FastQCFastQC Report
Thu 2 Feb 2017
SRR4062458_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062458_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1960067
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC20140.1027515896140285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG3250.020.30762135
TAACGGC3450.019.76804736
CGCAATA5250.018.01898636
ATACCGT5200.017.7691696
GTATCAA36850.017.6742841
ACCGATA750.001291289117.59993736
ACCGTCG5100.017.2548438
TCCTACA7650.017.254842
CCGTCGT5000.017.159949
CGTCGTA4900.017.06116510
TACCGTC5300.017.018817
TAGGACG4950.016.888834
ATTGTCG3550.016.73233643
GTCGTAA3700.016.64859230
ATACGAA5750.016.45211840
GTCCTAC8100.016.2987351
CTAGCGG5750.016.0695129
TAGATTG1259.446489E-615.8399455
TCTAGCG5900.015.66096228
CAATACG6100.015.50814338
Produced by FastQC (version 0.11.5)