FastQCFastQC Report
Thu 2 Feb 2017
SRR4062457_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062457_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1025503
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG107921.052361621565222No Hit
TCCATGTACTCTGCGTTGATACCAC85990.8385153432023115No Hit
GAGTACATGGAAGCAGTGGTATCAA42340.41287056205588873No Hit
CATGTACTCTGCGTTGATACCACTG35930.35036465032281716No Hit
GTATCAACGCAGAGTACTTTTTTTT31610.30823898126090316No Hit
GTACTTTTTTTTTTTTTTTTTTTTT26540.2587998279868513No Hit
GCTTCCATGTACTCTGCGTTGATAC26330.25675205240745275No Hit
TATCAACGCAGAGTACTTTTTTTTT24910.24290518896580507No Hit
CCCATGTACTCTGCGTTGATACCAC24790.241735031491863No Hit
GTACATGGGAAGCAGTGGTATCAAC24100.23500662601669617No Hit
GCGTTGATACCACTGCTTCCATGTA22950.22379261689141816No Hit
CATGGAAGCAGTGGTATCAACGCAG22150.21599156706513778No Hit
GAGTACTTTTTTTTTTTTTTTTTTT18320.17864404102182052No Hit
GTATCAACGCAGAGTACATGGAAGC16350.1594339558246051No Hit
ACTCTGCGTTGATACCACTGCTTCC14270.13915122627627613No Hit
ACGCAGAGTACTTTTTTTTTTTTTT14040.13690842445122053No Hit
CAGTGGTATCAACGCAGAGTACATG13710.13369049139787986No Hit
ACGCAGAGTACATGGAAGCAGTGGT13690.13349546515222288No Hit
GTACATGGGGTGGTATCAACGCAAA12230.11925854921926118No Hit
TATCAACGCAGAGTACATGGAAGCA11750.11457791932349295No Hit
GGTATCAACGCAGAGTACTTTTTTT11200.1092146975679252No Hit
GTCTTGCGCCGGTCCAAGAATTTCA11100.10823956633964014No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG10400.10141364774164482No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT802.0066855E-613.0571671
CGTCGCC604.0905332E-412.66643814
TATGACT604.093903E-412.6652024
CCGTCGT1301.9645086E-1012.4240679
CGACCCG1155.318725E-912.3904775
AGGCCCG2400.012.27181110
CGCCAGT1551.8189894E-1212.26023818
AACCGCG701.0893899E-412.215267
CTAACCT550.00306987512.0895124
CTTAGGT550.003072120212.0883323
TACGCTA1850.011.8117519
TTAAGCT658.013966E-411.69323911
AAGCTCG658.013966E-411.6932397
TGACCGT658.023818E-411.6915275
TTCTAAT658.040261E-411.6886752
GTGCTAG658.046846E-411.6875341
GTTATTC907.471219E-611.6086363
CGTTATT907.4793406E-611.6075032
ACGCTAT1900.011.50091610
AAGACGG2150.011.4876055
Produced by FastQC (version 0.11.5)