##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062458_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1960067
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.268323480778974	32.0	32.0	32.0	32.0	32.0
2	31.3706541664137	32.0	32.0	32.0	32.0	32.0
3	31.475409258969208	32.0	32.0	32.0	32.0	32.0
4	31.588499270688196	32.0	32.0	32.0	32.0	32.0
5	31.503935834846462	32.0	32.0	32.0	32.0	32.0
6	35.10441326750565	36.0	36.0	36.0	36.0	36.0
7	35.119790803069485	36.0	36.0	36.0	36.0	36.0
8	35.05405835616844	36.0	36.0	36.0	36.0	36.0
9	35.208643378006975	36.0	36.0	36.0	36.0	36.0
10	35.06177799024217	36.0	36.0	36.0	36.0	36.0
11	35.20770361421319	36.0	36.0	36.0	36.0	36.0
12	35.11314460168963	36.0	36.0	36.0	36.0	36.0
13	35.1670682685847	36.0	36.0	36.0	36.0	36.0
14	35.127999195945854	36.0	36.0	36.0	36.0	36.0
15	35.081562518015964	36.0	36.0	36.0	36.0	36.0
16	35.096082429835306	36.0	36.0	36.0	36.0	36.0
17	35.07507243374844	36.0	36.0	36.0	36.0	36.0
18	35.08501342045961	36.0	36.0	36.0	36.0	36.0
19	35.0685221474572	36.0	36.0	36.0	36.0	36.0
20	35.05919695602242	36.0	36.0	36.0	36.0	36.0
21	35.0586653415419	36.0	36.0	36.0	36.0	36.0
22	35.039365491077604	36.0	36.0	36.0	36.0	36.0
23	35.00338763930009	36.0	36.0	36.0	36.0	36.0
24	34.98208734701416	36.0	36.0	36.0	36.0	36.0
25	34.956385164384685	36.0	36.0	36.0	32.0	36.0
26	34.91511208545422	36.0	36.0	36.0	32.0	36.0
27	34.89818766399312	36.0	36.0	36.0	32.0	36.0
28	34.88323664446165	36.0	36.0	36.0	32.0	36.0
29	34.868610613820856	36.0	36.0	36.0	32.0	36.0
30	34.84932096708939	36.0	36.0	36.0	32.0	36.0
31	34.84186611988264	36.0	36.0	36.0	32.0	36.0
32	34.810754428292505	36.0	36.0	36.0	32.0	36.0
33	34.79172038506847	36.0	36.0	36.0	32.0	36.0
34	34.78142532882804	36.0	36.0	36.0	32.0	36.0
35	34.74920500166576	36.0	36.0	36.0	32.0	36.0
36	34.72417422465661	36.0	36.0	36.0	32.0	36.0
37	34.72162431182199	36.0	36.0	36.0	32.0	36.0
38	34.67778958576416	36.0	36.0	36.0	32.0	36.0
39	34.67449326987292	36.0	36.0	36.0	32.0	36.0
40	34.66398801673616	36.0	36.0	36.0	32.0	36.0
41	34.627016831567495	36.0	36.0	36.0	32.0	36.0
42	34.609624568956065	36.0	36.0	36.0	32.0	36.0
43	34.603623243491164	36.0	36.0	36.0	32.0	36.0
44	34.5613991766608	36.0	36.0	36.0	32.0	36.0
45	34.52724932362006	36.0	36.0	36.0	32.0	36.0
46	34.528273778396354	36.0	36.0	36.0	32.0	36.0
47	34.50100175147074	36.0	36.0	36.0	32.0	36.0
48	34.46450503987874	36.0	36.0	36.0	32.0	36.0
49	34.443276683909275	36.0	36.0	36.0	32.0	36.0
50	33.931106436667726	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	12.0
21	60.0
22	138.0
23	402.0
24	1090.0
25	2680.0
26	5768.0
27	11213.0
28	18458.0
29	28911.0
30	42674.0
31	62358.0
32	93454.0
33	157940.0
34	350648.0
35	1184257.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.55380659659378	18.25832534344609	11.703879385784752	26.483988674175375
2	16.36742209530554	20.46208375446622	36.648739713600726	26.521754436627514
3	18.383195131147097	24.11156648474963	28.804862730612623	28.70037565349065
4	12.365610324147413	15.71126467744692	36.17395341481022	35.74917158359544
5	14.38889588978336	36.72063250899076	33.55732227520794	15.333149326017937
6	34.46132889128111	35.547369844213556	16.5886161357717	13.402685128733632
7	30.21280916050786	30.57432759917268	20.864501501480053	18.348361738839404
8	28.092458063933528	33.05892094504932	19.46341630158561	19.385204689431536
9	27.500386466380995	14.149057149577029	18.653341952086333	39.69721443195564
10	15.877008285941246	26.60225390254517	31.794627428552186	25.726110382961398
11	37.434281583231595	21.10932942598391	22.323930763591246	19.132458227193254
12	24.568037725241023	23.808216759937288	28.73901759480671	22.88472792001498
13	29.479910635707864	19.424693135489758	25.350613014759187	25.744783214043192
14	23.403128566523492	19.705244769694097	25.09705025389438	31.79457640988803
15	25.093989134044907	27.403553041809285	22.214852859621637	25.287604964524174
16	25.820137781004426	25.744885251371507	23.843317600877928	24.59165936674614
17	23.989333017697863	25.896104571935552	25.36112285957572	24.753439550790866
18	24.919556321289015	24.7310933758897	26.380067620137478	23.969282682683808
19	25.527341667402187	25.14230380900245	25.414794494269838	23.915560029325526
20	25.597849461268417	24.47125531933347	25.15633394164587	24.77456127775224
21	26.556388123467205	24.34687181611649	24.48957101976616	24.607169040650138
22	25.586676373817834	24.726195584130544	24.903995628720853	24.78313241333077
23	24.339474109813594	24.362789639333755	25.60682874616021	25.69090750469244
24	24.770989971261187	25.22240311173036	25.148272992708925	24.85833392429953
25	24.83369190951126	24.687064268721425	25.408774291899206	25.070469529868113
26	24.434572899803936	25.29602304411023	25.824933535435267	24.44447052065057
27	25.231127303301363	25.01756317513636	24.955524479520346	24.795785042041928
28	24.408298287762612	24.800478759144458	25.794475392933	24.996747560159932
29	24.490948523698425	25.06562275677311	25.55371831677182	24.88971040275664
30	24.547273128928758	24.972258601364135	25.883349905896075	24.59711836381103
31	24.9229745717876	24.904454796698275	24.920423638579702	25.252146992934428
32	24.578037383415975	25.0239405081561	25.008277778259618	25.389744330168305
33	24.30840374334143	24.63823940712231	25.534382243055976	25.51897460648029
34	24.893791895889272	24.764918750226393	25.874013490355175	24.467275863529157
35	25.647898770807327	24.658085667479735	25.42407989114658	24.269935670566365
36	24.469724759408734	25.111641591843544	25.323930253404605	25.09470339534312
37	25.460150086706218	24.952718452991657	24.816447601025885	24.77068385927624
38	24.49834623000132	24.87047636636911	25.46111944132522	25.170057962304348
39	24.968126089567345	24.742419519332756	24.95317762096908	25.336276770130816
40	25.324542477374496	24.779152957526453	25.574125782434987	24.32217878266406
41	24.375391249380762	25.008226759595466	25.821260191615895	24.795121799407877
42	25.42015145400642	25.268115834815852	25.26694240554022	24.044790305637513
43	24.479673398919527	24.471969580631683	25.65907185825791	25.389285162190884
44	24.31059754590022	24.870068217055845	25.4455077300929	25.373826506951037
45	24.370391420293284	25.155364587026874	25.54019837076998	24.934045621909863
46	24.349931609341127	24.655278937462725	25.588259152894576	25.406530300301572
47	24.617780922794985	24.51936591963438	25.87003403455086	24.992819123019775
48	25.020675313649992	25.37693864546467	24.9497593704705	24.652626670414836
49	24.1753470672176	25.193373491824516	25.451119783150272	25.180159657807614
50	24.056294051472783	25.637721198021495	25.16605316660417	25.139931583901554
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	45.0
1	58.0
2	71.0
3	167.0
4	263.0
5	290.5
6	318.0
7	300.5
8	283.0
9	405.0
10	527.0
11	736.0
12	945.0
13	1747.5
14	2550.0
15	3251.0
16	3952.0
17	4650.0
18	5348.0
19	5884.5
20	6421.0
21	7510.0
22	8599.0
23	9320.5
24	10042.0
25	12371.5
26	14701.0
27	18246.0
28	21791.0
29	25004.0
30	28217.0
31	30678.0
32	33139.0
33	38537.5
34	43936.0
35	50883.0
36	57830.0
37	68136.5
38	78443.0
39	83333.5
40	88224.0
41	96572.5
42	104921.0
43	105737.0
44	106553.0
45	116754.0
46	126955.0
47	133988.5
48	141022.0
49	143804.5
50	146587.0
51	141070.5
52	135554.0
53	133330.5
54	131107.0
55	132243.0
56	133379.0
57	130469.5
58	127560.0
59	116323.0
60	105086.0
61	93018.0
62	80950.0
63	70939.5
64	60929.0
65	52177.0
66	43425.0
67	38788.5
68	34152.0
69	31290.5
70	28429.0
71	22188.5
72	15948.0
73	14160.5
74	12373.0
75	9242.0
76	6111.0
77	5445.5
78	4780.0
79	4117.5
80	3455.0
81	2721.0
82	1987.0
83	1745.5
84	1504.0
85	1231.5
86	959.0
87	708.5
88	458.0
89	307.5
90	157.0
91	96.5
92	36.0
93	27.5
94	19.0
95	16.0
96	13.0
97	10.0
98	7.0
99	6.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015203561919056849
2	0.0038774184760010755
3	8.162986265265422E-4
4	1.0203732831581777E-4
5	0.0
6	6.122239698949066E-4
7	5.1018664157908884E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	2.0407465663163554E-4
47	0.0
48	0.0
49	0.0
50	1.0203732831581777E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1960067.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.101761266193805
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.01671764337102	44.89078790532839
2	12.492121413321485	14.016600264769389
3	3.4330662454282748	5.778031887361362
4	1.3407536043544193	3.0087455451312204
5	0.7014195891899426	1.9675437170082948
6	0.4192728102515692	1.4113165863683825
7	0.27571680968525647	1.0827739043827194
8	0.20766787382985796	0.9320426784208597
9	0.1470053105192511	0.7422531152052342
>10	0.7981516659497864	8.21054856885787
>50	0.07969159311848831	3.148281266752748
>100	0.07609892429489386	9.066792986748135
>500	0.009670746434778171	3.6634132831401516
>1k	0.002645770251024217	2.0808682905253706
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2014	0.1027515896140285	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5305599247372666E-4
2	0.0	0.0	0.0	0.0	1.5305599247372666E-4
3	0.0	0.0	0.0	0.0	1.5305599247372666E-4
4	0.0	0.0	0.0	0.0	1.5305599247372666E-4
5	0.0	0.0	0.0	0.0	2.0407465663163556E-4
6	0.0	0.0	0.0	0.0	2.0407465663163556E-4
7	0.0	0.0	0.0	5.101866415790889E-5	2.0407465663163556E-4
8	0.0	0.0	0.0	5.101866415790889E-5	2.0407465663163556E-4
9	0.0	0.0	0.0	5.101866415790889E-5	2.0407465663163556E-4
10	0.0	0.0	0.0	2.0407465663163556E-4	2.0407465663163556E-4
11	0.0	0.0	0.0	2.0407465663163556E-4	2.0407465663163556E-4
12	0.0	0.0	0.0	2.0407465663163556E-4	5.101866415790888E-4
13	0.0	0.0	0.0	2.0407465663163556E-4	5.101866415790888E-4
14	0.0	0.0	0.0	2.0407465663163556E-4	5.101866415790888E-4
15	0.0	0.0	0.0	2.0407465663163556E-4	7.142612982107244E-4
16	0.0	0.0	0.0	2.550933207895444E-4	7.142612982107244E-4
17	0.0	0.0	0.0	2.550933207895444E-4	7.652799623686334E-4
18	0.0	0.0	0.0	2.550933207895444E-4	8.162986265265423E-4
19	0.0	0.0	0.0	3.571306491053622E-4	8.162986265265423E-4
20	0.0	0.0	0.0	7.142612982107244E-4	9.1833595484236E-4
21	0.0	0.0	0.0	8.673172906844511E-4	9.1833595484236E-4
22	0.0	0.0	0.0	0.0011734292756319044	9.1833595484236E-4
23	0.0	0.0	0.0	0.0019897279021584466	9.693546190002689E-4
24	0.0	0.0	0.0	0.003265194506106169	9.693546190002689E-4
25	0.0	0.0	0.0	0.003979455804316893	9.693546190002689E-4
26	0.0	0.0	0.0	0.004846773095001344	9.693546190002689E-4
27	0.0	0.0	0.0	0.0069895569896335176	9.693546190002689E-4
28	0.0	0.0	0.0	0.01234651672621395	9.693546190002689E-4
29	0.0	0.0	0.0	0.02209108158037455	9.693546190002689E-4
30	0.0	0.0	0.0	0.04071289399801129	9.693546190002689E-4
31	0.0	0.0	0.0	0.08683376639676092	9.693546190002689E-4
32	0.0	0.0	0.0	0.13871974784535426	9.693546190002689E-4
33	0.0	0.0	0.0	0.1917791585695795	9.693546190002689E-4
34	0.0	0.0	0.0	0.25182812628343826	0.0010203732831581777
35	0.0	0.0	0.0	0.32253999480629997	0.0010203732831581777
36	0.0	0.0	0.0	0.42666908835259204	0.0010203732831581777
37	0.0	5.101866415790889E-5	0.0	0.5741640464331066	0.0010203732831581777
38	0.0	5.101866415790889E-5	0.0	0.7748204525661623	0.0010713919473160866
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAACGG	325	0.0	20.307621	35
TAACGGC	345	0.0	19.768047	36
CGCAATA	525	0.0	18.018986	36
ATACCGT	520	0.0	17.769169	6
GTATCAA	3685	0.0	17.674284	1
ACCGATA	75	0.0012912891	17.599937	36
ACCGTCG	510	0.0	17.254843	8
TCCTACA	765	0.0	17.25484	2
CCGTCGT	500	0.0	17.15994	9
CGTCGTA	490	0.0	17.061165	10
TACCGTC	530	0.0	17.01881	7
TAGGACG	495	0.0	16.88883	4
ATTGTCG	355	0.0	16.732336	43
GTCGTAA	370	0.0	16.648592	30
ATACGAA	575	0.0	16.452118	40
GTCCTAC	810	0.0	16.298735	1
CTAGCGG	575	0.0	16.06951	29
TAGATTG	125	9.446489E-6	15.839945	5
TCTAGCG	590	0.0	15.660962	28
CAATACG	610	0.0	15.508143	38
>>END_MODULE