FastQCFastQC Report
Thu 2 Feb 2017
SRR4062457_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062457_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1025503
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA39560.3857619139095644No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC37200.3627488169220373No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA14710.14344180368073034No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC14130.13778604255667706No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA11060.10784951384832614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATA756.184564E-1132.26634644
TAGGACG959.622454E-1025.4734294
GTATTAC1105.2368705E-922.0051461
TTAACGG956.864866E-720.84189635
GGACCGT808.9875684E-519.2498076
TGGTATA3250.018.95365744
GTATTAT1051.7881412E-618.8615531
TAACGGC1051.7923176E-618.85695536
TGCGCCT600.00741178118.3331510
TACACCG600.00741178118.333155
GTCTTAG1601.0968506E-917.879181
TCTATAC1509.586074E-917.6006833
TGCGTCC750.001290775417.59982526
CTCGTAT902.2151273E-417.1109443
CGAGCCG3500.016.97125815
GTCGTAT953.3459498E-416.21036343
GATATAA1105.60868E-516.0037421
GGCGTTA2750.015.9998442
ATTACAC1958.913048E-1015.7954843
GTATTAG2800.015.717961
Produced by FastQC (version 0.11.5)