14574780088608	27.290209486606116	22.34295655300705	25.221086159500754
16	25.71184719674094	25.718997846632863	23.953451312246408	24.615703644379792
17	23.966159892566548	25.885730894320158	25.445923919012177	24.70218529410112
18	24.684189661936834	24.891187961812594	26.44347086902504	23.98115150722553
19	25.419641972268735	25.235529772127947	25.399667535461013	23.945160720142304
20	25.38926694911358	24.589483126231706	25.207007979959688	24.814241944695027
21	26.370256470510107	24.401198446004795	24.52518090353429	24.703364179950803
22	25.56075143604894	24.69835563326667	24.93273230984934	24.80816062083505
23	24.26739786383343	24.492192968204776	25.598542510286933	25.64186665767486
24	24.594505536610598	25.297418802763833	25.24628018953472	24.861795471090847
25	24.649235329186226	24.82205701225384	25.488363016466337	25.040344642093597
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	251.0
1	251.0
2	651.0
3	1051.0
4	1051.0
5	1051.0
6	2712.0
7	4373.0
8	4373.0
9	4373.0
10	4704.0
11	5035.0
12	5035.0
13	5035.0
14	5724.5
15	6414.0
16	6414.0
17	6414.0
18	10352.5
19	14291.0
20	14291.0
21	14291.0
22	22533.0
23	30775.0
24	30775.0
25	30775.0
26	45435.5
27	60096.0
28	60096.0
29	60096.0
30	74003.5
31	87911.0
32	87911.0
33	87911.0
34	107337.5
35	126764.0
36	126764.0
37	126764.0
38	142960.0
39	159156.0
40	159156.0
41	159156.0
42	180612.0
43	202068.0
44	202068.0
45	202068.0
46	227997.5
47	253927.0
48	253927.0
49	253927.0
50	255213.5
51	256500.0
52	256500.0
53	256500.0
54	239467.5
55	222435.0
56	222435.0
57	222435.0
58	203342.5
59	184250.0
60	184250.0
61	184250.0
62	162535.0
63	140820.0
64	140820.0
65	140820.0
66	118061.5
67	95303.0
68	95303.0
69	95303.0
70	72782.5
71	50262.0
72	50262.0
73	50262.0
74	39182.5
75	28103.0
76	28103.0
77	28103.0
78	22728.5
79	17354.0
80	17354.0
81	17354.0
82	12320.5
83	7287.0
84	7287.0
85	7287.0
86	5365.5
87	3444.0
88	3444.0
89	3444.0
90	2291.5
91	1139.0
92	1139.0
93	1139.0
94	693.5
95	248.0
96	248.0
97	248.0
98	529.0
99	810.0
100	810.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008316042257739149
2	0.0032651945061061686
3	0.004540661110053891
4	0.01469337527747776
5	0.03494778494816759
6	0.04581476041380218
7	0.0660181514203341
8	0.0878031210157612
9	0.09708851789250061
10	0.11810820752555908
11	0.1194346927936647
12	0.1332097321163001
13	0.1211183087108757
14	0.12305701794887625
15	0.11392467706461055
16	0.11239411713987327
17	0.12193460733740225
18	0.1307608362367205
19	0.1308628735650363
20	0.12310803661303414
21	0.1291792576478253
22	0.15106626457156822
23	0.13922993448693335
24	0.1347913107051953
25	0.13050574291593092
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1960067.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.22296740670641
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.4436369886382	42.28226102116507
2	12.707830603564732	13.526969080469438
3	3.5208998249628163	5.621782098788229
4	1.4221823591480804	3.027710613893245
5	0.7329198347798547	1.9504084239108428
6	0.444824424272496	1.4204925500857202
7	0.2841523482709696	1.0586401819395435
8	0.2049647097877764	0.872706405484789
9	0.15496071593667318	0.7422722220255702
>10	0.8895458852706357	8.76521892860282
>50	0.0878364992070271	3.2684980348237933
>100	0.09039672428597986	10.172442666419174
>500	0.011910825166454532	4.160622188855707
>1k	0.003938256708263192	3.129975583536098
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3381	0.17249410351788996	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2410	0.12295498062056041	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2141	0.10923095996208292	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.550933207895444E-4
2	0.0	0.0	0.0	0.0	2.550933207895444E-4
3	0.0	0.0	0.0	0.0	2.550933207895444E-4
4	0.0	0.0	0.0	0.0	2.550933207895444E-4
5	0.0	0.0	0.0	0.0	3.061119849474533E-4
6	0.0	0.0	0.0	0.0	3.571306491053622E-4
7	0.0	0.0	0.0	5.101866415790889E-5	3.571306491053622E-4
8	0.0	0.0	0.0	1.0203732831581778E-4	3.571306491053622E-4
9	0.0	0.0	0.0	1.5305599247372666E-4	3.571306491053622E-4
10	0.0	0.0	0.0	2.550933207895444E-4	3.571306491053622E-4
11	0.0	0.0	0.0	2.550933207895444E-4	3.571306491053622E-4
12	0.0	0.0	0.0	2.550933207895444E-4	4.5916797742118E-4
13	0.0	0.0	0.0	2.550933207895444E-4	5.101866415790888E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTTAA	35	0.0021812145	16.27368	1
AACCGCG	175	0.0	13.029588	7
CGAACGA	145	7.2759576E-12	12.450202	16
AATCGCT	170	0.0	12.295705	15
ACGAACG	140	5.2750693E-11	12.215863	15
CTTATAC	180	0.0	12.134875	3
ACTGCGC	55	0.00306773	12.091543	8
CCGTCGT	215	0.0	11.931163	9
CGTTATT	185	0.0	11.805398	2
ATCGCCA	330	0.0	11.804855	16
GTATTAG	405	0.0	11.7197275	1
TAACGAA	155	2.5465852E-11	11.646665	13
GCATCGC	345	0.0	11.566711	14
AAATCGC	165	7.2759576E-12	11.516638	14
ATTTAGA	190	0.0	11.491501	1
AGAACCG	240	0.0	11.478303	5
CGGTCCA	425	0.0	11.401181	10
TCCAATA	260	0.0	11.321732	2
TCGCCAG	345	0.0	11.292467	17
CGCATCG	345	0.0	11.291313	13
>>END_MODULE