##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062457_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1025503
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.070528316348174	32.0	32.0	32.0	32.0	32.0
2	30.734829639698763	32.0	32.0	32.0	32.0	32.0
3	30.725355264684744	32.0	32.0	32.0	32.0	32.0
4	30.777929464857735	32.0	32.0	32.0	32.0	32.0
5	30.577103138654884	32.0	32.0	32.0	32.0	32.0
6	34.21984528567932	36.0	36.0	36.0	32.0	36.0
7	34.049174892711186	36.0	36.0	36.0	32.0	36.0
8	34.04574340591885	36.0	36.0	36.0	32.0	36.0
9	34.20913249400538	36.0	36.0	36.0	32.0	36.0
10	33.880663440282476	36.0	36.0	36.0	32.0	36.0
11	34.24606948980159	36.0	36.0	36.0	32.0	36.0
12	33.988615342909775	36.0	36.0	36.0	32.0	36.0
13	34.10786804134166	36.0	36.0	36.0	32.0	36.0
14	34.01538171999497	36.0	36.0	36.0	32.0	36.0
15	33.95608399000295	36.0	36.0	36.0	32.0	36.0
16	33.93959744632634	36.0	36.0	36.0	32.0	36.0
17	33.8538804859664	36.0	36.0	36.0	32.0	36.0
18	33.87881361634242	36.0	36.0	36.0	32.0	36.0
19	33.85954892379642	36.0	36.0	36.0	32.0	36.0
20	33.85468204383605	36.0	36.0	36.0	32.0	36.0
21	33.79044234877909	36.0	36.0	36.0	32.0	36.0
22	33.740970041043276	36.0	36.0	36.0	32.0	36.0
23	33.66712530338771	36.0	36.0	36.0	27.0	36.0
24	33.646068319644115	36.0	36.0	36.0	27.0	36.0
25	33.11007963896741	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	7.0
4	47.0
5	111.0
6	170.0
7	38.0
8	157.0
9	169.0
10	104.0
11	38.0
12	64.0
13	58.0
14	215.0
15	270.0
16	410.0
17	547.0
18	763.0
19	1053.0
20	1768.0
21	2407.0
22	3680.0
23	5264.0
24	7183.0
25	10042.0
26	13480.0
27	17349.0
28	23219.0
29	30811.0
30	39850.0
31	53811.0
32	77942.0
33	109295.0
34	231761.0
35	393420.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.743480720095185	16.79594702658423	11.215794503715552	25.244777749605042
2	15.801924970988912	20.4956751538319	37.21347284659717	26.488927028582015
3	19.112895911937112	25.14176716735791	28.146012137122373	27.599324783582606
4	12.308685598254629	15.644453893875307	35.52213361213489	36.52472689573518
5	13.849446505983668	37.37742946219189	33.97530445097374	14.797819580850707
6	34.024766321860724	34.700264677504165	17.615053740362395	13.659915260272717
7	29.43006354311408	30.84143885341369	21.614221479758317	18.114276123713914
8	28.787745700845193	32.32271476254611	20.240967383029805	18.648572153578886
9	27.307731348994103	14.689485006188047	18.401141567664432	39.60164207715342
10	15.29296913134311	27.544955776402368	32.47124978034637	24.690825311908153
11	37.04535691971259	21.462335988753516	21.502753045923146	19.989554045610745
12	23.488209518304732	24.34222859240629	29.491142493380302	22.678419395908676
13	30.324183001431226	19.83778158309398	25.121497845353595	24.716537570121194
14	23.78510256920932	19.62180410901664	25.41101881899942	31.182074502774622
15	25.565739622615723	26.47801032247232	24.09429433005944	23.861955724852518
16	25.799171226224004	25.6176024131081	24.757395756561127	23.825830604106773
17	23.814009975466483	25.72817097898787	26.612666245571422	23.845152799974226
18	25.16690782626355	24.164289243110353	27.6789706878809	22.989832242745198
19	24.38569307061198	27.23337072826383	25.61679667019136	22.764139530932834
20	24.372232879855122	24.015600822020787	27.668907210254755	23.943259087869336
21	25.71386449974567	25.36297326138468	24.205344598823135	24.717817640046512
22	24.302164793012665	25.563951626133765	25.267181237939734	24.86670234291384
23	23.121824139856972	25.15173825249089	25.490268832533307	26.236168775118834
24	23.248504660304516	26.910060907143457	25.30477981452515	24.536654618026873
25	24.929629307794524	24.965461488216874	24.849958846691965	25.254950357296636
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	169.0
1	169.0
2	501.0
3	833.0
4	833.0
5	833.0
6	2290.0
7	3747.0
8	3747.0
9	3747.0
10	3703.0
11	3659.0
12	3659.0
13	3659.0
14	3523.0
15	3387.0
16	3387.0
17	3387.0
18	5455.5
19	7524.0
20	7524.0
21	7524.0
22	11888.0
23	16252.0
24	16252.0
25	16252.0
26	23651.5
27	31051.0
28	31051.0
29	31051.0
30	37974.5
31	44898.0
32	44898.0
33	44898.0
34	54075.5
35	63253.0
36	63253.0
37	63253.0
38	71668.5
39	80084.0
40	80084.0
41	80084.0
42	94896.0
43	109708.0
44	109708.0
45	109708.0
46	139721.0
47	169734.0
48	169734.0
49	169734.0
50	154105.0
51	138476.0
52	138476.0
53	138476.0
54	122860.0
55	107244.0
56	107244.0
57	107244.0
58	97163.5
59	87083.0
60	87083.0
61	87083.0
62	77084.0
63	67085.0
64	67085.0
65	67085.0
66	55401.0
67	43717.0
68	43717.0
69	43717.0
70	32772.0
71	21827.0
72	21827.0
73	21827.0
74	17053.5
75	12280.0
76	12280.0
77	12280.0
78	10064.5
79	7849.0
80	7849.0
81	7849.0
82	5458.0
83	3067.0
84	3067.0
85	3067.0
86	2257.5
87	1448.0
88	1448.0
89	1448.0
90	990.5
91	533.0
92	533.0
93	533.0
94	317.0
95	101.0
96	101.0
97	101.0
98	297.5
99	494.0
100	494.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008093589194765886
2	0.003217933053340653
3	0.005265708632739251
4	0.015797125898217752
5	0.03695747355200326
6	0.04700132520333924
7	0.0669915153831827
8	0.0861040874575696
9	0.0923449273185939
10	0.1134077618495509
11	0.11535802430612101
12	0.12549938908028546
13	0.11750331300834808
14	0.11886849672794715
15	0.11077490753318127
16	0.10706940886569809
17	0.11604061616592053
18	0.12530436283462845
19	0.12676705967705604
20	0.11682072114854856
21	0.12228145602694483
22	0.14422190866335838
23	0.13369049139787986
24	0.12881483525645465
25	0.12520684971179996
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1025503.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.092270782797634
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.0689359291144	41.97949356856219
2	13.060554133919785	13.868289533029127
3	3.8024251699469187	6.056381602624414
4	1.4458391824462602	3.070515415312624
5	0.710868333044883	1.8870807014467454
6	0.4242910331141415	1.3515944652485379
7	0.2660049706873262	0.9885965553311169
8	0.17908920891501887	0.7606602219194559
9	0.13014787631867125	0.6218861662185257
>10	0.7254274540790058	7.250674943677611
>50	0.08365287495430143	3.130437987725666
>100	0.08897619948647557	9.483912100399944
>500	0.009375591101544765	3.374304624414305
>1k	0.004044372632038918	4.283567087709563
>5k	1.8383511963813266E-4	0.8392816575650414
>10k+	1.8383511963813266E-4	1.0533233688152026
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	10792	1.052361621565222	No Hit
TCCATGTACTCTGCGTTGATACCAC	8599	0.8385153432023115	No Hit
GAGTACATGGAAGCAGTGGTATCAA	4234	0.41287056205588873	No Hit
CATGTACTCTGCGTTGATACCACTG	3593	0.35036465032281716	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3161	0.30823898126090316	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2654	0.2587998279868513	No Hit
GCTTCCATGTACTCTGCGTTGATAC	2633	0.25675205240745275	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2491	0.24290518896580507	No Hit
CCCATGTACTCTGCGTTGATACCAC	2479	0.241735031491863	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2410	0.23500662601669617	No Hit
GCGTTGATACCACTGCTTCCATGTA	2295	0.22379261689141816	No Hit
CATGGAAGCAGTGGTATCAACGCAG	2215	0.21599156706513778	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1832	0.17864404102182052	No Hit
GTATCAACGCAGAGTACATGGAAGC	1635	0.1594339558246051	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1427	0.13915122627627613	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1404	0.13690842445122053	No Hit
CAGTGGTATCAACGCAGAGTACATG	1371	0.13369049139787986	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	1369	0.13349546515222288	No Hit
GTACATGGGGTGGTATCAACGCAAA	1223	0.11925854921926118	No Hit
TATCAACGCAGAGTACATGGAAGCA	1175	0.11457791932349295	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1120	0.1092146975679252	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1110	0.10823956633964014	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1040	0.10141364774164482	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	9.751312282850465E-5	9.751312282850465E-5
2	0.0	0.0	0.0	9.751312282850465E-5	9.751312282850465E-5
3	0.0	0.0	0.0	9.751312282850465E-5	9.751312282850465E-5
4	0.0	0.0	0.0	2.9253936848551394E-4	9.751312282850465E-5
5	0.0	0.0	0.0	2.9253936848551394E-4	9.751312282850465E-5
6	0.0	0.0	0.0	2.9253936848551394E-4	9.751312282850465E-5
7	0.0	0.0	0.0	3.900524913140186E-4	9.751312282850465E-5
8	0.0	0.0	0.0	5.850787369710279E-4	9.751312282850465E-5
9	0.0	0.0	0.0	6.825918597995325E-4	9.751312282850465E-5
10	0.0	0.0	0.0	6.825918597995325E-4	9.751312282850465E-5
11	0.0	0.0	0.0	6.825918597995325E-4	9.751312282850465E-5
12	0.0	0.0	0.0	6.825918597995325E-4	9.751312282850465E-5
13	0.0	0.0	0.0	7.801049826280372E-4	9.751312282850465E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAT	80	2.0066855E-6	13.057167	1
CGTCGCC	60	4.0905332E-4	12.666438	14
TATGACT	60	4.093903E-4	12.665202	4
CCGTCGT	130	1.9645086E-10	12.424067	9
CGACCCG	115	5.318725E-9	12.390477	5
AGGCCCG	240	0.0	12.271811	10
CGCCAGT	155	1.8189894E-12	12.260238	18
AACCGCG	70	1.0893899E-4	12.21526	7
CTAACCT	55	0.003069875	12.089512	4
CTTAGGT	55	0.0030721202	12.088332	3
TACGCTA	185	0.0	11.811751	9
TTAAGCT	65	8.013966E-4	11.693239	11
AAGCTCG	65	8.013966E-4	11.693239	7
TGACCGT	65	8.023818E-4	11.691527	5
TTCTAAT	65	8.040261E-4	11.688675	2
GTGCTAG	65	8.046846E-4	11.687534	1
GTTATTC	90	7.471219E-6	11.608636	3
CGTTATT	90	7.4793406E-6	11.607503	2
ACGCTAT	190	0.0	11.500916	10
AAGACGG	215	0.0	11.487605	5
>>END_MODULE